Generation of a Sleeping Beauty Transposon-Based Cellular System for Rapid and Sensitive Screening for Compounds and Cellular Factors Limiting SARS-CoV-2 ReplicationMicrobiology Published on 2021-07-292022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] A549 cells ACE2 acute respiratory disease acute respiratory syndrome Cell Cell culture cell line cellular Characteristics Compound Corona virus coronavirus COVID-19 CPE Cytopathic effect demonstrated Drug screening Efficiency eGFP experiment expression Factor Generation high throughput Host Infection infection model interferon response introduced labor-intensive live cell imaging lung mock-infected cell MONITOR overexpression post infection proliferation Rapid reporter restriction factor robust SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 susceptibility screened Screening sensitive sleeping beauty Spread Subclone System time-consuming TMPRSS2 viral replication [DOI] 10.3389/fmicb.2021.701198 PMC 바로가기 [Article Type] Microbiology
Novel N-Substituted 3-Aryl-4-(diethoxyphosphoryl)azetidin-2-ones as Antibiotic Enhancers and Antiviral Agents in Search for a Successful Treatment of Complex InfectionsArticle Published on 2021-07-272022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, 신종인플루엔자, [키워드] β-lactams 229E active against agent antibiotic antibiotic adjuvant Antiviral antiviral activity azetidin-2-one Cell cell line Compound coronavirus CPE cytostatic DNA and RNA Efficacy ENhance evaluated H1N1 subtype influenza A virus inhibitory activity MCC moderate molecular docking molecular dynamics Molecular dynamics simulation MRSA nine novel PBP2a phosphonates responsible selected Stereochemistry tested viruses [DOI] 10.3390/ijms22158032 PMC 바로가기 [Article Type] Article
Efficacy of “Essential Iodine Drops” against Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2)중증 급성 호흡기 증후군-코로나 바이러스 2 (SARS-COV-2)에 대한 "필수 요오드 방울"의 효능Research Article Published on 2021-07-092022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 치료제, [키워드] 1:1 aerosolization aqueous solution ATCC CCID Cell Cell culture complex controls coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patients CPE Cytopathic effect demonstrated Dilution drop effective end-point dilution Essential evaluated fulvic acid in vitro infecting infectious dose intranasal intranasally Iodine pandemic povidone-iodine Prevent quantified reduce reduced reducing reductions in respiratory respiratory droplet Respiratory droplets Result room temperature SARS-CoV-2 SARS-CoV-2 transmission SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome Spread temperature titre titres Vero Viral Viral load virucidal virus virus stock viruses was reduced was tested [DOI] 10.1371/journal.pone.0254341 PMC 바로가기 [Article Type] Research Article
Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assaysRT-PCR 진단 분석에 적합한 구아니듐 기반 운송 배지를 사용하여 타액에서 SARS-Cov-2 바이러스의 불 활성화Research Article Published on 2021-06-112022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 진단, 치료기술, [키워드] 1:1 abrogated buffer Cell culture chain Chain Reaction clinical sample clinical samples condition conditions Copan COVID-19 CPE Cytopathic effect detect detectable diagnostic Dilution downstream evaluated expand guanidinium hazard inactivated inactivation Incubation infectious limit of detection log media medium minute passages positive processing reaction remained respiratory respiratory sample Result room temperature RT-PCR Saliva sample collection SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA SARS-CoV-2 USA-WA1/2020 SARS-CoV-2 virus stability temperature tested Transport upper VeroE6 cell VeroE6 cells Viral Viral RNA virus [DOI] 10.1371/journal.pone.0252687 PMC 바로가기 [Article Type] Research Article
SARS-CoV2 infection impairs the metabolism and redox function of cellular glutathioneResearch Paper Published on 2021-06-102022-10-28 Journal: Redox Biology [Category] COVID-19, MERS, SARS, [키워드] Abstract Activation alteration Analysis Antiviral antiviral drug antiviral potency Cell cellular change COVID-19 CPE Cytopathic effect demonstrated ER stress expression data generate glutathione GSH GSSG homeostatic host cell hpi Image impair in vitro in vitro model in vitro study Increased infected cells Infection investigated marker membrane transporter metabolism NAC nelfinavir Nrf2 occurred oxidized glutathione precursor Prevent produced Protein Protein glutathionylation Proteins reduced Replication reported SARS-CoV-2 SARS-CoV2 SARS-CoV2 infection Schematic thiol Thiols transcription factor Treatment upregulation VERO E6 cells viral replication virus [DOI] 10.1016/j.redox.2021.102041 PMC 바로가기 [Article Type] Research Paper
Simple rapid in vitro screening method for SARS-CoV-2 anti-virals that identifies potential cytomorbidity-associated false positivesShort Report Published on 2021-06-092022-10-28 Journal: Virology Journal [Category] COVID-19, SARS, [키워드] activities Anti-viral anti-viral activity anti-viral drug antiviral drug arise assays candidate Cell Cell density cells cellular Concentration controls COVID-19 CPE Cytomorbidity Cytopathic effect cytotoxicity drug drug concentration Drug screening False positive False positives growth Hydroxychloroquine identify impairment in vitro include induce inhibit inhibit SARS-CoV-2 inhibited initial International methodology non-specific reduced Remdesivir replicate Replication Result ribavirin SARS-CoV-2 SARS-CoV-2 pandemic screening method simple staining Stress supplementary material Treatment Vero E6 cell viability virus [DOI] 10.1186/s12985-021-01587-z PMC 바로가기 [Article Type] Short Report
A Cytopathic Effect-Based Tissue Culture Method for HCoV-OC43 Titration Using TMPRSS2-Expressing VeroE6 CellsResearch Article Published on 2021-05-122022-10-30 Journal: mSphere [Category] Coronavirus, [키워드] 50% tissue culture infectious dose assay Accuracy antibody Cell cell entry cell lines cells complex contribute coronavirus costly CPE Culture culture supernatant Cytopathic effect early stage expressing HCoV-OC43 Human human coronavirus immunoperoxidase indicated infected cell Infection infectious dose IPA observation protease provide Research serine 2 staining substrates TCID time-consuming tissue titration TMPRSS2 transmembrane VeroE6 cell viral kinetics viral titer virus [DOI] 10.1128/mSphere.00159-21 PMC 바로가기 [Article Type] Research Article
Carbapenem-resistant bacteria in an intensive care unit during the coronavirus disease 2019 (COVID-19) pandemic: A multicenter before-and-after cross-sectional study코로나바이러스 2019(COVID-19) 대유행 중 중환자실의 카르바페넴 내성 박테리아: 다기관 전후 단면 연구Multicenter Study Published on 2021-04-162022-09-10 Journal: Infection Control and Hospital Epidemiology [Category] MERS, 치료제, [키워드] 95% confidence interval Acinetobacter activity antimicrobial resistance Bacteria carbapenem CIs clinical Cluster colonization conducted coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pandemic CPE cross-sectional cross-sectional study Enterobacteriaceae Genome sequencing horizontal transmission hospital ICU ICUs incidence indicate Infection infection control infection rate infection rates intensive care KPC Lineage Local LOESS mechanism multicenter pandemic Poisson regression rate ratio rate ratios Sequencing shift Spread strain Structure study period Transmission VIM Weekly [DOI] 10.1017/ice.2021.144 PMC 바로가기 [Article Type] Multicenter Study
Deep Transfer Learning Approach for Automatic Recognition of Drug Toxicity and Inhibition of SARS-CoV-2Article Published on 2021-04-022022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] accelerate acute respiratory syndrome Antiviral antiviral drug approach Automatic cause cell death Chloroquine Concentration Coronavirus-2 COVID-19 CPE Cytopathic effect deep deep learning Deep transfer learning drug Drug screening drugs DTL Emetine fine-tune Hydroxychloroquine Image implementation in vitro experiments include inhibition inhibitory effect learning Microscopy monolayer pandemic provided quantification Recognition Remdesivir responsible SARS-CoV-2 Screening TOX Toxicity transfer virus titer viruses [DOI] 10.3390/v13040610 PMC 바로가기 [Article Type] Article
Mining of high throughput screening database reveals AP-1 and autophagy pathways as potential targets for COVID-19 therapeutics고 처리량 스크리닝 데이터베이스의 마이닝은 COVID-19 치료제의 잠재적 표적으로서 AP-1 및 자가포식 경로를 나타냅니다Article Published on 2021-03-242022-09-10 Journal: Scientific Reports [Category] SARS, 신약개발, 유전자 메커니즘, 임상, 치료제, [키워드] addition Anti-SARS-CoV-2 Activity Antiviral AP-1 approved approved drug approved drugs autophagy autophagy pathway Candidates caused class coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 therapeutics CPE Cytopathic effect Data mining database drug drug activity drug library effective effort global pandemic High-throughput screening Hope house human clinical trials in vivo mechanism New coronavirus new coronavirus SARS-CoV-2 phenotypic Potential treatment potential treatments profile pursuit reveal SARS-CoV-2 SARS-COV-2 infection signaling pathway significantly target Target identification therapeutic Viral [DOI] 10.1038/s41598-021-86110-8 PMC 바로가기 [Article Type] Article