In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity증가 된 감도와 SARS-COV-2의 검출을위한 사내 역전사 효소 중합 효소 연쇄 반응Article Published on 2021-09-092022-09-01 Journal: Scientific Reports [Category] SARS, 변종, 진단, 치료기술, 치료제, [키워드] added advent amplification amplify amplifying applicability approach concentrations Concordance COVID-19 disease COVID-19 infection COVID-19 test demonstrated Dilution Epidemiology help management multiplex Multiplex PCR Multiplexing offer pandemic PCR condition polymerase chain positive sample primer Primers RdRP real time Real-time PCR relief resource RT-PCR SARS-CoV-2 SARS-CoV-2 genes sensitivity sets of primers shown significantly SYBR Green the SARS-CoV-2 transcriptase variable [DOI] 10.1038/s41598-021-97502-1 PMC 바로가기 [Article Type] Article
Rapid and highly sensitive one-tube colorimetric RT-LAMP assay for visual detection of SARS-CoV-2 RNASARS-CoV-2 RNA의 시각적 검출을 위한 신속하고 고감도의 1관 비색 RT-LAMP 분석Article Published on 2021-09-012022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplifying bypassing caused clinics Community Concordance contagious contagious disease coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 demonstrated diagnostic diagnostic test disease gold gold standard hospitals imperative isothermal amplification LAMP less limit of detection magnetic bead One-tube outbreak performed platform point-of-care test Rapid RNA RNA extraction RNA molecule RT-LAMP RT-qPCR test sample collection SARS-CoV-2 SARS-CoV-2 RNA severe acute respiratory syndrome Coronavirus specificity specimen Visual Visual readout. Volume [DOI] 10.1016/j.bios.2021.113330 PMC 바로가기 [Article Type] Article
An electrochemical dual-aptamer biosensor based on metal-organic frameworks MIL-53 decorated with Au@Pt nanoparticles and enzymes for detection of COVID-19 nucleocapsid proteinResearch article Published on 2021-08-012022-10-05 Journal: Electrochimica Acta [Category] 치료기술, [키워드] 2019-nCov-NP amplifying applied Au@Pt/MIL-53 (Al) Biomarker COVID-19 demonstrated Detection limit detection system determine DNAZyme Dual-aptamer early diagnosis electrochemical biosensor elements enzyme G-quadruplex HRP hydrogen peroxide linear nucleocapsid protein peroxidase Protein reliability SARS-CoV-2 nucleocapsid sensitivity [DOI] 10.1016/j.electacta.2021.138553 [Article Type] Research article
Association of COVID-19 transmission with high levels of ambient pollutants: Initiation and impact of the inflammatory response on cardiopulmonary disease높은 수준의 주변 오염 물질과 COVID-19 전염의 연관성: 심폐 질환에 대한 염증 반응의 개시 및 영향Review Published on 2021-07-202022-09-11 Journal: The Science of the total environment [Category] SARS, 변종, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition air pollutant Air pollution ambient amplifying angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme-2 association Cardiopulmonary cardiopulmonary disease cardiopulmonary diseases Cardiopulmonary effects caused chronic component contribute coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 transmission cytokine release death Effects elevated endothelial exposure to expression fusion Hospitalization host cells implicated incidence Inflammatory response Inflammatory responses Initiation internalization lockdown lung epithelial mechanical ventilation mechanism Mutation other variants overexpression overlap oxidative oxidative stress pandemic Particulate matter pathophysiology Premature prolonged exposure receptor redox reductions in Region regions responsible S-protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 transmission spike glycoprotein the SARS-CoV-2 thrombosis Vascular vascular thrombosis while [DOI] 10.1016/j.scitotenv.2021.146464 PMC 바로가기 [Article Type] Review
Validation of real-time RT-PCR for detection of SARS-CoV-2 in the early stages of the COVID-19 outbreak in the Republic of Korea한국에서 COVID-19 발병 초기 단계에서 SARS-COV-2 탐지를위한 실시간 RT-PCR의 검증Article Published on 2021-07-202022-09-01 Journal: Scientific Reports [Category] 진단, 치료기술, 치료법, 치료제, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplifying authorization Chain Reaction clinical microbiology coefficient Coefficient of variation coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 outbreak demonstrated diagnosis of COVID-19 early diagnosis early stage early stages effective Emergency EUA facilitate implementation limit of detection linearity outbreak Patient Plasmid plasmids polymerase chain polymerase chain reaction Precision public health measures reagent reagents Real-Time Reverse Transcription Polymerase Chain Reaction real-time RT-PCR reproducibility respiratory respiratory diseases reverse transcription robustness RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 gene SARS-CoV-2 genes sensitivity severe acute respiratory syndrome Coronavirus specificity Treatment validation Variation Viral Virology virus viruses was tested [DOI] 10.1038/s41598-021-94196-3 PMC 바로가기 [Article Type] Article
Comparative performance and cycle threshold values of 10 nucleic acid amplification tests for SARS-CoV-2 on clinical samplesResearch Article Published on 2021-06-232022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] Accuracy amplifying approved assays Clinical practice clinical sample clinical settings Clinical use Comparative cycle threshold Cycle threshold value Diagnosis diagnostic difference Diseases E-gene eight Emergency use authorization false-positive result Genetic gold standard include increasingly Japan marker NAAT nine nucleic acid amplification positive result positive sample ranged reagent reagents receiving Research Result SARS-CoV-2 sensitivity Significant specificity while [DOI] 10.1371/journal.pone.0252757 PMC 바로가기 [Article Type] Research Article
Using Fear and Anxiety Related to COVID-19 to Predict Cyberchondria: Cross-sectional Survey StudyOriginal Paper Published on 2021-06-092022-10-30 Journal: Journal of Medical Internet Research [Category] COVID-19, MERS, [키워드] 95% CI addition adverse effect Affect amplification amplifying Anxiety anxiety sensitivity association concerning conducted convenience COVID-19 COVID-19 anxiety COVID-19 fear COVID-19 pandemic Cyberchondria effect sizes ENhance Factor fear female index intolerance intolerance of uncertainty investigated lack males media Mediation analysis mental health moderate Multiple objective populations predicted Psychological public health recruit reduce repeated Research Result scale SEM sensitivity significantly higher statistically significant survey total effect Uncertainty variables was used [DOI] 10.2196/26285 PMC 바로가기 [Article Type] Original Paper
A rigorous evaluation of optimal peptide targets for MS-based clinical diagnostics of Coronavirus Disease 2019 (COVID-19)Research Published on 2021-05-102022-11-01 Journal: Clinical Proteomics [Category] COVID-19, [키워드] amplifying Analysis analyzed assays automated bioinformatics Care Cell culture clinical samples collected coronavirus coronavirus 2 COVID-19 COVID-19 infection status Cutoff dataset detect detection Diagnosis diagnosis of SARS-CoV-2 diagnostic diagnostics Evidence global pandemic highest human host in vitro Infection Limits of detection mass spectrometry membrane protein molecular nucleocapsid pandemic Patient peptide Peptide-detection Proteins proteomics quantitation respiratory Result SARS-CoV-2 seeking sequence specificity Spread sudden supplementary material target the SARS-CoV-2 virus upper respiratory tract viral peptide Viral proteome Viral RNA Visualization with COVID-19 Workflows [DOI] 10.1186/s12014-021-09321-1 PMC 바로가기 [Article Type] Research
Subjective accelerated aging moderates the association between COVID-19 health worries and peritraumatic distress among older adultsEtiology Published on 2021-04-142022-10-31 Journal: Global Mental Health [Category] COVID-19, [키워드] accelerated Adults amplifying association Characteristics correlated COVID-19 COVID-19 pandemic distress examined faster global pandemic Health health worries Interaction Intervention measure moderate Older outbreak participant peritraumatic distress predicted questionnaire reported Result risk Subjective subjective accelerated aging Symptom [DOI] 10.1017/gmh.2021.13 PMC 바로가기 [Article Type] Etiology
An Italian Survey on Dietary Habits and Changes during the COVID-19 LockdownArticle Published on 2021-04-052022-10-31 Journal: Nutrients [Category] COVID-19, MERS, SARS, [키워드] addition adherence alcohol amplifying change changes changes in conducted contribute coronavirus coronavirus disease COVID-19 decrease diet dietary habit dietary habits and changes Emergency impacted increase in Italian Italian population lockdown MEDAS score Mediterranean diet adherence medium Modification outbreak pandemic Participants performed physical activity positive Public questionnaire reduction reported Responder responders survey World Health Organization [DOI] 10.3390/nu13041197 PMC 바로가기 [Article Type] Article