A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays균질한 면역분석을 위한 비율계량적 생물발광 센서의 플러그 앤 플레이 플랫폼Article Published on 2021-07-282022-09-10 Journal: Nature Communications [Category] SARS, 바이오마커, 진단, 치료기술, [키워드] anti-SARS-CoV-2 antibodies antibodies antibody Assay systems Bioanalytical chemistry Biochemical assays bioluminescent Biomarkers blood plasma calibrator clinical laboratories complex ELISA Fluorescent proteins homogeneous immunoassay immunoassays Incubation luciferase media Nanoluc luciferase optimization performed platform point-of-care Protein provide quantitative measurement robust SARS-CoV-2 spike protein solution Spike protein therapeutic therapeutic antibodies translation [DOI] 10.1038/s41467-021-24874-3 PMC 바로가기 [Article Type] Article
Uncommon Presentation of COVID-19 With Long Incubation Period and Only Gastrointestinal Symptoms in a Fully Vaccinated Patient: Is There a Relation?Gastroenterology Published on 2021-06-292022-10-31 Journal: Cureus [Category] COVID-19, [키워드] affected caused chest X-ray Coronavirus disease 2019 COVID-19 COVID-19 case COVID-19 infection diarrhea dose Fever Gastrointestinal Gastrointestinal symptom Incubation Incubation period Infection laboratory investigation lockdown Long long incubation manifestation Pain Patient Period Pfizer Physicians post vaccine presentation reported respiratory infection respiratory infectious disease respiratory symptoms Spread the disease Treatment unusual Vaccine worldwide pandemic [DOI] 10.7759/cureus.16028 PMC 바로가기 [Article Type] Gastroenterology
Structure-based Design of a Specific, Homogeneous Luminescence Enzyme Reporter Assay for SARS-CoV-2SARS-CoV-2에 대한 특정 균질 발광 효소 리포터 분석의 구조 기반 설계Article Published on 2021-06-252022-09-11 Journal: Journal of Molecular Biology [Category] MERS, SARS, 진단, 치료기술, [키워드] antibody applied assays binding C-terminal catalytic activity complementary Complementation COVID-19 detectable develop diagnostic enzyme epitope Epitopes Express Gaussia Luciferase high affinity Incubation luciferase other diseases pathogenic PCA promise Protein protein-fragment complementation assay recombinant recombinant antibodies reporter reporters S-protein SARS CoV SARS CoV-2 SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 virus sensitivity Specific specificity spike spike (S) protein steric the SARS-CoV-2 virus threat Treatment Trimers Viral viral spike Virus-like particle virus-like particles [DOI] 10.1016/j.jmb.2021.166983 PMC 바로가기 [Article Type] Article
Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2조작된 ACE2 수용체 요법은 SARS-CoV-2의 돌연변이 탈출을 극복합니다Article Published on 2021-06-212022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 진단, 치료제, [키워드] ACE2 ACE2 receptor administration Antibody therapy binding caused cell sorting Concentration Disulfide Effect escape mutant escape mutants Evidence evidence of fixing global pandemic hACE2 hamster hamsters Hoshino hydrogen hydrophobic packing Incubation interface lung medication medications Mutagenesis mutated Mutation neutralized overcome passages pathology protease Protease activity random mutagenesis RBD Receptor binding domain receptors SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 mutation SARS-CoV-2 mutations stability target therapeutic interventions therapeutic potential therapy Vaccinations Viral viral adaptation virus receptor virus titer virus titers wild type [DOI] 10.1038/s41467-021-24013-y PMC 바로가기 [Article Type] Article
Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assaysRT-PCR 진단 분석에 적합한 구아니듐 기반 운송 배지를 사용하여 타액에서 SARS-Cov-2 바이러스의 불 활성화Research Article Published on 2021-06-112022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료기술, [키워드] 1:1 abrogated buffer Cell culture chain Chain Reaction clinical sample clinical samples condition conditions Copan COVID-19 CPE Cytopathic effect detect detectable diagnostic Dilution downstream evaluated expand guanidinium hazard inactivated inactivation Incubation infectious limit of detection log media medium minute passages positive processing reaction remained respiratory respiratory sample Result room temperature RT-PCR Saliva sample collection SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA SARS-CoV-2 USA-WA1/2020 SARS-CoV-2 virus stability temperature tested Transport upper VeroE6 cell VeroE6 cells Viral Viral RNA virus [DOI] 10.1371/journal.pone.0252687 PMC 바로가기 [Article Type] Research Article
Protective Action of L. salivarius SGL03 and Lactoferrin against COVID-19 Infections in Human NasopharynxArticle Published on 2021-06-042022-10-30 Journal: Materials [Category] COVID-19, [키워드] L. salivarius Action addition Anti-viral block carried collected Complete conducted coronavirus COVID-19 dietary supplement experiment experiments growth healthy healthy volunteers host cells Human human nasopharynx Incubation infected with SARS-CoV-2 ingredient inhibitory effect lactoferrin MBC media medium microorganism nasopharynx oral mucosa Research Result Salistat SGL03 secretion Swab viral particle virus particle virus particles [DOI] 10.3390/ma14113086 PMC 바로가기 [Article Type] Article
SARS-CoV-2 proteins regulate inflammatory, thrombotic and diabetic responses in human arterial fibroblastsSARS-CoV-2 단백질은 인간 동맥 섬유아세포에서 염증, 혈전 및 당뇨병 반응을 조절합니다Article Published on 2021-06-012022-09-11 Journal: Clinical immunology (Orlando, Fla.) [Category] SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus cardiovascular Cell cellular response complement coronavirus COVID-19 determine Diabetic disease envelope proteins exposure to expression fibroblast ICAM-1 Incubation Inflammation Inflammatory Inflammatory response Interaction marker mechanism mechanisms nucleocapsid progression Proteins RAGE regulate response responses responsible SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significantly spike structural proteins Support thrombosis thrombotic tissue Tissue Factor up-regulated Vascular vascular disease [DOI] 10.1016/j.clim.2021.108733 PMC 바로가기 [Article Type] Article
Impaired performance of SARS-CoV-2 antigen-detecting rapid diagnostic tests at elevated and low temperatures고온 및 저온에서 SARS-CoV-2 항원 검출 신속 진단 검사의 성능 저하Article Published on 2021-05-012022-09-10 Journal: Journal of Clinical Virology [Category] 진단, 치료기술, [키워드] Ag-RDT Ag-RDTs ambient ambient temperatures analytical sensitivity Antigen Antigen-detecting rapid diagnostic tests Cell culture cell culture supernatant clinically complement condition conditions consequence COVID-19 culture supernatant diagnostic tests diagnostics eight elevated elevated temperature Emergency use exhibited exposure to false-negative false-positive result False-positive results FIVE Genome Health Organization Highlights impair Impaired include Incubation limit limits Limits of detection low temperature Low temperatures mean temperature mean temperatures molecular molecular diagnostics North not affected occurred operation pandemic quarantine ranged rapid antigen test Rapid diagnostic test RDTs reduce reduced respiratory respiratory virus respiratory viruses SARS-CoV-2 SARS-CoV-2 cell sensitivity South specificity storage temperature Temperature stability test results tested the WHO Transmission Tropics virus concentration Virus concentrations viruses Winter World Health Organization [DOI] 10.1016/j.jcv.2021.104796 PMC 바로가기 [Article Type] Article
The Stability of Model Human Coronaviruses on Textiles in the Environment and during Health Care Laundering환경 및 의료 세탁 중 직물에 대한 모델 인간 코로나바이러스의 안정성Research Article Published on 2021-04-282022-09-10 Journal: mSphere [Category] MERS, SARS, 치료기술, [키워드] (artificial saliva) able to transfer acting acute respiratory syndrome acute respiratory syndrome coronavirus Agitation artificial saliva Care conditions coronavirus coronavirus disease Coronavirus disease 2019 cotton COVID-19 Culture decontamination demonstrated detectable detergent determine Dilution Efficacy Environment Evidence fiber handling HCoV HCoV-229E HCoV-OC43 Health Health care Human human coronavirus human coronavirus OC43 human coronaviruses Incubation infection control Infective dose Infectivity laundering laundry log Low temperatures Model OC43 persist persistence plastic polyester provide reduction required Research respiratory risk Saliva SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spread stability stainless steel synthetic TCID TCID50 temperature textile textiles tissue tissue culture transfer transferred Transmission United Kingdom United States virus [DOI] 10.1128/mSphere.00316-21 PMC 바로가기 [Article Type] Research Article
Fluticasone Propionate Suppresses Poly(I:C)-Induced ACE2 in Primary Human Nasal Epithelial Cells플루티카손 프로피오네이트는 1차 인간 비강 상피 세포에서 폴리(I:C) 유도 ACE2를 억제합니다Cellular and Infection Microbiology Published on 2021-04-262022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, [키워드] ACE2 ACE2 expression ACE2 protein Activation Affect affected agonist analyses angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 cell entry collected Corticosteroid COVID-19 dissemination enzyme epithelial cells evaluate examined expression expression of gene fluticasone fluticasone propionate Fold change followed by Gene Expression Health health problem HNECs host cells Human immunoblot immunofluorescence incubated Incubation individuals Infection initial innate immune interferon intranasal intranasal steroid spray investigated mRNA expression nasal Nasal cavity nasal epithelial cell nasal mucosa NFκB objective poly(I:C poly(I:C) promote propionate protease Protein quantitative PCR receptor regulated required reservoir Result SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 viral SARS-CoV2 Serine serine 2 significantly increased Spread steroid suppresse suppressed thought TLR TLR agonists TLR3 TMPRSS2 Toll-like receptors transmembrane transmembrane protease serine 2 Viral virus infection [DOI] 10.3389/fcimb.2021.655666 PMC 바로가기 [Article Type] Cellular and Infection Microbiology