Protection against Virulent Infectious Bronchitis Virus Challenge Conferred by a Recombinant Baculovirus Co-Expressing S1 and N ProteinsArticle Published on 2018-06-272022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] age antibody baculovirus booster bronchitis candidate vaccine CD4+ CD8+ T lymphocyte CD8+ T lymphocytes challenge co-expressing control vaccine demonstrated develop dose evaluated FIVE IBV IBV infection immune protection imperative Infectious Bronchitis Virus kidney morbidity Mortality N protein percentage protection recombinant Safe specific pathogen-free SPF subunit Subunit vaccine Trachea Vaccine vaccine strain Vaccines virulent virus was used [DOI] 10.3390/v10070347 PMC 바로가기 [Article Type] Article
Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral ReplicationArticle Published on 2018-03-132022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] cells cellular cytoplasm demonstrated epidemic diarrhea expression heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) in viral in vivo infected cells Infection inhibited multifunctional protein N protein nuclear nucleocapsid nucleocapsid protein nucleus PEDV porcine epidemic diarrhea virus (PEDV) positive pre-mRNA predicted Protein proteomic study Regulation Replication ribonucleoprotein thought translation virus [DOI] 10.3390/v10030127 PMC 바로가기 [Article Type] Article
The nucleocapsid proteins of mouse hepatitis virus and severe acute respiratory syndrome coronavirus share the same IFN-β antagonizing mechanism: attenuation of PACT-mediated RIG-I/MDA5 activationResearch Paper Published on 2017-07-252022-10-28 Journal: Oncotarget [Category] Coronavirus, [키워드] activate Activation acute respiratory syndrome antiviral response association cellular circumvent coronavirus CoV CoV-infected cells CoVs epidemic diarrhea Human IFN IFN-β IFNs inhibited Innate immunity innate immunity evasion Interaction interferon MDA5 mechanism Melanoma MHV molecular mouse hepatitis virus N protein N proteins Nidovirale nucleocapsid protein PACT PEDV Protein reproductive RIG-I RIG-I/MDA5 SARS-CoV N protein Sendai virus syndrome the N protein turn type I interferon upstream Viral protein virus [DOI] 10.18632/oncotarget.17912 PMC 바로가기 [Article Type] Research Paper
Nucleocapsid Interacts with NPM1 and Protects it from Proteolytic Cleavage, Enhancing Cell Survival, and is Involved in PEDV GrowthArticle Published on 2017-01-032022-10-29 Journal: Scientific Reports [Category] Coronavirus, MERS, [키워드] activities addition binding caspase-3 Cell components cytoplasm Degradation ENhance Enhancing epidemic diarrhea growth host cell independent indicated infected cells interact Interaction knockdown mechanism N protein NPM1 nuclear translocation nucleocapsid Nucleolus nucleophosmin nucleus overexpression PEDV Prevent promoted PROTECT Protein proteolytic proteolytic cleavage Regulation replicate Replication required suppressed survival the N protein virus [DOI] 10.1038/srep39700 PMC 바로가기 [Article Type] Article
The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea VirusArticle Published on 2016-12-192022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] addition Analysis Antisera cause characterization conserved contagious contribute coronavirus cross-reactivity death diagnostic diarrhea epidemic diarrhea epitope Freeze-thaw cycle hyperimmune identification IFA indicated indirect immunofluorescence intrinsically monoclonal antibody N protein Neonatal novel nucleocapsid protein overlapping PEDV protease residue screened significantly strain TGEV the N protein Treatment virus [DOI] 10.1038/srep39010 PMC 바로가기 [Article Type] Article
Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23Article Published on 2016-05-072022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] Analysis Antibody assays antibody tests Antibody titer Camel cells characterization Cluster codon coronavirus correlation CoV Cytopathic effect Dromedary Dromedary camel Fecal sample FIVE Genetic heat-inactivated immunofluorescence Isolation MERS MERS-CoV mice middle Middle East motif N gene N protein neutralization Neutralization assay Northern blot analysis Pearson predicted RdRP reported respiratory syndrome coronavirus RNA separated sequence sera Seven strain subgenomic mRNA transcription regulatory sequence [DOI] 10.3390/ijms17050691 PMC 바로가기 [Article Type] Article
Comparative Genomic Analysis of Classical and Variant Virulent Parental/Attenuated Strains of Porcine Epidemic Diarrhea VirusArticle Published on 2015-10-232022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] aa deletion Amino acid analyzed Cell changes in characteristic characterized classical Comparative epidemic diarrhea etiological agent Genomic analysis marker molecular basis mutations N protein non-structural protein nsp10 nucleotide Open reading frame ORF ORF3 outbreak parental passage passages PEDV responsible S protein sequence sequenced strain Strains synonymous variant variant PEDV Variation Vero Cell virus virus attenuation virus cell adaptation [DOI] 10.3390/v7102891 PMC 바로가기 [Article Type] Article
Molecular Characterizations of Subcellular Localization Signals in the Nucleocapsid Protein of Porcine Epidemic Diarrhea VirusArticle Published on 2014-03-132022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Amino acid antibiotic Cell cellular characterization chromosome CHROMOSOME region maintenance 1 confocal microscopy coronavirus Critical cytoplasm epidemic diarrhea eukaryotic fluorescent full-length functional fusion protein GFP Infection inhibited Localization Mutation N protein NES nuclear nuclear export signal nucleocapsid nucleocapsid protein Nucleolus pathway PEDV Porcine epidemic diarrhea virus Protein signal site-directed mutagenesis subcellular localization signals the N protein VERO E6 cells virus virus replication [DOI] 10.3390/v6031253 PMC 바로가기 [Article Type] Article
Transient Oligomerization of the SARS-CoV N Protein – Implication for Virus Ribonucleoprotein PackagingResearch Article Published on 2013-05-232022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] acute respiratory syndrome Affect base C-terminal changes in Concentration coronavirus CTD cysteine residue domain electrostatic repulsion intrinsically mechanism mutant Mutation N protein nucleocapsid oligomer oligomerization Protein Region regulate SARS-CoV SARS-CoV N Transient viral genome [DOI] 10.1371/journal.pone.0065045 PMC 바로가기 [Article Type] Research Article
Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid proteinArticle Published on 2012-11-012022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] acute infection After alignment amino acid sequence Analysis antigenic structure assay Avian infectious bronchitis Cell-mediated immunity conserved coronavirus cross cross-reactive antibody diagnostic diagnostics epitope epitope mapping Epitopes facilitate Heterologous IBV immunogens indicated induce infections infectious Infectious Bronchitis Virus Influenza virus isolate linear B-cell epitope mAb mAbs monoclonal antibody N protein newcastle disease virus nucleocapsid nucleocapsid protein peptide Phylogenetic analysis PROTECT Protein Replication Respiratory disease sequence serotypes Strains the N protein translation viral particle Viral RNA virus [DOI] 10.1016/j.virusres.2012.10.028 PMC 바로가기 [Article Type] Article