Designing of a bispecific antibody against SARS-CoV-2 spike glycoprotein targeting human entry receptors DPP4 and ACE2인간 진입 수용체 DPP4 및 ACE2를 표적으로 하는 SARS-CoV-2 스파이크 당단백질에 대한 이중특이성 항체 설계Article Published on 2022-04-012022-09-11 Journal: Human immunology [Category] COVID19(2023년), SARS, 바이오마커, 진단, [키워드] ACE2 Anti-viral antibody binding affinity Bispecific Antibody (BsAb) block China cleavage condition COVID-19 CrossMAb D614G disease DPP4 entry receptor epithelial cells Fc region global pandemic glycoprotein hACE2 hACE2 receptor heavy chain Host in-silico Infection KIH light chain linker lung alveolar lung alveolar epithelial cells modifications Molecular docking study mutated peptide performed Prevent receptor receptor engagement receptors regdanvimab Research SARS-CoV-2 SARS-CoV-2 spike glycoprotein spike spike glycoprotein Spike protein therapeutic target therapy Viral viral entry viral spike viral spike protein virus Wuhan [DOI] 10.1016/j.humimm.2022.01.004 PMC 바로가기 [Article Type] Article
Development of Receptor Binding Domain (RBD)-Conjugated Nanoparticle Vaccines with Broad Neutralization against SARS-CoV-2 Delta and Other VariantsSARS-CoV-2 Delta 및 기타 변이체에 대한 광범위한 중화를 갖는 수용체 결합 도메인(RBD)-접합 나노입자 백신의 개발Article Published on 2022-04-012022-09-11 Journal: Advanced Science [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] B.1.617.2 Beta Breakthrough infection breakthrough infections cross-protective Delta delta variant Delta variants develop development dominant hACE2 Incubation period induce Infection mice NAb NAbs nanoparticle nanoparticle vaccines. Neutralizing antibodies neutralizing antibody notable omicron Omicron variant Other PROTECT Receptor binding domain recipients rhesus macaque rhesus macaques robust SARS-CoV-2 SARS-CoV-2 delta SARS-CoV-2 variant SARS-CoV-2 variants significantly strain Strains Transmission trivalent Vaccine Vaccines variant Viral Viral load VoC [DOI] 10.1002/advs.202105378 PMC 바로가기 [Article Type] Article
Emerging of composition variations of SARS-CoV-2 spike protein and human ACE2 contribute to the level of infection: in silico approachesSARS-CoV-2 스파이크 단백질과 인간 ACE2의 조성 변화가 감염 수준에 기여함: in silico 접근법Article Published on 2022-04-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] ACE2 angiotensin bind binding affinity binds bioinformatics calculated Combination contribute COVID-19 detect determine docking domain eight FIVE Genetic variant global energy hACE2 human ACE2 human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 in silico in silico Approach in silico software Interaction investigated Laboratory moderate NCBI nsSNPs pandemic pathogenic PPI processed Protein Protein docking protein-protein interaction RBD RBD variant RBD variants Receptor-binding domain retrieved risk of infection SARS-CoV SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 spike protein sequence similarity significantly SNP SNPs Support the binding affinity V367F V483A variant variants Variation zone [DOI] 10.1080/07391102.2020.1841032 PMC 바로가기 [Article Type] Article
HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodiesHYBRiD: 포유류의 몸을 청소하기 위한 하이드로겔 강화 DISCOArticle Published on 2022-04-012022-09-11 Journal: Nature methods [Category] COVID19(2023년), MERS, SARS, 진단, 치료제, [키워드] antibody Chest compatibility component components demonstrated dimensions DISCO feature fluorescent hACE2 heterogeneous hybrid immunostaining K18-hACE2 transgenic mice macroscopic mammalian mice nervous system Newborn offer organ organic parvalbumin Protein reporter Research SARS-COV-2 infection solvent three dimensions translational utility Viral viral pathology Visualization [DOI] 10.1038/s41592-022-01427-0 PMC 바로가기 [Article Type] Article
Evaluating Antibody Mediated Protection against Alpha, Beta, and Delta SARS-CoV-2 Variants of Concern in K18-hACE2 Transgenic MiceK18-hACE2 형질전환 마우스에서 우려되는 알파, 베타 및 델타 SARS-CoV-2 변이체에 대한 항체 매개 보호 평가Article Published on 2022-03-232022-09-11 Journal: Journal of Virology [Category] MERS, SARS, 변종, 진단, 치료법, [키워드] Alpha Alpha VOC angiotensin angiotensin converting enzyme Beta Brain can be used concern convalescent convalescent plasma COVID-19 COVID-19 pandemic COVID-19 patient Critically ill decrease Delta Delta VOC disease dose Efficacy elevated enzyme evaluate Evaluating hACE2 hcp Human IgG titer immune evasion immunization in vivo model K18 K18-hACE2 K18-hACE2 transgenic mice lethality lung Mediated mice modeling modeling COVID-19 monoclonal antibody Mortality not decreased nucleocapsid nucleocapsid IgG pandemic passive immunity passive immunization polyclonal antibodies polyclonal antibody problem protection RBD Receptor binding domain response SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variants serum single dose Strains survival These data transgenic Transgenic mouse Treatment treatment group Vaccine variants variants of concern Viral Viral RNA while [DOI] 10.1128/jvi.02184-21 PMC 바로가기 [Article Type] Article
Computational Modeling of T Cell Hypersensitivity during Coronavirus Infections Leading to Autoimmunity and Lethality자가 면역 및 치사율로 이어지는 코로나바이러스 감염 중 T 세포 과민증의 전산 모델링Article Published on 2022-03-222022-09-11 Journal: Computational and Mathematical Methods in Medicine [Category] SARS, 변종, [키워드] Analysis angiotensin antiviral drug Autoimmune response autoimmune responses Autoimmunity binding CD147 COVID-19 COVID-19 treatment Critical Delta determinant determinants develop docking domain hACE2 human Angiotensin-converting enzyme Hyperactivation implication in silico indicated individuals infected with SARS-CoV-2 Interaction investigated involved isoform leading lethality memory T cell memory T cells MERS MERS-CoV modeling molecular molecular docking molecular dynamics not affect omicron Omicron variants outcome outcomes Patient performed polar interaction polar interactions Protein binding provide receptor receptors residue resulting SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein severe COVID-19 shown Spike protein suggested T cell T cells the SARS-CoV-2 transmembrane glycoprotein Treatment variants of SARS-CoV-2 viral spike protein [DOI] 10.1155/2022/9444502 PMC 바로가기 [Article Type] Article
Improved Binding Affinity of Omicron’s Spike Protein for the Human Angiotensin-Converting Enzyme 2 Receptor Is the Key behind Its Increased Virulence인간 안지오텐신 전환 효소 2 수용체에 대한 오미크론 스파이크 단백질의 향상된 결합 친화도는 독성 증가의 핵심입니다Article Published on 2022-03-212022-09-11 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Amino acid amino acid residue amino acids angiotensin Antiviral agents binding binding free energies binding free energy characterized complex contribute coronavirus country D38 domain electrostatic interaction enzyme exhibited free energy genomic region hACE2 hACE2 receptor Human human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 human angiotensin-converting enzyme 2 (hACE2) hydrogen hydrogen bonding Improved Increased information Interaction investigated mode molecular molecular dynamics Molecular dynamics simulation Molecular mechanics molecular mechanics-generalized Born surface area (MM-GBSA) mutated Mutation omicron Omicron variant Other pi-stacking RBD receptor Receptor-binding domain Receptor-binding domain (RBD) receptor-binding motif Receptor-binding motif (RBM) residue resulting salt bridges SARS-CoV-2 SARS-CoV-2 RBD Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) spike Spike protein surface area the RBD the spike protein Transmissibility tyrosine variant variant of SARS-CoV-2 virus wild type [DOI] 10.3390/ijms23063409 PMC 바로가기 [Article Type] Article
Short-Term Instantaneous Prophylaxis and Efficient Treatment Against SARS-CoV-2 in hACE2 Mice Conferred by an Intranasal Nanobody (Nb22)비강 나노바디(Nb22)에 의해 부여된 hACE2 마우스의 SARS-CoV-2에 대한 단기 순간 예방 및 효율적인 치료Article Published on 2022-03-172022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 변종, 진단, [키워드] addition Administered Against alpaca binding blocked Breakthrough infection breakthrough infections caused circulating Community Complete COVID-19 vaccine current Delta delta variant demonstrated doses effective Efficacy efficient exhibited exhibiting hACE2 highlight immunized Inhaled inoculation instantaneous instantaneous prophylaxis instantaneous protection intranasal long-lasting nanobodies nanobody Nb22 neutralization PrEP Prophylaxis RBD reported respiratory Respiratory system SARS-CoV-2 Seven single dose Spike protein stability STIP Structural analysis Structure the spike protein therapeutic unmet need variants [DOI] 10.3389/fimmu.2022.865401 PMC 바로가기 [Article Type] Article
An engineered nano-liposome-human ACE2 decoy neutralizes SARS-CoV-2 Spike protein-induced inflammation in both murine and human macrophages조작된 나노 리포솜 인간 ACE2 미끼는 쥐와 인간 대식세포 모두에서 SARS-CoV-2 스파이크 단백질 유도 염증을 중화합니다Article Published on 2022-03-062022-09-11 Journal: Theranostics [Category] SARS, 유전자 메커니즘, [키워드] abrogate abrogated ACE2 activating Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 binding biotinylated canonical CCL-2 chemokines competitive inhibitor complex coronavirus CXCL-10 cytokine D614G deficiency DEGs demonstrated differentially expressed gene Effect enzyme expressing expression fusion generate glycoprotein hACE2 human ACE2 human host cells human macrophage human macrophages IKKβ IKKβ/NF-κB signaling IL-1β IL-6 Immune cell immune cells in vitro in vivo Infection Inflammation Inflammatory response Inflammatory responses inhibit SARS-CoV-2 inhibitor internalization Intravenous injection IκB lentivirus Liposome Liposome-Human ACE2 Macrophage macrophages mechanism mice molecular murine myeloid-specific IKKβ knockout. neutralize NF-κB NF-κB activation NF-κB signaling Protein rationale receptor RNA sequencing SARS-CoV-2 SARS-CoV-2 spike glycoprotein severe acute respiratory syndrome Coronavirus Signaling spike spike glycoprotein Spike protein stimulated Therapeutic approach tissue inflammation TNFα Treatment was used wild type [DOI] 10.7150/thno.66831 PMC 바로가기 [Article Type] Article
Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 ExpressionK18-hACE2 마우스의 치명적인 신경 보급 및 SARS-CoV-2 향성은 hACE2 발현에 부분적으로만 의존합니다Article Published on 2022-03-052022-09-11 Journal: Viruses [Category] SARS, 치료기술, [키워드] ACE2 ACE2 protein ACE2-expressing cell ACE2-expressing cells Analysis angiotensin animal animal models AT1 axonal transport blood-brain barrier Brain bronchiolar Cell Cohort comparative pathology COVID-19 Critical cytokeratin cytokeratin 18 promoter days post-infection death Dependent detectable dose ENhance epidemiological data euthanasia evaluated Evidence expressing expression hACE2 Human human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 hypothermia immunohistochemistry In situ hybridization in vivo in vivo imaging independent independent of inoculated intranasal K18-hACE2 Lineage Mild-to-moderate neuroepithelium Neuron neurons occur olfactory olfactory bulb olfactory neuroepithelium overexpression pathologic PFUs Pneumocytes Pneumonia portal question resulting SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pathogenesis spatiotemporal subsequent transgenic mice translational translational animal model Transmission transmission electron microscopy Transport tropism vasculature viral pathogenesis Viremia Virological [DOI] 10.3390/v14030535 PMC 바로가기 [Article Type] Article