High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activityArticle Published on 2020-12-252023-07-06 Journal: The Journal of Biological Chemistry [Category] SARS, [키워드] angiotensin converting enzyme 2 carboxypeptidase enzymatic activity fluorescence resonance energy transfer (FRET) Pathogenesis renin angiotensin system SARS-CoV-2 spike protein Viral protein [DOI] 10.1074/jbc.RA120.015303 PMC 바로가기
Designed variants of ACE2-Fc that decouple anti-SARS-CoV-2 activities from unwanted cardiovascular effectsAnti-SARS-CoV-2 활동을 원치 않는 심혈관 영향으로부터 분리하는 ACE2-Fc 변형 설계Article Published on 2020-12-152022-09-11 Journal: International journal of biological macromolecules [Category] SARS, 변종, [키워드] ACE2 ACE2-Fc Alter angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Anti-SARS-CoV-2 Activity antibody antiviral therapies Antiviral treatment balance benefit binding candidate catalytic catalytic activity Cell culture COVID-19 Effect entry receptor enzymatic activity enzyme evaluated facilitate fusion protein immune clearance intrinsic Mutagenesis mutants performed pharmacological physiological Pseudotyped virus recombinant ACE2 remained SARS-CoV-2 Side effect substrate substrates suppress tested transduction Treatment variant Vasoactive virus [DOI] 10.1016/j.ijbiomac.2020.10.120 PMC 바로가기 [Article Type] Article
Candidate Binding Sites for Allosteric Inhibition of the SARS-CoV-2 Main Protease from the Analysis of Large-Scale Molecular Dynamics SimulationsLetter Published on 2020-12-112022-10-31 Journal: The journal of physical chemistry letters [Category] COVID-19, MERS, SARS, [키워드] Analysis analyzed approach binding site candidate catalytic dyad conformation Contact Data analysis Dynamics enzymatic activity free energy landscape in some inhibition less protease residue Simulation Site solvent the SARS-CoV-2 trajectory variables [DOI] 10.1021/acs.jpclett.0c03182 PMC 바로가기 [Article Type] Letter
Identification of Small Molecule Inhibitors of the Deubiquitinating Activity of the SARS-CoV-2 Papain-Like Protease: in silico Molecular Docking Studies and in vitro Enzymatic Activity AssayChemistry Published on 2020-12-082022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] activity Antiviral Antiviral effect approach binding binding site candidate canonical catalytic caused Characteristics cleave Compound compounds Consequences coronavirus COVID-19 Critical crystal structures deubiquitinase deubiquitinase inhibitors docking Effects enzymatic activity evaluate functional gallate high affinity Hypericin identification immune regulation in silico in vitro indicated inhibited inhibiting inhibitor inhibitors mechanism molecular docking molecule pandemic Papain-like protease polypeptides pp1a pp1ab protease Protease activity Protease inhibitor Proteases public health Replication SARS-CoV-2 Seven shown small molecule subsequent the SARS-CoV-2 the SARS-CoV-2 virus these compound trials [DOI] 10.3389/fchem.2020.623971 PMC 바로가기 [Article Type] Chemistry
The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2Genome Replication and Regulation of Viral Gene Expression Published on 2020-09-162022-10-30 Journal: Journal of Virology [Category] COVID-19, SARS, [키워드] activities activity acute respiratory syndrome affecting Alpha Amino acid antiviral drug Betacoronavirus betacoronaviruses boost can be used complex coronavirus CoV CoVs Critical decrease displaying domains enzymatic activity enzyme evaluated example Exoribonuclease Frequency Genetic genetics genome replication guanine-N7-methyltransferase In vitro assay MERS-CoV MHV Middle East MONITOR mouse hepatitis virus mRNA mutant mutants Mutation nidovirus nonstructural protein nsp14 Nsps observation occurred phenotype Phenotypes progeny promote Proofreading purified replicase replicate Replication replication fidelity replicative reported respiratory syndrome coronavirus reverse genetics RNA genome RNA synthesis SARS-CoV SARS-CoV-2 sequence identity single mutant subunit Support thought viral RNA synthesis viruses while [DOI] 10.1128/JVI.01246-20 PMC 바로가기 [Article Type] Genome Replication and Regulation of Viral Gene Expression
COVID-19: A Review on Diagnosis, Treatment, and ProphylaxisReview Published on 2020-07-212022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, SARS, [키워드] ACE2 addition Affect angiotensin Anti-inflammatory drugs antibody attenuate binding CD4+ T cell CD8+ clinical trial common cold contribute coronavirus 2 correlated country CoV COVID-19 infection develop diffusion drug Efficacy enzymatic activity Frequency functional healthcare identify immune immune system immunization in viral infect Infection involved measure Neutralizing antibodies outbreak outcome pandemic Patient Perspective Pneumonia positive prevention receptor Replication resources respiratory review SARS-CoV2 SARS-CoV2 infection severe respiratory syndrome severity single-stranded RNA virus Symptom the disease Tocilizumab Transcription Treatment turn Vaccine virus [DOI] 10.3390/ijms21145145 PMC 바로가기 [Article Type] Review
A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery박테리아 세포 신장 기계에 의한 펩티도글리칸 중합의 활성화에서 PBP2의 중심 역할Research Article Published on 2018-10-182022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Actin activate activated Activation activation pathway activities activity Analysis antibiotic Author Bacteria bacterial cell biogenesis Cell Cell elongation cell wall cell wall synthesis changes in complex complexes component conserved Control control cell crosslinking cytokinesis cytoskeletal filament cytoskeletal filaments determine disrupt enzymatic activity enzyme Enzymes Escherichia coli Evidence exhibited Genetic genetic evidence glycan glycan polymerase glycan polymerases hyperactivate identify in vitro in vivo inactivated morphogenic MreB Mutation osmotic lysis pathway PBP PBP2 PBPs penicillin penicillin-binding protein penicillin-binding proteins polymerase Prevent promote PROTECT provide remained required Rod system RodA stimulate the cell uncrosslinked variant [DOI] 10.1371/journal.pgen.1007726 PMC 바로가기 [Article Type] Research Article
In Situ Tagged nsp15 Reveals Interactions with Coronavirus Replication/Transcription Complex-Associated ProteinsResearch Article Published on 2017-01-312022-10-29 Journal: mBio [Category] Coronavirus, [키워드] association biochemical carried complexes conserved coronavirus Coronavirus infection Coronaviruses CoV CoV infection CoV protein CoV replication defined determine domain Endoplasmic reticulum enzymatic activity enzyme epitope function genomic RNA help independent Infection interact Interaction interactions involved knowledge membrane MHV mouse hepatitis virus nonstructural protein not affect Nsp12 Nsp15 Nsps P/S polymerase Protein replaced Replication replication complex replicative required RNA RTC Tagged Transcription Viral protein viral replication virus virus replication while [DOI] 10.1128/mBio.02320-16 PMC 바로가기 [Article Type] Research Article
The Anti-sigma Factor RsiV Is a Bacterial Receptor for Lysozyme: Co-crystal Structure Determination and Demonstration That Binding of Lysozyme to RsiV Is Required for σ V Activation항-시그마 인자 RsiV는 리소자임에 대한 박테리아 수용체입니다: 공결정 구조 결정 및 RsiV에 대한 라이소자임의 결합이 σ V 활성화에 필요하다는 입증Research Article Published on 2016-09-072022-09-17 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] absence acting activate activated Activation active site addition Bacteria Bacterial bind binding cascade Cell cell wall cleavage cleave complex crystal structure Defense demonstrated demonstration Determination enzymatic activity enzyme Evidence Factor function functions Gram-positive bacteria inhibit inhibitor initiated innate immune lysozyme many case mutant promoter protease Proteases Protein proteolytic receptor regulated required resistance mechanisms RIP RNA polymerase sensor signal Signal transduction specificity Structure suggested susceptible Target genes the cell These data thought Trigger triggering triggers X-ray [DOI] 10.1371/journal.pgen.1006287 PMC 바로가기 [Article Type] Research Article
Structural and functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virusArticle Published on 2010-04-142022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] activities Ala Amino acid amino acid substitution Analysis Atlantic salmon bovine Bovine coronavirus catalytic site Combination determinant different disease domain enzymatic activity enzyme expression feature functional had no Hemagglutinin-esterase Hemagglutinin-esterase (HE) infectious Infectious salmon anaemia virus (ISAV) Influenza introduced molecular orthomyxovirus Protein protein structure Proteins purification Quantitative Receptor binding Receptor-destroying enzyme (RDE) Recombinant secretory HE (recHE) red blood cells representing resolved responsible selected Ser Serine Structural analysis Surface glycoprotein variants virulence virulent virus [DOI] 10.1016/j.virusres.2010.03.020 PMC 바로가기 [Article Type] Article