Reactive T Cells in Convalescent COVID-19 Patients With Negative SARS-CoV-2 Antibody SerologySARS-CoV-2 항체 혈청 검사가 음성인 회복기 COVID-19 환자의 반응성 T 세포Immunology Published on 2021-07-122022-09-11 Journal: Frontiers in Immunology [Category] SARS, 치료기술, 치료법, [키워드] analyzed Antibody Response CD4+ T cell convalescent convalescent COVID-19 patient convalescent individuals convalescent patient Convalescent patients coronavirus Coronavirus disease 2019 (COVID-19) COVID-19 COVID-19 patients cytokine detectable disease course elicited Endemic flow cytometry Frequency HCoV HCoV-229E healthy control healthy controls human endemic coronavirus 229E (HCoV-229E) human endemic coronavirus OC43 (HCoV-OC43) IFN IL-2 Mild negative nucleocapsid peptide peptide pool persistence positive question raise reactive RT-PCR SARS-CoV-2 SARS-CoV-2 spike seronegative seropositive serum severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specific antibodies specific antibody Stimulation T cell T cell response T cells TNFα undetectable Viral viral control [DOI] 10.3389/fimmu.2021.687449 PMC 바로가기 [Article Type] Immunology
A bivalent protein targeting glycans and HR1 domain in spike protein potently inhibited infection of SARS-CoV-2 and other human coronavirusesResearch Published on 2021-07-082022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] Antiviral component conducted coronavirus COVID-19 Diseases E. coli effective EK1 ELISA evaluate exhibited expressing GL25E glycan GRFT HCoV-229E HCoV-NL63 HCoV-OC43 HIV HR1 domain human coronavirus in vitro in vivo Infection inhibit inhibited inhibiting inhibitory inhibitory activity intranasally mechanism of action MERS-CoV mice mutants mutated Newborn Ni-NTA peptide performed Prevent previous study Prophylactic protective effect Protein Proteins pseudovirus purified Result S protein S2 subunit SARS-CoV SARS-CoV-2 SARS-COV-2 infection selected shown significantly more Spike protein the S1 subunit therapeutic treat were expressed wild-type [DOI] 10.1186/s13578-021-00638-w PMC 바로가기 [Article Type] Research
Adaptation of the endemic coronaviruses HCoV-OC43 and HCoV-229E to the human hostResearch Article Published on 2021-06-242022-10-28 Journal: Virus Evolution [Category] COVID-19, [키워드] accessory proteins accompanied accumulated adaptation antigenic antigenic drift applied approach binding affinity both virus both viruses cellular receptor class coronavirus Coronaviruses driven by Endemic endemic coronavirus event Evolution evolutionary dynamics generate HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HCoV-OC43 spike Hemagglutinin-esterase higher affinity highly pathogenic human cell human host human populations Hypothesis I, II Immunity indicated Infection Like Limited genetic diversity Membrane proteins molecular dating molecular evolution much higher Positive selection Protein RBD RBDs Receptor binding Result SARS-CoV-2 evolution selected selective Spike protein strain suggested targets the RBD the receptor-binding domain the spike protein time interval trajectory undergo virus zoonotic zoonotic origins [DOI] 10.1093/ve/veab061 PMC 바로가기 [Article Type] Research Article
Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variantsResearch Article Published on 2021-06-182022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, SARS, [키워드] approach B.1.1.7 B.1.351 binding caused characterized circulating Combined CoVs Critical develop effective vaccine effort fusion inhibitor fusion protein global public health HCoV-229E HCoV-NL63 human CoVs impacted in viral Infection information inhibiting inhibitor inhibitory activity lipopeptide mechanism membrane fusion MERS-CoV Mutation pandemic of COVID-19 peptide peptides SARS-CoV SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variants second sequence South Africa Spike protein stability strain structural property Treatment United Kingdom variant [DOI] 10.1080/22221751.2021.1937329 PMC 바로가기 [Article Type] Research Article
Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detectionArticle Published on 2021-06-172022-10-29 Journal: Nature Communications [Category] COVID-19, SARS, [키워드] Computer science coronavirus detection diagnose diagnostics disease Final HCoV-229E help infections MERS-CoV Nanopore Nanopores Novel coronavirus Outbreaks platform point-of-care Rapid RNA extraction RT-PCR Saliva SARS-CoV SARS-CoV-2 sensitivity specificity specimen viral infection Viral RNA virus [DOI] 10.1038/s41467-021-24001-2 PMC 바로가기 [Article Type] Article
Prospective Assessment of SARS-CoV-2 Seroconversion (PASS) study: an observational cohort study of SARS-CoV-2 infection and vaccination in healthcare workersSARS-COV-2 SEROCONVERSION (PASS) 연구의 전향 적 평가 : 의료 근로자의 SARS-COV-2 감염 및 예방 접종에 대한 관찰 코호트 연구Study Protocol Published on 2021-06-092022-09-01 Journal: BMC Infectious Diseases [Category] MERS, SARS, 진단, [키워드] Affect Analysis Anti-spike antibody assessment Asymptomatic baseline clinical response Cohort Complete conducted coronavirus coronavirus disease Coronavirus disease 2019 CoV covariates COVID-19 COVID-19 disease COVID-19 disease course cross-reactivity cytokine production determine develop disease symptoms evaluate Frequency glycoprotein HCoV HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HCoVs HCWs healthcare healthcare worker HKU1 human coronavirus IgG antibodies IgM and IgG immune response immune responses Immune-response immunological individual Infection intensity Killer cell magnitude mononuclear cell natural natural killer cell NL63 number of infected Observational cohort study OC43 pandemic coronavirus participant pauci-symptomatic PBMCs peptide peptide pool performed Peripheral blood Peripheral blood mononuclear cells positive Prospective Prospective Study questionnaire receive respiratory Respiratory disease Respiratory illness SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 PCR SARS-CoV-2 PCR testing SARS-CoV-2 vaccination SARS-CoV-2 vaccine seroconvert serology analysis severe respiratory illness severity Spike proteins supplementary material Symptom T cell T cell activation T cell response T cell responses tested the SARS-CoV-2 the spike protein vaccination Vaccine viral respiratory with COVID-19 [DOI] 10.1186/s12879-021-06233-1 PMC 바로가기 [Article Type] Study Protocol
Developing a Paper-Based Antigen Assay to Differentiate between Coronaviruses and SARS-CoV-2 Spike Variants코로나바이러스와 SARS-CoV-2 스파이크 변이체를 구별하기 위한 종이 기반 항원 분석 개발Article Published on 2021-06-082022-09-11 Journal: Analytical Chemistry [Category] SARS, 변종, [키워드] Antigen binding build Concentration COVID-19 cross-reactivity Developing diagnostics Factors Final flexible fraction Fractions global pandemic HCoV HCoV-229E HCoV-OC43 HKU1 human saliva human serum immunoassay less lowest media pandemic PCR produced provide robust RT-PCR SARS-CoV-1 SARS-CoV-2 significant effect spike Spike protein Spike proteins test line time-consuming unique various coronaviruses viruses Wuhan Wuhan, China [DOI] 10.1021/acs.analchem.0c05438 PMC 바로가기 [Article Type] Article
Comparative Study of SARS-CoV-2, SARS-CoV-1, MERS-CoV, HCoV-229E and Influenza Host Gene Expression in Asthma: Importance of Sex, Disease Severity, and Epithelial Heterogeneity천식에서 SARS-COV-2, SARS-COV-1, MERS-COV, HCOV-229E 및 인플루엔자 숙주 유전자 발현의 비교 연구 : 성별, 질병 중증도 및 상피 이질성의 중요성Comparative Study Published on 2021-06-052022-09-10 Journal: Viruses [Category] MERS, SARS, 유전자 메커니즘, [키워드] ACE2 ACE2 receptor activated airway allergy ANPEP Asthma asthmatic both sexes cells changes in Characteristics characterized ciliated cell ciliated cells club Comparative coronavirus COVID-19 disease downregulation DPP4 epithelial epithelium expression Factor female GAL4 Gene Expression goblet HCoV HCoV-229E heterogeneity human coronavirus Importance increase in Inflammation Influenza influenza viral lack MERS MERS-CoV Mild moderate molecular occurred Patient protease Proteases receptor SARS SARS-CoV-1 SARS-CoV-2 severity Sex sexual dimorphism SLC6A19 ST3GAL4 ST6GAL1 susceptibility Th2 TMPRSS2 TMPRSS4 transcriptomic data upregulated Viral viral entry viral infection viral infections virus [DOI] 10.3390/v13061081 PMC 바로가기 [Article Type] Comparative Study
A severe case of human coronavirus 229E pneumonia in an elderly man with diabetes mellitus: a case reportCase Report Published on 2021-06-042022-10-28 Journal: BMC Infectious Diseases [Category] COVID-19, MERS, [키워드] acute respiratory distress adult respiratory distress syndrome Atrial fibrillation Care Case report clinical collected complement component coronavirus disease COVID-19 Critical described diabete discharged extensive lung damage HCoV-229E hospital human coronavirus human coronavirus 229E immunocompetent adults immunological immunology Infection Intensive intensive care pandemic pathogen Patient Pneumonia progression pulmonary inflammation radiological reported respiratory infection Severe case Support syndrome T cell treated Treatment weakness [DOI] 10.1186/s12879-021-06188-3 PMC 바로가기 [Article Type] Case Report
TMEM41B is a host factor required for the replication of diverse coronaviruses including SARS-CoV-2TMEM41B는 SARS-CoV-2를 비롯한 다양한 코로나바이러스의 복제에 필요한 숙주인자Research Article Published on 2021-05-272022-08-13 Journal: PLoS Pathogens [Category] SARS, 유전자 메커니즘, [키워드] added alphacoronavirus Antiviral antiviral therapeutics approach approaches benefit Betacoronavirus broad spectrum Characteristics characterized cholesterol Complete complex complexes conserved contribute coronavirus COVID-19 pandemic Critical curvature Defense difficulty Diseases Endemic facilitate Factor Factors functions Future HCoV-229E health systems Host host cell host protein host proteins human coronavirus inhibit Interaction lead lifecycle Lipid loss of function membrane mobilization mutants mutate pandemic pathway performed Prevent Protein Replication replication complexes required RNA SARS-CoV-2 Stage Structure Tmem41b Viral viral escape Viral protein Viral proteins viral replication virus viruses while [DOI] 10.1371/journal.ppat.1009599 PMC 바로가기 [Article Type] Research Article