Probing the SAM Binding Site of SARS-CoV-2 Nsp14 In Vitro Using SAM Competitive Inhibitors Guides Developing Selective Bisubstrate InhibitorsSAM 경쟁 억제제를 사용한 시험관 내 SARS-CoV-2 Nsp14의 SAM 결합 부위 조사 선택적 이중 기질 억제제 개발 가이드Article Published on 2021-10-012022-09-11 Journal: SLAS discovery : advancing life sciences R & D [Category] SARS, 유전자 메커니즘, 치료제, [키워드] adenosylmethionine Antiviral binding cause competitive inhibitor Consequences coronavirus COVID-19 COVID-19 pandemic Developing DNA evade Guanosine guide Healthcare system Healthcare systems house Human immune system inhibited inhibitor library lysine mechanism of action Methylation methyltransferase NSP nsp14 Nsps optimization other coronaviruses path Probing Protein provided RNA SAM SARS-CoV-2 SARS-CoV-2 virus selective significant difference significant differences Site the disease these compound these compounds Viral RNA [DOI] 10.1177/24725552211026261 PMC 바로가기 [Article Type] Article
An atomistic model of the coronavirus replication-transcription complex as a hexamer assembled around nsp15Research Article Published on 2021-09-232022-10-30 Journal: The Journal of Biological Chemistry [Category] COVID-19, MERS, SARS, [키워드] 5′-UTR Applying binding complex coronavirus CTD, C-terminal domain docking dsRNA EndoN, endonuclease exonuclease function generate GTase, guanylyltransferase indicate involved MD, molecular dynamics MHV, murine hepatitis virus molecular Molecular modeling mRNA mRNA transcripts MST, microscale thermophoresis MTase, methyltransferase N, nucleocapsid nsp, nonstructural viral protein nsp13 nsp14 Nsp15 NTD, N-terminal domain NTPase, nucleoside triphosphatase nucleocapsid polymerase Proofreading Protein protein structure question recruit Replication replication-transcription complex responsible RNA RTC, replication-transcription complex SARS-CoV-2 Structure structure model subunit switching Transcription trimer TRS-B, body TRS TRS-L TRS-L, leader TRS TRS, transcription regulatory sequence viral genome Viral protein viral replication Viral RNA viral transcription while [DOI] 10.1016/j.jbc.2021.101218 PMC 바로가기 [Article Type] Research Article
New targets for drug design: importance of nsp14/nsp10 complex formation for the 3′-5′ exoribonucleolytic activity on SARS-CoV-2약물 설계를 위한 새로운 표적: SARS-CoV-2에 대한 3'-5' 엑소리보핵분해 활성에 대한 nsp14/nsp10 복합체 형성의 중요성Article Published on 2021-09-012022-09-11 Journal: The Febs Journal [Category] SARS, 신약개발, 치료제, [키워드] activities activity Amino acid amino acids antagonist antiviral drugs assembly biochemical C-terminal catalytic complex Consequences conserved coronavirus Coronaviruses Critical DEDD disrupt domain ExoN extreme global pandemic implication in vitro in vitro activity independent independent of inhibitor inhibitors Interaction interfere interferon involved life cycle mapping molecular Molecular modeling motif MTase multifunctional protein N-terminal New nsp10 nsp14 other coronaviruses pathogenesis of SARS-CoV-2 performed Protein Replication required residue RNase SARS-CoV-2 SARS-CoV-2 virus stimulated target triggered upregulating virus [DOI] 10.1111/febs.15815 PMC 바로가기 [Article Type] Article
Reconstitution and functional characterization of SARS-CoV-2 proofreading complexSARS-CoV-2 교정복합체의 재구성 및 기능적 특성화Article Published on 2021-09-012022-09-11 Journal: Protein expression and purification [Category] SARS, 치료법, [키워드] activated acute respiratory syndrome coronavirus base pair base pairs characterized complex coronavirus 2 COVID-19 double-stranded RNA function functional functions in vitro inhibited metabolism mismatch nsp10 nsp14 NSP8 Nuclease pandemic primer Proofreading purified Replication replication of SARS-CoV-2 replication-transcription complex respiratory RNA RNA genome RNA replication RNAs RNase RTC SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA severe acute respiratory syndrome coronavirus 2 Structure structures the SARS-CoV-2 while [DOI] 10.1016/j.pep.2021.105894 PMC 바로가기 [Article Type] Article
Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-MethyltransferasemRNA 캡 구아닌 N7-메틸트랜스퍼라제를 표적으로 하는 잠재적인 SARS-CoV-2 항바이러스제의 식별 및 평가Article Published on 2021-09-012022-09-11 Journal: Antiviral Research [Category] SARS, 치료제, [키워드] analyzed antiviral activity antiviral agent approach assays binding binding site Cell Compound COVID-19 COVID-19 pandemic dot blot Drug discovery ENCODE enzyme FIVE Fluorescence fluorescence anisotropy Fluorescence assay guanine HPLC identification identify inhibitor Inhibitor screening inhibitory effect Inhibitory effects insight involved mRNA novel nsp14 SARS-CoV-2 screened Structure tested therapeutic intervention therapeutic interventions to define [DOI] 10.1016/j.antiviral.2021.105142 PMC 바로가기 [Article Type] Article
Live attenuated coronavirus vaccines deficient in N7-Methyltransferase activity induce both humoral and cellular immune responses in miceN7-메틸트랜스퍼라제 활성이 결핍된 약독화 코로나바이러스 생백신은 마우스에서 체액성 및 세포성 면역 반응을 모두 유도합니다Original Article Published on 2021-08-182022-09-10 Journal: Emerging Microbes & Infections [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antiviral approach Attenuated vaccines cap structure caused CD4 CD8 Cell culture Cellular immune response conserved coronavirus coronavirus vaccine Coronavirus vaccines Coronaviruses CoV COVID-19 pandemic CoVs Economy effective function guanine Health hosts humans humoral humoral immune response immune responses immunization induce infect Infection involved Live attenuated vaccine Live attenuated vaccines livestock MHV MHV-A59 mice murine hepatitis virus mutant N7-Methyltransferase nsp14 Protein provide replication efficiency respiratory RNA RNA virus robust SARS-CoV-2 severe acute respiratory syndrome Coronavirus single dose T cell T cell responses the cell Treatment Vaccine variety Viral Viral RNA virus viruses wild-type [DOI] 10.1080/22221751.2021.1964385 PMC 바로가기 [Article Type] Original Article
Structure-based virtual screening and molecular dynamics simulation of SARS-CoV-2 Guanine-N7 methyltransferase (nsp14) for identifying antiviral inhibitors against COVID-19COVID-19에 대한 항바이러스 억제제 식별을 위한 SARS-CoV-2 Guanine-N7 methyltransferase(nsp14)의 구조 기반 가상 스크리닝 및 분자 역학 시뮬레이션Article Published on 2021-08-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료제, [키워드] adenosine antiviral inhibitor antiviral inhibitors binding binding site C-terminal caused cellular immune responses Chinese Combination Compound Computational approaches conserved coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 therapeutics database domain drug target ensemble sampling enzyme evade Final genomic genomic RNA guanine Guanosine homology Host identify immune responses inhibiting inhibitor inhibitors Innate immunity interfere International involved main role MD simulations mechanism medical emergency Medicine Methylation methyltransferase molecular molecular dynamics Molecular dynamics simulation N7-MTase natural products nsp14 pandemic PCA performed protein targets provide researcher residue respiratory RNA RNA capping RNAs SARS-CoV SARS-CoV-2 screened Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 Specificities TCM the SARS-CoV-2 Traditional Traditional Chinese medicine unique viral genomic RNA Virtual screening [DOI] 10.1080/07391102.2020.1778535 PMC 바로가기 [Article Type] Article
Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 SubunitNSP10 서브 유닛에 대한 항체에 의해 밝혀진 SARS-COV-2 캡핑 효소의 국소화Article Published on 2021-07-292022-09-01 Journal: Viruses [Category] SARS, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibody capping capping enzyme causative agent Cell complex component coronavirus coronavirus disease Coronavirus disease-19 Coronavirus replication enzyme infected cell infected cells investigated Localization methyltransferase monoclonal antibody non-structural protein nsp10 nsp14 nsp16 pandemic protein complexes respiratory Revealed RNA RNA methyltransferase SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 subcellular localization subunit the SARS-CoV-2 [DOI] 10.3390/v13081487 PMC 바로가기 [Article Type] Article
Combining SARS-CoV-2 Proofreading Exonuclease and RNA-Dependent RNA Polymerase Inhibitors as a Strategy to Combat COVID-19: A High-Throughput in silico ScreeningMicrobiology Published on 2021-07-202022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] absence acute respiratory syndrome Antiviral antiviral activities approach approved drug AutoDock binding free energy clinical settings clinical trial combat combinatorial therapy coronavirus COVID-19 curative treatment Dexamethasone docking domain drug Drug repurposing effective Effectiveness ExoN proofreading exonuclease exonuclease inhibitor identify in silico in vitro inhibitor inhibitors Interaction MD simulation molecular molecular docking molecular docking and MD simulation nsp14 nucleoside nucleoside analog Prophylactic RdRP RdRp inhibitor RNA RNA-dependent RNA polymerase SARS-CoV-2 screened Screening shown solvent Strategy the disease therapy treat Treatment [DOI] 10.3389/fmicb.2021.647693 PMC 바로가기 [Article Type] Microbiology
Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp14 RNA cap methyltransferaseBiochemical Techniques & Resources Published on 2021-07-162022-10-28 Journal: Biochemical Journal [Category] COVID-19, Fulltext, SARS, [키워드] activating Antiviral Antiviral compound can be used cofactor Compound coronavirus COVID-19 COVID-19 pandemic Critical Effect exhibited expressed fatal disease humans identify identifying immune system in vitro inhibitor investigated methyltransferase mRNA cap nsp14 performed Protein public health purified Remdesivir Research RNA SARS-CoV-2 SARS-COV-2 infection small molecule inhibitor synergistic the Coronaviridae the SARS-CoV-2 genome translation Treatment viral escape Viral protein viral replication Viral RNA [DOI] 10.1042/BCJ20210219 PMC 바로가기 [Article Type] Biochemical Techniques & Resources