Structure-based virtual screening and molecular dynamics simulation of SARS-CoV-2 Guanine-N7 methyltransferase (nsp14) for identifying antiviral inhibitors against COVID-19COVID-19에 대한 항바이러스 억제제 식별을 위한 SARS-CoV-2 Guanine-N7 methyltransferase(nsp14)의 구조 기반 가상 스크리닝 및 분자 역학 시뮬레이션Article Published on 2021-08-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료제, [키워드] adenosine antiviral inhibitor antiviral inhibitors binding binding site C-terminal caused cellular immune responses Chinese Combination Compound Computational approaches conserved coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 therapeutics database domain drug target ensemble sampling enzyme evade Final genomic genomic RNA guanine Guanosine homology Host identify immune responses inhibiting inhibitor inhibitors Innate immunity interfere International involved main role MD simulations mechanism medical emergency Medicine Methylation methyltransferase molecular molecular dynamics Molecular dynamics simulation N7-MTase natural products nsp14 pandemic PCA performed protein targets provide researcher residue respiratory RNA RNA capping RNAs SARS-CoV SARS-CoV-2 screened Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 Specificities TCM the SARS-CoV-2 Traditional Traditional Chinese medicine unique viral genomic RNA Virtual screening [DOI] 10.1080/07391102.2020.1778535 PMC 바로가기 [Article Type] Article
Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 SubunitNSP10 서브 유닛에 대한 항체에 의해 밝혀진 SARS-COV-2 캡핑 효소의 국소화Article Published on 2021-07-292022-09-01 Journal: Viruses [Category] SARS, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibody capping capping enzyme causative agent Cell complex component coronavirus coronavirus disease Coronavirus disease-19 Coronavirus replication enzyme infected cell infected cells investigated Localization methyltransferase monoclonal antibody non-structural protein nsp10 nsp14 nsp16 pandemic protein complexes respiratory Revealed RNA RNA methyltransferase SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 subcellular localization subunit the SARS-CoV-2 [DOI] 10.3390/v13081487 PMC 바로가기 [Article Type] Article
N6-methyladenosine methyltransferases: functions, regulation, and clinical potentialReview Published on 2021-07-272022-10-29 Journal: Journal of Hematology & Oncology [Category] COVID-19, [키워드] Affect approach Cancer Chemotherapy complexes Drug discovery function functions highlight Immunotherapy m6A m6A methyltransferase methyltransferase Modification N6-methyladenosine Noncoding RNAs pathway Proteins Radiotherapy Regulation reported RNA RNAs stability targeted therapy therapy Therapy resistance Transcriptome translation widespread [DOI] 10.1186/s13045-021-01129-8 PMC 바로가기 [Article Type] Review
RBM15-mediated N6-methyladenosine modification affects COVID-19 severity by regulating the expression of multitarget genesRBM15 매개 N6-메틸아데노신 변형은 다중 표적 유전자의 발현을 조절하여 COVID-19 중증도에 영향을 미칩니다Article Published on 2021-07-232022-09-11 Journal: Cell Death & Disease [Category] 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Affect Alter Analysis biological processe Biomarkers cell death characterized coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 severity death disease severity elevated expression expression level expression levels Genes Host immune response in vitro Infection Infectious diseases Inflammatory response knockdown lymphocyte Lymphocytes lymphopenia mechanism methyltransferase Microarray Microarray analyses mild COVID-19 patients Modification modulated Multiorgan N6-methyladenosine performed positively correlated profile programmed cell death RBM15 reduced regulated respiratory SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severe and mild COVID-19 patients severe coronavirus disease severe patients significantly suppressed Symptom target Treatment treatment of COVID-19 [DOI] 10.1038/s41419-021-04012-z PMC 바로가기 [Article Type] Article
Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp14 RNA cap methyltransferaseBiochemical Techniques & Resources Published on 2021-07-162022-10-28 Journal: Biochemical Journal [Category] COVID-19, Fulltext, SARS, [키워드] activating Antiviral Antiviral compound can be used cofactor Compound coronavirus COVID-19 COVID-19 pandemic Critical Effect exhibited expressed fatal disease humans identify identifying immune system in vitro inhibitor investigated methyltransferase mRNA cap nsp14 performed Protein public health purified Remdesivir Research RNA SARS-CoV-2 SARS-COV-2 infection small molecule inhibitor synergistic the Coronaviridae the SARS-CoV-2 genome translation Treatment viral escape Viral protein viral replication Viral RNA [DOI] 10.1042/BCJ20210219 PMC 바로가기 [Article Type] Biochemical Techniques & Resources
Mn 2+ coordinates Cap-0-RNA to align substrates for efficient 2′- O -methyl transfer by SARS-CoV-2 nsp16MN 2+ SARS-COV-2 NSP16에 의한 효율적인 2'-O- 메틸 전달에 대한 기질을 정렬하는 CAP-0-RNA를 좌표Research Article Published on 2021-06-292022-09-10 Journal: Science Signaling [Category] MERS, SARS, 치료기술, [키워드] absence active site adenosylmethionine aligning Alter backbone binding biochemical capping catalysis coactivator complex conserved Coordinate coronavirus Coronaviruses Degradation Donor drug design efficient ENCODE enzyme Final generate heterodimeric Host host cell host defenses inhibitors innate response insert Interaction mammalian Messenger RNA metal ion metal ions methyl group methyltransferase mRNA nsp10 organism Prevent PROTECT resulting ribonucleotide ribonucleotides RNA RNAs SAM SARS-CoV-2 SARS-CoV-2 genome selective structural evidence Structure structures substrate substrates the SARS-CoV-2 transfer translation triggering unique Viral viral RNAs virus replication X-ray structure [DOI] 10.1126/scisignal.abh2071 PMC 바로가기 [Article Type] Research Article
Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 GenesArticle Published on 2021-05-312022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] antigen processing arising binding bioinformatics cell line Comparative complex complications of COVID-19 contribute COVID-19 pandemic Critical disease down-regulated down-regulated gene down-regulation Endoplasmic reticulum endoribonuclease (NSP15) enrichment analysis expression facilitate global public health heart development Heat shock Host host gene Human Immunity insulin signaling pathway Interaction methyltransferase Methyltransferase (NSP16) nervous system neurodegeneration neutrophil degranulation Nucleocapsid (NP) nucleocapsid protein offer oxidative phosphorylation pathway phosphatidylinositol 3-kinase positive Protein Regulation response RNA sequencing RNA-sequencing S1 subunit S2 subunit SARS-CoV-2 SARS-CoV-2 pathogenesis SARS-CoV-2 proteins SARS-CoV-2 virus Signaling signaling pathway spike Spike S1 Spike S2 Stress target host cell transcriptomic transcriptomic alteration up-regulated viral gene viral spike virus receptor [DOI] 10.3390/microorganisms9061193 PMC 바로가기 [Article Type] Article
2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography407 Published on 2021-05-252022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, SARS, [키워드] 5′-untranslated region acute respiratory syndrome CAP-1 complex conformational change coronavirus crystal structure Degradation Donor drug design drug target experiments Genome heterodimer Host inhibition Methylation methyltransferase Modification mRNA Nsp10/16 nucleases nucleotide performed Prevent PROTECT Protein reduce responsible RNA S-adenosyl methionine S-adenosylmethionine SARS-CoV-2 serial crystallography Significance Structure substrate temperature UTR viral proliferation Viral RNA [DOI] 10.1073/pnas.2100170118 PMC 바로가기 [Article Type] 407
Arginine methylation of SARS-Cov-2 nucleocapsid protein regulates RNA binding, its ability to suppress stress granule formation, and viral replicationSARS-Cov-2 뉴클레오캡시드 단백질의 아르기닌 메틸화는 RNA 결합, 스트레스 과립 형성 억제 능력 및 바이러스 복제를 조절합니다Research Article Published on 2021-05-232022-09-10 Journal: The Journal of Biological Chemistry [Category] SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 AP, affinity-purification arginine arginine methylation assembly binding blocked cancer treatment Cell culture clinical trial clinical trials component condensate coronavirus CTD, C-terminal dimerization domain defined DMSO, dimethylsulfoxide dose-dependent manner drugs FBS, fetal bovine serum Flag-N, Flag-epitope N protein G3BP1 genomic RNA GSK-3, glycogen synthase kinase 3 HEK293 cells hnRNPA1, heterogeneous nuclear ribonucleoprotein A1 host antiviral response host protein inhibited inhibiting inhibitor inhibitors Interaction life cycle lysine MERS-CoV, Middle East respiratory syndrome coronavirus methylarginine methylated Methylation methyltransferase motifs N protein N, nucleocapsid NTD, N-terminal RNA-binding domain nucleocapsid (N) protein nucleocapsid protein PAR-CLIP, photoactivatable ribonucleoside–enhanced crosslinking and immunoprecipitation PFA, paraformaldehyde PRMT1 PRMTs, protein arginine methyltransferases property Protein protein 3 RBP, RNA binding protein reduced regulate regulated required residue residues respiratory RGG/RG motif RIP, RNA immunoprecipitation RNA RNA binding S, spike SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 replication SARS-CoV-2 virion SARS-CoV-2 virions SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 sequences severe acute respiratory syndrome Coronavirus SGs, stress granules significantly SMN, survival of motor neuron SR, serine/arginine-rich SRPK, SRSF protein kinase Stage Stress stress granule stress granules Substitution substrates suppress TDRD3, Tudor domain-containing protein 3 the N protein therapeutic Treatment Type type I type I PRMT inhibitor VeroE6 cell VeroE6 cells Viral viral genome viral life cycle Viral protein Viral proteins viral replication virion virions vRNP, viral ribonucleoprotein [DOI] 10.1016/j.jbc.2021.100821 PMC 바로가기 [Article Type] Research Article
METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infectionMETTL3은 SARS-CoV-2 감염 동안 바이러스 m6A RNA 변형 및 숙주 세포 선천성 면역 반응을 조절합니다Article Published on 2021-05-112022-09-11 Journal: Cell Reports [Category] SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus adenosine binding clade coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pathogenesis COVID-19 treatment decrease disease downstream ENhance expression Gene Expression Host host cell host genes Host immune response host innate immunity increase Inflammation Inflammatory inflammatory gene inflammatory genes innate immune innate immune response Innate immunity m(6)A RNA modification. methyltransferase METTL3 Modification molecular mechanism Patient physiological reduced reduction in regulate RIG-I RNA RNA modification SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus severe coronavirus disease signaling pathway the SARS-CoV-2 the SARS-CoV-2 virus Treatment viral genome Viral RNA widespread [DOI] 10.1016/j.celrep.2021.109091 PMC 바로가기 [Article Type] Article