Variable levels of spike and ORF1ab RNA in post-mortem lung samples of SARS-CoV-2-positive subjects: comparison between ISH and RT-PCRSARS-CoV-2 양성 대상자의 사후 폐 샘플에서 다양한 수준의 스파이크 및 ORF1ab RNA: ISH와 RT-PCR 간의 비교Original Article Published on 2022-02-012022-09-10 Journal: Virchows Archiv [Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제, [키워드] amplification amplification cycle amplification cycles analyzed cellular Chain Reaction correlation Deceased demonstrated detect E gene electron microscopy envelope FFPE formalin formalin-fixed paraffin-embedded Frame gene amplification IHC immunohistochemistry In situ hybridization In situ hybridization (ISH) Microscopy morphology N gene nucleocapsid Open reading frame Open reading frame (ORF1ab) optimization ORF1 ORF1ab ORF1ab gene paraffin polymerase chain polymerase chain reaction positive Post-mortem post-mortem lung Post-mortem lung samples reverse transcription reverse transcription polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) RNA RT-PCR S gene SARS-CoV-2 sensitivity sequence Spike(S) staining subjects supplementary material the SARS-CoV-2 tissue variable Viral Viral RNA virus virus detection [DOI] 10.1007/s00428-021-03262-8 PMC 바로가기 [Article Type] Original Article
Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages혈통의 지표로서 SARS-CoV-2 유전자 변이를 검출하기 위한 상용 RT-qPCR 분석의 유용성Article Published on 2022-02-012022-09-11 Journal: Journal of virological methods [Category] Coronavirus, COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] affected B.1.1.7 B.1.177 changes in circulating variant circulating variants community transmission concerning cycle threshold described detect Diagnosis evaluated Health Organization Infection Lineage lineages molecular Mutation N gene PCR RdRP RdRp gene reduce RT-qPCR RT-qPCR test S gene SARS-CoV-2 SARS-CoV-2 testing SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2 virus Spread Surveillance target region the SARS-CoV-2 the SARS-CoV-2 virus variants Variation variations was performed WHO World Health Organization [DOI] 10.1016/j.jviromet.2021.114428 PMC 바로가기 [Article Type] Article
Early assessment of the clinical severity of the SARS-CoV-2 omicron variant in South Africa: a data linkage study남아프리카 공화국에서 SARS-CoV-2 오미크론 변이체의 임상 중증도에 대한 조기 평가: 데이터 연계 연구Articles Published on 2022-01-292022-09-10 Journal: Lancet (London, England) [Category] 변종, [키워드] 95% CI adjusted odds ratio Admission African Analysis center Clinical severity Control COVID-19 COVID-19 case COVID-19 cases COVID-19 PCR test delta variant Department diagnosed diagnosed with COVID-19 disease control disease severity Factor finding foundation funding Genome sequencing Hospital admission hospitalisation hospitalisations hospitalised Immunity increase in individual infected individuals Infection infections Laboratory Laboratory test Medical Research Council Medicine Melinda Gate Melinda Gates multivariable logistic regression multivariable logistic regression models National Odds ratio Omicron variant PCR test PCR tests prevention proportion reduced reported S gene S gene target failure SARS-CoV-2 SARS-CoV-2 genome severe disease severity significantly significantly lower Society South South Africa TaqPath the SARS-CoV-2 Thermo Fisher Scientific variant Wellcome Trust [DOI] 10.1016/S0140-6736(22)00017-4 PMC 바로가기 [Article Type] Articles
Increased risk of infection with SARS-CoV-2 Omicron BA.1 compared with Delta in vaccinated and previously infected individuals, the Netherlands, 22 November 2021 to 19 January 20222021년 11월 22일부터 2022년 1월 19일까지 예방 접종 및 이전에 감염된 개인의 SARS-CoV-2 Omicron BA.1 감염 위험 증가Rapid Communication Published on 2022-01-272022-09-10 Journal: Eurosurveillance [Category] SARS, 변종, [키워드] 95% confidence interval air-borne infections Delta delta variant effective Epidemiology Immunity Increased increased risk infected individual infected individuals Laboratory less naïve individuals Netherlands Odds ratio omicron Omicron variant Predictive risk of infection S gene S gene target failure SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 Omicron Variant of Concern Vaccine escape SARS-CoV-2 variant SARS-CoV-2-infected individual severe acute respiratory syndrome - SARS Vaccine vaccines and immunisation variant viral infections [DOI] 10.2807/1560-7917.ES.2022.27.4.2101196 PMC 바로가기 [Article Type] Rapid Communication
Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencingSanger 시퀀싱에 의한 SARS-COV-2 스파이크 유전자의 게놈 감시Lab Protocol Published on 2022-01-202022-09-10 Journal: PLoS ONE [Category] 변종, 진단, 치료기술, [키워드] adopted Complete COVID effective evade Evolution evolutionary dynamics feasible Genomic surveillance groups Laboratory measure methodology monitoring Mutation nucleotide pandemic polymorphism protocol reduction responsible resulting reveal S gene SARS-CoV-2 SARS-CoV-2 S gene Sequencing spike spike gene Support Surveillance vaccine immunity variant variants [DOI] 10.1371/journal.pone.0262170 PMC 바로가기 [Article Type] Lab Protocol
SARS-COV-2 Variants: Differences and Potential of Immune EvasionSARS-COV-2 변종: 면역 회피의 차이점과 가능성Review Published on 2022-01-182022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 변종, 진단, [키워드] ACE-2 receptor acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 addressed B.1.1.529 B.1.1.7 B.1.351 B.1.617.2 Brazil Characteristics concerning Coronavirus-2 COVID-19 COVID-19 pandemic Critical critical points delta variant difference disease severity generate glycoprotein immune Immune escape IMPROVE include India Infection Mutation Neutralizing antibodies neutralizing antibody Omicron variant P.1 pandemic Perspective Potential potential risk reducing Reinfection resistance to neutralizing antibodies respiratory risk S gene SARS-CoV-2 selective Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 South Africa survival the SARS-CoV-2 Transmissibility vaccination Vaccines variant variant of concern variants variants of concern Viral viral fitness viral replication VoC VOCs [DOI] 10.3389/fcimb.2021.781429 PMC 바로가기 [Article Type] Review
Molecular Characterization of Severe Acute Respiratory Syndrome Coronavirus 2 Isolates From Central Inner SardiniaMicrobiology Published on 2022-01-142022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Amplicon Analysis approach characterization characterized circulation Cluster conserved coronavirus 2 COVID-19 distribution effort Epidemiology Evolution Frequency Genes genetic diversity Genome sequencing geographical location incidence increasingly Infection Inner involved isolate Italy Lineage mapping Measures Missense molecular characterization MONITOR mutated Mutation mutation rate N gene next generation sequencing Nucleocapsid proteins nucleotide nucleotides pandemic pandemic (COVID-19) Phylogeny Prevalence Public health intervention RdRP respiratory respiratory sample Result S gene Sardinia Sardinia—Italy SARS-CoV-2 SARS-CoV-2 infected patient SARS-CoV-2 pandemic sequence Sequencing stimulated subsequent synonymous synonymous variant the spike protein transmission clusters Variability variant viral genome widespread [DOI] 10.3389/fmicb.2021.827799 PMC 바로가기 [Article Type] Microbiology
SARS-CoV-2 variants with reduced infectivity and varied sensitivity to the BNT162b2 vaccine are developed during the course of infection감염성이 감소하고 BNT162B2 백신에 대한 감도가 다양한 SARS-COV-2 변이체가 감염 과정에서 개발됩니다.Clinical Trial Published on 2022-01-122022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, 변종, 임상, 치료기술, 치료법, [키워드] addition Affect Analysis analyzed Anti-spike Antibody neutralization antibody neutralization activity Author BNT162b2 BNT162b2 vaccine circulating convalescent plasma Course COVID-19 vaccine COVID-19 vaccines demonstrated elicit employment Evolution Frequency highlight Host human host human population impair in-depth analysis increase in individual infected individual infected individuals Infection infectivity capacity intra-host SARS-CoV-2 variant intra-host SARS-CoV-2 variants Intra-host variant intra-host variants L1197I mutation landscape less low frequencies mRNA mRNA-based mutated mutated variant mutated variants N gene N genes natural infection neutralization Neutralizing antibodies nose-throat swab nose-throat swabs pandemic plasma plasma neutralization activity platform Point mutation potential circulating variants Protective Quasispecies quasispecy raised reduced respiratory Respiratory system S gene S2 subunit SARS-CoV-2 SARS-CoV-2 evolution SARS-CoV-2 intra-host variability SARS-CoV-2 intra-host variant SARS-CoV-2 intra-host variants SARS-CoV-2 mutagenesis SARS-CoV-2 variant SARS-CoV-2 variants sensitivity Sequencing Sequencing database Sequencing databases single-genome sequencing Spike protein Spike proteins spike variant Spread swab sample swab samples the SARS-CoV-2 the spike protein vaccinated individuals vaccination rate variant variants variants of concern [DOI] 10.1371/journal.ppat.1010242 PMC 바로가기 [Article Type] Clinical Trial
Discriminatory Weight of SNPs in Spike SARS-CoV-2 Variants: A Technically Rapid, Unambiguous, and Bioinformatically Validated Laboratory ApproachArticle Published on 2022-01-112022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] Alpha variant analyses Analysis assigned assumed Beta bioinformatic validation COVID-19 COVID-19 pandemic COVID-19 variants demonstrated Gamma greater identify Lineage Mutation mutations N gene N501Y PCR Rapid Reactions reliability S gene Sanger sequencing SARS-CoV-2 SARS-CoV-2 virus selected SNP spike Spread statistical unique variant variants weight whole-genome sequencing [DOI] 10.3390/v14010123 PMC 바로가기 [Article Type] Article
Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia콜롬비아에서 전염병이 발생한 첫해 샘플에서 SARS-CoV-2 게놈의 돌연변이 프로필Article Published on 2022-01-012022-09-11 Journal: Infection, genetics and evolution : journal of mol [Category] SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus base pairs Beta CDC circulating clade collected concern coronavirus correlation COVID-19 D614G diagnostic diagnostic test disease severity E484K Effectiveness Evidence followed by Gamma genetic change genetic information Genetic variation genomic genomic variant GISAID Health Organization High-throughput nucleotide sequencing host immunity interest L18F lAbel Lineage Missense MOST Mutation next generation sequencing NGS non-structural proteins nucleotide ORF1ab gene pandemic predominant recent regions S gene SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 variants sequenced South America structural protein structural proteins Surveillance synonymous the SARS-CoV-2 genome the WHO Vaccines variants virus VoC WHO Whole genome sequencing World Health Organization [DOI] 10.1016/j.meegid.2021.105192 PMC 바로가기 [Article Type] Article