Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification루프매개 등온증폭법을 이용한 신종 코로나바이러스 감염증(COVID-19)의 신속한 진단 평가Article Published on 2020-05-212022-09-25 Journal: Journal of Clinical Virology [Category] MERS, SARS, 진단, [키워드] acute respiratory syndrome amplification amplified can be used Care cDNA comparable condition coronavirus COVID-19 detection Detection limit Detection sensitivity Diagnosis diagnostic DNA amplification Effectiveness evaluated febrile influenza A isothermal isothermal amplification Japanese LAMP nasopharyngeal swab sample novel coronavirus disease Patient point-of-care protocol Quantitative reagent reverse transcription PCR RNA RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity specificity specimen Spread transcriptase utility [DOI] 10.1016/j.jcv.2020.104446 PMC 바로가기 [Article Type] Article
Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosisSARS-CoV-2 감염 진단을 위한 신속 IgM-IgG 복합항체 검사 개발 및 임상적용Research Article Published on 2020-04-132022-09-06 Journal: Journal of medical virology [Category] Communicable Disease, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus addition Antibody test Asymptomatic Asymptomatic carrier Asymptomatic carriers Blood blood sample Blood samples can be used carried carriers Chain Reaction China Clinical diagnosis Clinical efficacy Clinical studies clinical study clinics collected coronavirus coronavirus disease COVID‐19 COVID‐19 patient COVID‐19 detect Detection sensitivity development Diagnosis eight evaluated fingerstick blood hospitals identify IgG antibodies IgG antibody IgG test IgM Immunoglobulin immunoglobulin M indicated infected patients Infection lateral flow immunoassay limitations negative patients Novel coronavirus novel coronavirus disease nucleic acid nucleic acid real‐time polymerase chain reaction number of infected outbreak Patient patients PCR plasma point‐of‐care point‐of‐care testing polymerase chain polymerase chain reaction Prevent rapid IgM‐IgG combined test Rapid test real‐time PCR test reported respiratory SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 virus SARS‐CoV‐2 virus infection sensitivity serum serum and plasma severe acute respiratory syndrome Coronavirus specificity Spread stages symptomatic Treatment utility venous venous blood virus virus transmission were measured [DOI] 10.1002/jmv.25727 PMC 바로가기 [Article Type] Research Article
An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020Rapid Communication Published on 2020-04-092022-10-31 Journal: Eurosurveillance [Category] COVID-19, SARS, [키워드] caused COVID-19 Denmark Detection sensitivity diagnostics molecular molecular diagnostics pandemic reagent risk RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA subsequent World Health Organization [DOI] 10.2807/1560-7917.ES.2020.25.14.2000398 PMC 바로가기 [Article Type] Rapid Communication
A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human CoronavirusesArticle Published on 2016-11-232022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] Acute respiratory infection characteristic clinical clinical samples coronavirus demonstrated detection Detection sensitivity Diagnosis distribution fluorescent HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 hospitalized patient Human human coronaviruses IMPROVE Infection management melting Melting curve melting curve analysis melting temperature (Tm) multiplex quantitative RT-PCR (RT-qPCR) multiplex RT-qPCR primer Rapid respiratory virus RT-qPCR Sequencing Simultaneous SYBR Green I SYTO 9 temperature Treatment was used [DOI] 10.3390/ijms17111880 PMC 바로가기 [Article Type] Article
Evaluation of a novel chemiluminescent microplate enzyme immunoassay for hepatitis B surface antigen detectionArticle Published on 2016-02-012023-06-16 Journal: Journal of virological methods [Category] B형 간염, [키워드] Detection sensitivity Genotype hepatitis B surface antigen mutants Serotype. [DOI] 10.1016/j.jviromet.2015.11.013 PMC 바로가기
Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronavirusesArticle Published on 2012-10-302022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] active infections assay bovine colonies coronavirus detect detected Detection sensitivity diagnose diagnostic different fecal Hepatitis Infection infections isothermal amplification Laboratory Loop-mediated isothermal amplification MHV molecular mouse mouse hepatitis virus performed prevalent virus Rat coronavirus Rodent coronavirus RT-LAMP RT-PCR sensitivity Sensitivity and specificity specificity specimen strain virus [DOI] 10.1016/j.jviromet.2012.10.008 PMC 바로가기 [Article Type] Article