Contact residue contributions to interaction energies between SARS-CoV-1 spike proteins and human ACE2 receptorsArticle Published on 2021-01-132022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] amino acid residues Biophysics cellular receptors cryo-EM dominant hACE2 Health human ACE2 receptor human Angiotensin-converting enzyme human cells include Inclusion Interaction interactions molecular biophysics performed Protein Proteins receptor Receptor binding domain residue S-RBD SARS virus SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 virions significantly spike Spike protein structural biology van der Waal Virology virus X-ray crystallography [DOI] 10.1038/s41598-020-80942-6 PMC 바로가기 [Article Type] Article
Stapled ACE2 peptidomimetics designed to target the SARS‐CoV ‐2 spike protein do not prevent virus internalizationFull Paper Published on 2021-01-082022-10-28 Journal: Peptide Science (Hoboken, N.j.) [Category] COVID-19, [키워드] ACE2 antiviral activity assays binding caused cell entry coronavirus COVID‐19 enzyme human ACE2 receptor immunofluorescence inhibit Interaction motif neutralization Novel coronavirus peptide peptidomimetic PPI Prevent protein‐protein interaction pseudovirus RBD RBD binding required SARS‐CoV‐2 SARS‐CoV‐2 Spike protein stapled peptides virus were assessed [DOI] 10.1002/pep2.24217 PMC 바로가기 [Article Type] Full Paper
Cross-species recognition of SARS-CoV-2 to bat ACE2ACE2에 대한 SARS-CoV-2의 종간 인식530 Published on 2021-01-052022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] 변종, [키워드] Rhinolophus macrotis ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 attenuate bACE2 bat bat ACE2 bats benefit bind binding binding domain caused complex conserved coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Evidence expressing global health hACE2 Health host cell host cells human ACE2 human ACE2 receptor infect Infection Interaction less molecular basis mutant pandemic pseudotyped pseudotyped SARS-CoV-2 virus RBD RBD binding receptor Receptor binding domain residue residues respiratory SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 receptor SARS-CoV-2 spike protein receptor binding domain SARS-CoV-2 virus severe acute respiratory syndrome Coronavirus Significance Spike protein the SARS-CoV-2 Variation variations virus virus infection was determined Y41H [DOI] 10.1073/pnas.2020216118 PMC 바로가기 [Article Type] 530
Temperature dependence of the SARS-CoV-2 affinity to human ACE2 determines COVID-19 progression and clinical outcomeResearch Article Published on 2020-12-162022-10-31 Journal: Computational and Structural Biotechnology Journal [Category] COVID-19, [키워드] Abstract ACE2 affinity Analysis bind binding binding affinity body temperature Caco-2 cells cause Clinical data Clinical outcome Coronaviruses COVID-19 COVID-19 patients COVID-19 progression demonstrated determine early stage Efficiency enzyme II evidenced explained febrile Fever homolog human ACE2 human ACE2 receptor human cell indicated Infection Inflammatory response less Meta-analysis Molecular dynamics simulation patients penetrate protective factor Protein pseudovirus entry SARS-CoV SARS-CoV-2 SARS-CoV-2 virus Spike protein structural basis temperature Temperature dependence the binding affinity the SARS-CoV-2 Vero viral infection Viral protein viral spike protein [DOI] 10.1016/j.csbj.2020.12.005 PMC 바로가기 [Article Type] Research Article
Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding DomainsACE2가 있거나 없는 SARS-CoV-2 스파이크의 Cryo-EM 구조는 수용체 결합 도메인의 엔도솜 위치를 매개하는 pH 의존적 스위치를 나타냅니다Article Published on 2020-12-092022-09-12 Journal: Cell Host & Microbe [Category] MERS, SARS, 진단, [키워드] absence ACE2 ACE2 binding ACE2 receptor adopted Affect alteration binding antibody conformation COVID-19 Cryo-electron microscopy domain domains endosomal endosomal entry engage facilitate human ACE2 receptor immune evasion Interaction occur Pathways pH-dependent switch positioning RBD RBDs Receptor-binding domain Receptor-binding domain (RBD) required SARS-CoV-2 SARS-CoV-2 spike serological spike structural rearrangement Structure switch trimer type 1 fusion machine. virus entry [DOI] 10.1016/j.chom.2020.11.004 PMC 바로가기 [Article Type] Article
Structural basis for potent neutralization of SARS-CoV-2 and role of antibody affinity maturationArticle Published on 2020-10-272022-10-04 Journal: Nature Communications [Category] MERS, SARS, 변종, [키워드] antibody affinity Betacoronavirus bind binding binding affinity competing complex COVID-19 pandemic COVID19 determine effective epitope human ACE2 receptor induce infected with SARS-CoV-2 maturation mechanism monoclonal antibody motif Mutation neutralization Neutralizing neutralizing antibody overlap Patient Receptor binding domain reduction in responsible reveal SARS-CoV-2 the S1 subunit Treatment Vaccine viral infection virus X-ray X-ray crystallography [DOI] 10.1038/s41467-020-19231-9 PMC 바로가기 [Article Type] Article
Development of humanized tri-specific nanobodies with potent neutralization for SARS-CoV-2Article Published on 2020-10-202022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, SARS, [키워드] Analysis antibodies antibody Antibody therapy approach binding Combination coronavirus COVID-19 COVID-19 pandemic described develop development domain epitope Features fused generate Health human ACE2 receptor human IgG1 Fc humanized identify impacted Interaction lack monoclonal naïve nanobody neutralization protein stability resulting SARS-CoV-2 SARS-CoV-2 pseudovirus SARS-CoV-2 spike protein severity therapeutic therapy Treatment unmet need [DOI] 10.1038/s41598-020-74761-y PMC 바로가기 [Article Type] Article
A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19Article Published on 2020-10-152022-10-04 Journal: Nature Communications [Category] MERS, SARS, 신약개발, 임상, 진단, 치료법, 치료제, [키워드] A549 cell anti-infective drug anti-infective drugs Antiviral antivirals approved can be used Cell culture clinically Concentration Concordance coronavirus coronavirus disease COVID-19 evaluate expressing facilitate High-throughput screening human ACE2 receptor identify inactive Infection inhibitor neutralization neutralizing antibody patient sera plaque reduction neutralization platform PRNT replicate SARS-CoV-2 selective Serological testing severe respiratory syndrome Vaccine wild-type virus [DOI] 10.1038/s41467-020-19055-7 PMC 바로가기 [Article Type] Article
Genomic Diversity and Hotspot Mutations in 30,983 SARS-CoV-2 Genomes: Moving Toward a Universal Vaccine for the “Confined Virus”?Article Published on 2020-10-102022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Analysis analyzed binding Characteristics circulating collected Complete country COVID-19 COVID-19 pandemic distribution Divergence Diversity effective eight ENhance Evolution exonuclease Frequency Genetic genomes genomic diversity GISAID database greater Helicase HIV hotspot hotspot mutations human ACE2 receptor Influenza virus Mutation mutation type Non-synonymous Non-synonymous mutation NSP2 Nsp3 nucleocapsid protein ORF1ab ORF3a ORF8 Perspective polyprotein Prevalence RBD RdRP recurrent mutation SARS-CoV-2 SARS-CoV-2 genome spike Spike protein Spread stability Strains the disease the receptor-binding domain the SARS-CoV-2 the spike protein transmembrane domain understanding Universal Vaccine variant virus viruses Wuhan, China [DOI] 10.3390/pathogens9100829 PMC 바로가기 [Article Type] Article
Hot spot profiles of SARS-CoV-2 and human ACE2 receptor protein protein interaction obtained by density functional tight binding fragment molecular orbital methodArticle Published on 2020-10-082022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, SARS, [키워드] addition Amino acid Analysis analyzed Anti-viral antibodies binding complex complexes Computational biology and bioinformatics Consensus Coronaviruses develop FMO functional G354 global pandemic hACE2 hACE2 receptor HCoV-NL63 hot spot human ACE2 receptor human Angiotensin-converting enzyme information inhibit Interaction International K353 molecular PPI Prevalence profile Protein Protein analysis Protein–protein interaction public health emergency residue residues SARS-CoV-1 SARS-CoV-2 spike glycoprotein Spike proteins the SARS-CoV-2 the spike protein therapeutic Vaccine Viral proteins WHO β-coronavirus [DOI] 10.1038/s41598-020-73820-8 PMC 바로가기 [Article Type] Article