An easy pipeline for one-step purification of SARS-CoV-2 nucleocapsid protein from insect cell suspension culture곤충 세포 현탁액 배양에서 SARS-CoV-2 뉴클레오캡시드 단백질의 1단계 정제를 위한 간편한 파이프라인Article Published on 2022-01-012022-09-11 Journal: Journal of virological methods [Category] SARS, 변종, 치료기술, 치료법, [키워드] affinity Affinity chromatography average baculovirus buffer cellular response Cellular responses cloned COVID-19 COVID-19 pandemic Culture expression of SARS-CoV-2 followed by Genome high-density imidazole Infection insect cell insect cells N gene N protein nucleocapsid nucleocapsid protein One-step purification Protein purification purified resulting SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 variant SARS-CoV-2 variants Seroconversion serological test Serological tests Sf9 cell supernatant total protein Transposition tris Tris-HCl vaccination was used [DOI] 10.1016/j.jviromet.2021.114341 PMC 바로가기 [Article Type] Article
Evaluation of a downstream process for the recovery and concentration of a Cell-Culture-Derived rVSV-Spike COVID-19 vaccine candidate세포 배양에서 파생된 rVSV-스파이크 COVID-19 백신 후보의 회수 및 농축을 위한 다운스트림 프로세스 평가Article Published on 2021-11-262022-09-12 Journal: Vaccine [Category] MERS, SARS, 진단, [키워드] animal models buffer Clarification clinical trial clinical trials Complete Concentration conditions Contamination COVID-19 pandemic COVID-19 vaccine Critical DNA dose downstream Downstream process endonuclease Endonuclease digestion evaluated examined experiment experiments filtration hcp Hollow fiber host cell induce less membrane neutralizing antibody nucleic acid parameter Particles PROTECT Protein purification reduce Regulatory required rVSV. SARS-CoV-2 SARS-COV-2 infection sensitivity Spike protein subsequent supernatant Treatment vaccine candidate was obtained [DOI] 10.1016/j.vaccine.2021.10.045 PMC 바로가기 [Article Type] Article
Pleural Mesothelial Cells Modulate the Inflammatory/Profibrotic Response During SARS-CoV-2 InfectionMolecular Biosciences Published on 2021-11-262022-10-31 Journal: Frontiers in Molecular Biosciences [Category] COVID-19, [키워드] ACE2 ACE2 expression ADAM17 analysed Analysis analyzed assays Cell confocal microscopy COVID-19 COVID-19 disease COVID-19 patients cytokeratin cytokine direct infection ELLA epithelial Evidence expressed expression fibrosis highlight immune response implicated in vivo Infection Inflammatory cytokine Inflammatory cytokines interferons Lung fibrosis marker MCs mesothelial cells mesothelial to mesenchymal transition morphology NRP1 Pathogenesis Patient plasma membrane pleura produced promoter pulmonary fibrosis receptor reported response RT-PCR SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 receptor SARS-CoV-2 replication shown supernatant sustained TMPRSS2 western blot WT1 [DOI] 10.3389/fmolb.2021.752616 PMC 바로가기 [Article Type] Molecular Biosciences
Discrimination of SARS-CoV-2 Infections From Other Viral Respiratory Infections by Scent Detection DogsMedicine Published on 2021-11-182022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] aetiology canine Cell Cell culture cell culture supernatant contribute coronavirus COVID-19 demonstrated detect detection detection dog diagnostic discrimination dog double-blind study evaluate include indicate individual infected individual infected with SARS-CoV-2 Infection infections investigated mandatory non-infected cell oropharyngeal swab Other other coronavirus performed positive randomised Research respiratory Saliva SARS-CoV-2 SARS-CoV-2-infected individual Scent scent detection dog screening method screening test sensitivities sensitivity specificity Spread supernatant swab sample Symptom Testing the mean variety viral infection viral infections viral respiratory virus volatile organic compound (VOC) [DOI] 10.3389/fmed.2021.749588 PMC 바로가기 [Article Type] Medicine
ProLung™-budesonide Inhibits SARS-CoV-2 Replication and Reduces Lung InflammationArticle Published on 2021-10-312022-10-28 Journal: Archives of pharmacology and therapeutics [Category] COVID-19, [키워드] administration AhR airway animal model Anti-inflammatory BAL benefit C57BL/6 Cell COVID-19 decrease determine Efficacy eosinophil EPO evaluate in vitro Inflammation inhibited low dose lung Lung inflammation Methacholine mice Microscopy offer patients with COVID-19 peroxidase protective effect SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2-infected cell SARS-CoV-2-infected cells significantly supernatant sustained therapy treated treatment for COVID-19 treatment group type II pneumocytes Vero viral replication virus was measured Weekly [DOI] 10.33696/pharmacol.3.028 PMC 바로가기 [Article Type] Article
Isolation and Characterization of Mouse Monoclonal Antibodies That Neutralize SARS-CoV-2 and Its Variants of Concern Alpha, Beta, Gamma and Delta by Binding Conformational Epitopes of Glycosylated RBD With High PotencyImmunology Published on 2021-10-262022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] B.1.1.7 B.1.167.2 B.1.351 Beta binding efficiency Blot Cell characterization characterized concern conformational contribute convalescent sera COVID-19 Delta Delta VOC Diagnosis Efficacy epitope epitopes expression Escherichia coli expressed flow cytometry full-length spike protein functional Gamma glycosylated HEK293T cell immunoprecipitation inhibit initial lentivirus mAb mAbs majority mammalian mice immunization monoclonal antibody mouse Mutation NAb neutralization Neutralizing activity neutralizing antibody neutralizing monoclonal antibodies P.1 Pathogenesis plaque-reduction neutralization test potency produced Protein pseudotyped RBD reduction reference sequence response rRBD SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD SARS-CoV-2 spike glycoprotein SARS-CoV-2 variant SARS-CoV-2 variants Spike protein suggested supernatant the RBD the SARS-CoV-2 therapy transmembrane Vaccine variants of concern VoC Wuhan [DOI] 10.3389/fimmu.2021.750386 PMC 바로가기 [Article Type] Immunology
Evaluation of Residual Infectivity after SARS-CoV-2 Aerosol Transmission in a Controlled Laboratory SettingArticle Published on 2021-10-242022-10-29 Journal: International Journal of Environmental Research an [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome aerosol Aerosols cell line clinical samples collected comparable Concentration condition conditions Contact Controlled coronavirus CPE cylindrical Cytopathic effect Dilution Efficiency in vitro Infectivity initial Laboratory molecular observation Particles post inoculation preventive measure quantification reported residual Residual infectivity Resilience Respiratory droplets RNA SARS-CoV-2 setting subject supernatant tested Transmission transmitted Transport Travel Vero E6 Viral load virus titer was determined was tested [DOI] 10.3390/ijerph182111172 PMC 바로가기 [Article Type] Article
Do We Really Need Hazard Prevention at the Expense of Safeguarding Death Dignity in COVID-19?Article Published on 2021-10-152022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] assumed Autopsy Cadaveric collected COVID-19 Cytopathic effect death Deceased defined demonstrated died dignity eight handling hazard hazards individual Infection involved literature review lung Nasopharyngeal swab Patient persist positive Post-mortem prevention replication capacity risk RT-PCR RT-PCR test Safeguarding SARS-CoV-2 SARS-COV-2 infection specimen subject supernatant supported tested tissue to define Transmission viral culture Viral transmission virus was collected WHO World Health Organization [DOI] 10.3390/diagnostics11101913 PMC 바로가기 [Article Type] Article
Mesenchymal Stem Cells in the Treatment of COVID-19, a Promising FutureReview Published on 2021-09-292022-10-31 Journal: Cells [Category] COVID-19, SARS, [키워드] acute respiratory distress Affect affected alterations ambient ARDS cardiac function cause Cell therapy cell types clinical trial COVID-19 COVID-19 patient culture media develop Diseases embryonic extracellular vesicle Factor Future genetic manipulation in vivo Inflammation Inflammatory response Intravenous injection lung Manifestations mesenchymal stem cell Mesenchymal stem cells MSC MSCs paracrine pluripotent stem cells renal Safe secretion Secretome STEM stem cell supernatant syndrome tested the disease therapeutic these cell Thromboembolism tissue tissue regeneration Treatment [DOI] 10.3390/cells10102588 PMC 바로가기 [Article Type] Review
Neutrophil Extracellular Traps Contribute to COVID-19 Hyperinflammation and Humoral Autoimmunity호중구 세포외 트랩은 COVID-19 과염증 및 체액성 자가면역에 기여합니다Article Published on 2021-09-262022-09-12 Journal: Cells [Category] SARS, 진단, [키워드] addressed antibody antinuclear antibody autoantibodies autoimmune responses complexes confocal microscopy contribute coronavirus disease correlated COVID-19 COVID-19 patient Critical cytokine cytokine level cytoplasmic Degradation DNA-complex DNase and autoimmunity ELISA evaluated flow cytometry granulocyte HMGB1 inducer inflammatory markers ISG-15 LDG LL-37 low-density macrophages NET NETs Neutrophils pro-inflammatory proportion Protein quantified SARS-CoV-2 secretion serum severe patients severity Structure supernatant was used [DOI] 10.3390/cells10102545 PMC 바로가기 [Article Type] Article