Development of SARS-CoV2 humoral response including neutralizing antibodies is not sufficient to protect patients against fatal infection중화 항체를 포함한 SARS-COV2 체액 반응의 발달은 치명적인 감염으로부터 환자를 보호하기에 충분하지 않습니다.Clinical Trial Published on 2022-02-082022-09-01 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 변종, 비임상, 유전자 메커니즘, 임상, 진단, 치료기술, [키워드] animal models anti-SARS-CoV-2 antibodies anti-SARS-CoV-2 antibody anti-SARS-CoV-2 IgG antibodies antibody Antibody Response asymptomatic individual Asymptomatic individuals asymptomatic patients breath characterized clearance COVID-19 COVID-19 patients Deceased development died disease severity form health emergency house humoral Humoral response IgA IgM immune response Immunological disorders in viral in-house ELISA Infection management modulation Neutralization assay Neutralizing antibodies neutralizing antibody pandemic passive transfer Patient patients persistence plasma PROTECT question recovered patient recovered patients Region reported SARS-CoV-2 SARS-COV-2 infection SARS-CoV2 serum severe COVID-19 specific antibodies the antibody response the disease the RBD titers titers of IgG transfer Vaccine design Viral viral clearance virus-specific antibodies while [DOI] 10.1038/s41598-022-06038-5 PMC 바로가기 [Article Type] Clinical Trial
A fast and cheap in-house magnetic bead RNA extraction method for COVID-19 diagnosis코로나19 진단을 위한 빠르고 저렴한 자체 자기 비드 RNA 추출 방법Article Published on 2022-02-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 치료기술, [키워드] addition affecting automated COVID-19 COVID-19 diagnosis COVID-19 test cycle extraction FIVE genetic material gold gold standard high correlation house magnetic bead Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nasopharyngeal swabs nucleic acid nucleic acids pandemic platform positive protocol public health purification qPCR quantitative PCR Rapid reagents reduced Reverse transcription quantitative PCR RNA RNA extraction RNA extraction kits RT-qPCR SARS-CoV-2 SARS-CoV-2 detection the mean virus detection [DOI] 10.1016/j.jviromet.2021.114414 PMC 바로가기 [Article Type] Article
Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay상업용 이중 표적 qRT-PCR 진단 분석에서 SARS-CoV-2 ORF1ab의 증폭 감소Article Published on 2022-02-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (RdRp) amplification Cobas Ct-value Ct-values diagnostic diagnostics diminished discrepancy Dual-target assay E-gene false-negative false-negative results genomic region highlight house limit molecular molecular diagnostics Molecular diagnostics SARS-CoV-2 nsp10 nucleotide nucleotide mutation ORF ORF1 ORF1ab ORF1ab mutations PCR PCR amplification primer probe qRT-PCR RdRP resulting SARS-CoV-2 SARS-CoV-2 PCR tests SARS-CoV-2 variant Surveillance target region target regions unique [DOI] 10.1016/j.jviromet.2021.114397 PMC 바로가기 [Article Type] Article
Surveillance Web System and Mouthwash-Saliva qPCR for Labor Ambulatory SARS-CoV-2 Detection and Prevention분만 이동 SARS-CoV-2 감지 및 예방을 위한 감시 웹 시스템 및 구강 세척제-타액 qPCRArticle Published on 2022-01-242022-09-10 Journal: International Journal of Environmental Research an [Category] SARS, 변종, 치료기술, 치료제, [키워드] Algorithm Alpha Alpha variant ambulatory applied Asymptomatic baseline Community conducted copy number coronavirus COVID-19 database Decision-making detection Detection limit development effective epidemiological epidemiological surveillance Evidence evidence of Follow-up house individual labor Laboratory Mild moderate monitoring Mouthwash N-gene nasopharyngeal swabbing platform populations prevention primer Primers protocol provide provided qPCR RT-qPCR RT-qPCR test Safe SARS-CoV-2 SARS-CoV-2 sequence SARS-CoV-2 sequences SARS-CoV-2 testing sensitivity specificity specimen statistical Surveillance symptomatic symptomatic case System threshold Viral virus virus inactivation volunteers Web work environments [DOI] 10.3390/ijerph19031271 PMC 바로가기 [Article Type] Article
An international, interlaboratory ring trial confirms the feasibility of an extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples국제적, 간사 링 시험은 임상 샘플에서 SARS-COV-2 RNA의 신뢰할 수있는 검출을위한 추출없는 "직접"RT-QPCR 방법의 타당성을 확인합니다.Research Article Published on 2022-01-132022-09-10 Journal: PLoS ONE [Category] 유전자 메커니즘, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Aliquots applied Chain Reaction charcoal clinical sample clinical samples collected Concordance coronavirus coronavirus disease COVID-19 Cycle threshold values equipment extraction feasibility feasible generate guanidine house inhibit International Laboratory limitation nasopharyngeal Nasopharyngeal samples nucleic acid nucleic acids pandemic Patient PCR reaction performed pharyngeal polymerase chain polymerase chain reaction positive protocol provided purified quantitative polymerase chain reaction reagents resource respiratory reverse transcription RT-PCR RT-PCR assay RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA severe acute respiratory syndrome Coronavirus Spread Transcription translated Trial Viral viral transport media [DOI] 10.1371/journal.pone.0261853 PMC 바로가기 [Article Type] Research Article
Prevalence of SARS-Cov-2 antibodies and living conditions: the French national random population-based EPICOV cohortSARS-COV-2 항체 및 생활 조건의 유병률 : 프랑스 국가 임의 인구 기반 Epicov 코호트Research Published on 2022-01-092022-09-01 Journal: BMC Infectious Diseases [Category] SARS, 진단, 치료기술, [키워드] accounted Adjustment affected age antibody assays children Cohort collected condition Coverage COVID-19 COVID-19 case detect dried blood spot Dried blood spots eligible epidemic wave Euroimmun Europe France French healthcare home house household household size identify IgG antibodies IgG antibody Immigrants individual initial Mask Masks Migration National Neutralizing antibodies neutralizing antibody participant Population Population-based survey questionnaire random Random sample random selection reported Result Risk factors SARS-CoV-2 SARS-COV-2 infection Seropositivity Seroprevalence seroprevalence survey smoking Spike protein spread of COVID-19 supplementary material telephone tested Univariate analysis virological test virological tests was performed [DOI] 10.1186/s12879-021-06973-0 PMC 바로가기 [Article Type] Research
Comparative performance of WANTAI ELISA for total immunoglobulin to receptor binding protein and an ELISA for IgG to spike protein in detecting SARS-CoV-2 antibodies in Kenyan populations케냐 인구에서 SARS-CoV-2 항체를 검출할 때 수용체 결합 단백질에 대한 총 면역글로불린에 대한 WANTAI ELISA 및 스파이크 단백질에 대한 IgG에 대한 ELISA의 비교 성능Article Published on 2022-01-012022-09-10 Journal: Journal of Clinical Virology [Category] SARS, 진단, 치료기술, [키워드] 95% CI approved assays blood donor Blood donors Characteristics Comparative COVID-19 test cross-sectional survey described detect ELISA gold gold standard house IgG immunoassay Immunoglobulin individuals malaria overall concordance Population positive positive sample Protein Receptor binding SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 PCR sensitivity serology seronegative seropositive Seroprevalence specificity Spike protein the WHO Total immunoglobulin variety widespread [DOI] 10.1016/j.jcv.2021.105061 PMC 바로가기 [Article Type] Article
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2SARS-CoV-2의 N501Y 변이체를 신속하게 식별하기 위한 저비용 TaqMan 작은 홈 결합제 프로브 기반 1단계 RT-qPCR 분석Article Published on 2022-01-012022-09-11 Journal: Journal of virological methods [Category] SARS, 변종, 치료기술, [키워드] Accuracy allele frequency analytical sensitivity B.1.1.7 B.1.351 collected COVID-19 detect determine diagnostic Diagnostic accuracy evaluated expand Frequency Genome Genome sequencing gold gold standard health authorities house identify in some Lineage low frequency Mutation N501Y N501Y mutation N501Y variants One-step RT-qPCR positive Prevalence qPCR qPCR assay reduced reference standard respiratory specimen respiratory specimens robust RT-qPCR SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 variant SARS-CoV-2 variants Sequencing Surveillance TaqMan time-consuming Transmissibility variant variant of concern variants variants of concern variants of SARS-CoV-2 whole-genome sequencing widespread wildtype [DOI] 10.1016/j.jviromet.2021.114333 PMC 바로가기 [Article Type] Article
Development of SARS-CoV-2 packaged RNA reference material for nucleic acid testing핵산 검사를위한 SARS-COV-2 패키지 RNA 기준 재료의 개발Paper in Forefront Published on 2021-12-272022-09-01 Journal: Analytical and bioanalytical chemistry [Category] COVID19(2023년), Fulltext, MERS, SARS, 유전자 메커니즘, 진단, 치료기술, 치료법, 치료제, [키워드] assays can be used Concentration copy number COVID-19 pandemic detect development diagnostics digital PCR extracted RNA Genes Genome homogeneity house IMPROVE Laboratory lentiviral Lentiviral particle molecular Molecular diagnosis nucleic acid nucleic acid tests packaged performed RdRP Reference material (RM) Reference materials reference values reliability required reverse transcription RNA SARS-CoV-2 SARS-CoV-2 virus stability supplementary material target target gene Target genes the SARS-CoV-2 virus undergo Viral was measured was used [DOI] 10.1007/s00216-021-03846-y PMC 바로가기 [Article Type] Paper in Forefront
Evaluation of Three Quantitative Anti-SARS-CoV-2 Antibody Immunoassays3가지 정량적 항-SARS-CoV-2 항체 면역분석법의 평가Research Article Published on 2021-12-222022-09-10 Journal: Microbiology Spectrum [Category] SARS, 진단, 치료기술, [키워드] (Abbott Abbott acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 anti-SARS-CoV-2 anti-SARS-CoV-2 antibodies anti-SARS-CoV-2 antibody antibody Antibody titer Antibody titers Antigen binding antibodies caused Chemiluminescent assay convalescent plasma coronavirus correlated correlation COVID Critical detect determine DiaSorin ELISA evaluated herd immunity house identify IgG Immunity immunization immunoassay Infection International international standard less LIAISON Live virus monoclonal antibodies Neutralization assay Neutralizing antibodies Neutralizing antibody titer neutralizing antibody titers NIBSC not correlated pandemic performed Postinfection proportional bias Protective respiratory SARS-CoV-2 SARS-CoV-2 IgG SARS-CoV-2 strain sensitivities sensitivity sera serological Serological assay serological assays serological result serological results severe acute respiratory syndrome Coronavirus significantly Specificities specificity spike tested the immunoassay the SARS-CoV-2 Treatment vaccination virus neutralization assay virus neutralization assays Virus spike WHO [DOI] 10.1128/spectrum.01376-21 PMC 바로가기 [Article Type] Research Article