A Thermostable Oral SARS-CoV-2 Vaccine Induces Mucosal and Protective Immunity내열성 경구 SARS-CoV-2 백신은 점막 및 보호 면역을 유도합니다Article Published on 2022-02-252022-09-12 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] addition Administered administration antibody boost cellular Complete COVID-19 criteria Degradation effective expressing expression form Giardia lamblia hamsters IgA immune response induce injected intestinal intramuscularly Logistic membrane protein mice mucosal mucosal immunity Neutralizing oral oral vaccination platform pre-fusion preventing disease Protective Protein responses against robust SARS-CoV-2 spike surface protein the SARS-CoV-2 thermostable translate Trigger triggering Vaccination strategy Vaccine Vaccines viral challenge virus transmission Virus-like particle VLP VLP (virus-like particle) [DOI] 10.3389/fimmu.2022.837443 PMC 바로가기 [Article Type] Article
DNA Vaccines Expressing the Envelope and Membrane Proteins Provide Partial Protection Against SARS-CoV-2 in Mice엔벨로프 및 막 단백질을 발현하는 DNA 백신은 생쥐에서 SARS-CoV-2에 대한 부분적 보호를 제공합니다Article Published on 2022-02-242022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Against caused cellular Cellular immune response cellular response conserved coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cross-protective DNA DNA vaccine dose effective Electroporation elicited envelope envelope protein Epitopes humoral Humoral immunity Humoral response immune responses immunisation International Intramuscular injection lung tissue membrane membrane protein mice pandemic Partial protection Protein provided public health emergency respiratory robust SARS-CoV SARS-CoV-2 severe acute respiratory syndrome Coronavirus Significant structural protein structural proteins T-cell epitope target titre Vaccination strategies Vaccination strategy Vaccine vaccine candidate vaccine candidates Vaccines virus [DOI] 10.3389/fimmu.2022.827605 PMC 바로가기 [Article Type] Article
A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19코로나19 환자의 SARS-CoV-2 막관통 단백질에 대한 항체 반응을 평가하기 위해 유세포 분석과 결합된 세포 기반 시스템Article Published on 2022-02-222022-09-11 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, [키워드] antibody Antibody Response Antibody responses approach Biology Cell Biology Cell-based Assays Complete Effectiveness evaluate Flow flow cytometry Flow Cytometry/Mass Cytometry glycosylation Health Sciences HEK293T cell HEK293T cells host cells insertion Laboratory membrane membrane protein Microbiology Molecular biology N-glycosylation patient sera patients with COVID-19 powerful tool protocol SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants transmembrane protein Vaccine viral infection [DOI] 10.1016/j.xpro.2022.101229 PMC 바로가기 [Article Type] Article
Predicted 3D model of the M protein of Porcine Epidemic Diarrhea Virus and analysis of its immunogenic potentialResearch Article Published on 2022-02-092022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, [키워드] 3D model 3D structure activate Analysis antagonistic Antiviral B-cell epitopes binding cellular cellular mechanism epitope immunogenic potential induce initial linear B-cell epitope M protein membrane protein organized Pathways PEDV peptide predicted Protective Protective antibody protein interaction protocol strain T-cell epitope the SARS-CoV-2 transmembrane Treatment type I type III interferon Viral diseases viral envelope virus [DOI] 10.1371/journal.pone.0263582 PMC 바로가기 [Article Type] Research Article
Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2Article Published on 2022-02-072022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] acute respiratory syndrome approach bioprocess cGMP Characteristics characterization co-expression coronavirus coronavirus spike protein COVID-19 design of experiments different disease DoE downstream Downstream process ENhance experiment expression Gag generate genetic material good manufacturing practice HEK293 HIV-1 immunogenic membrane protein methodology optimization Particle performed platform Production Protein purification robust Safe SARS-CoV-2 SARS-CoV-2 VLP Structure transient transfection vaccine candidate Vaccine development variants virus VLP VLP vaccines VLPs wild-type [DOI] 10.3390/vaccines10020250 PMC 바로가기 [Article Type] Article
Photothermal inactivation of universal viral particles by localized surface plasmon resonance mediated heating filter membrane국소화 된 표면 플라즈몬 공명 매개 가열 필터 멤브레인에 의한 보편적 바이러스 입자의 광열 불 활성화Article Published on 2022-02-022022-09-01 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] 50% tissue culture infective dose cells Chain Reaction coated components Crystal violet Culture deformation denaturation dose effective gold H1Npdm09 HFM inactivation infectious sample infectious samples infective Infective dose Influenza virus irradiation Mardin membrane membrane protein Membrane proteins Nanoparticles Nanophotonics and plasmonics Natural hazards polymerase chain polymerase chain reaction provide qRT-PCR Quality control Quantitative reduce reduction reduction in reverse transcription room temperature SARS-CoV-2 staining surface plasmon surface plasmon resonance surface temperature TCID temperature tissue tissue culture Viral viral gene viral genes viral infectivity viral particle virus titer was reduced [DOI] 10.1038/s41598-022-05738-2 PMC 바로가기 [Article Type] Article
The E3 Ubiquitin Ligase RNF5 Facilitates SARS-CoV-2 Membrane Protein-Mediated Virion ReleaseResearch Article Published on 2022-02-012022-10-29 Journal: mBio [Category] COVID-19, MERS, [키워드] acute respiratory syndrome autophagy cellular membranes complex coronavirus dependent on E3 ligase ENhance enzyme Golgi apparatus Interaction Ligase maturation membrane protein Modification molecular mechanism performed regulate Release residue RNA interference RNF5 SARS-CoV-2 ubiquitin ubiquitination viral envelope protein viral particle virion virus virus-like particles [DOI] 10.1128/mbio.03168-21 PMC 바로가기 [Article Type] Research Article
Immunodominant and Neutralizing Linear B-Cell Epitopes Spanning the Spike and Membrane Proteins of Porcine Epidemic Diarrhea VirusImmunology Published on 2022-01-192022-11-01 Journal: Frontiers in Immunology [Category] Coronavirus, MERS, [키워드] amino acid sequence B-cell epitope cause Complete consensus sequence contribute demonstrated disease ELISA testing epidemic diarrhea epitope Epitope prediction genetic diversity greater group high mortality rate identify immune stimulation immunodominant epitope immunoinformatics insight linear linear B-cell epitope M protein membrane membrane protein NCBI database neutralization Neutralizing neutralizing epitope nine offer PEDV peptide peptide-based immunoassays Porcine epidemic diarrhea virus predicted profile Protein Proteins provided retrieved S2 subunit sera Seven Spike protein strain Strains the S protein the Spike Vaccine virus Virus neutralization [DOI] 10.3389/fimmu.2021.785293 PMC 바로가기 [Article Type] Immunology
SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles압타머 기능화된 금 나노입자를 이용한 SARS-CoV-2 검출Article Published on 2022-01-012022-09-11 Journal: Talanta [Category] SARS, 치료기술, [키워드] absorbance approach aptamer binding biosensing coagulant Concentration Containment Critical detect determine enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay examined Genome gold Gold nanoparticle inactivated light absorbance membrane protein MONITOR occur phosphate phosphate-buffered saline Protein protein concentration required SARS-CoV-2 SARS-CoV-2 detection spike Spike protein surface plasmon surface plasmon resonance the spike protein virus spread [DOI] 10.1016/j.talanta.2021.122841 PMC 바로가기 [Article Type] Article
Differential expression in humans of the viral entry receptor ACE2 compared with the short delta ACE2 isoform lacking SARS-CoV-2 binding sitesArticle Published on 2021-12-212022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] ACE2 ACE2 protein airway epithelia Amino acid antibody bind binding binding site cardiovascular system COVID-19 COVID-19 pandemic differential expression domain epithelia Evidence expression Extracellular full-length Health high affinity host cell Human human coronaviruses human tissue implicated Infection inter-individual isoform kidney likelihood liver lung Medical research membrane protein monomer predicted provide receptor receptor ACE2 reduced regulate SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 susceptibility sequence severity staining subsequent susceptibility the spike protein tissues Translational research Variation viral entry viral infection [DOI] 10.1038/s41598-021-03731-9 PMC 바로가기 [Article Type] Article