Isoelectric point determination by imaged CIEF of commercially available SARS-CoV-2 proteins and the hACE2 receptor상업적으로 이용 가능한 SARS-CoV-2 단백질 및 hACE2 수용체의 이미지화된 CIEF에 의한 등전점 측정Article Published on 2021-03-012022-09-11 Journal: Electrophoresis [Category] SARS, 치료기술, [키워드] a minor acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid ampholyte angiotensin calculated center CIEF condition conditions contribute coronavirus COVID-19 ExPASY fluorescence detection hACE2 hACE2 receptor human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 investigated isoelectric point isoforms minor portal Protein Proteins ProtParam tool RBD Receptor-binding domain Research respiratory S1 subunit S1/S2 S1/S2 subunit S1/S2 subunits SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins sequence severe acute respiratory syndrome Coronavirus the receptor-binding domain the S1 subunit theoretical pI [DOI] 10.1002/elps.202100015 PMC 바로가기 [Article Type] Article
Flexibility and mobility of SARS-CoV-2-related protein structuresSARS-CoV-2 관련 단백질 구조의 유연성 및 이동성Article Published on 2021-02-192022-08-31 Journal: Scientific Reports [Category] MERS, 신약개발, 치료제, [키워드] antiviral drug Biochemistry Biological physics Biophysics Complete Computational biology and bioinformatics database develop Docking studies docking study domain drugs example flexibility identify in silico in vitro in vivo information movement pandemic PDB Protein protein structures Proteins resolved rigidity S1 subunit SARS-CoV-2 drug SARS-CoV-2 spike SARS-CoV-2 spike protein Scientific community Structure structures targets the S1 subunit the SARS-CoV-2 Vaccine Viral proteins [DOI] 10.1038/s41598-021-82849-2 PMC 바로가기 [Article Type] Article
Cryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changesArticle Published on 2021-01-082022-10-29 Journal: Nature Communications [Category] Coronavirus, MERS, [키워드] activated alphacoronavirus Analysis antibody binding conformational change conformational state conformations coronavirus cryo-EM structure Cryoelectron microscopy glycoprotein HCoV-229E host cell host cell membranes Host receptors implication Interaction involved mechanism observé postfusion prefusion promote receptor Receptor binding reported reveal S trimer S-protein S1 subunit S1-NTD S2 subunit spike glycoprotein Spike protein Structure the S1 subunit therapeutic transmembrane glycoprotein trimeric type I Vaccine viral entry Viral membrane fusion Virus structures Virus-host interactions [DOI] 10.1038/s41467-020-20401-y PMC 바로가기 [Article Type] Article
COVID-19 variants that escape vaccine immunity: Global and Indian context-are more vaccines needed?백신 면역을 벗어나는 COVID-19 변종: 전 세계 및 인도 상황에서 더 많은 백신이 필요합니까?Review Published on 2021-01-012022-09-11 Journal: Journal of biosciences [Category] MERS, 변종, 진단, [키워드] ACE2 receptor Antigen bind Cell claimed complex convalescent plasma COVID-19 COVID-19 pandemic cryo-EM structure docked Efficacy Evolution exhibited followed by furin furin cleavage site Infection junction Mutation N terminal domain Neutralizing NTD occur proline RBM receptor binding motif residue responsible RNA virus S protein S1 domain S1 subunit S2 subunit SARS-CoV-2 selection pressure subunit the S1 subunit Vaccine vaccine candidate Vaccine development variant variants Viral viral envelope was used Wuhan [DOI] 10.1007/s12038-021-00226-7 PMC 바로가기 [Article Type] Review
Free SARS-CoV-2 Spike Protein S1 Particles May Play a Role in the Pathogenesis of COVID-19 Infection무료 SARS-CoV-2 스파이크 단백질 S1 입자는 COVID-19 감염의 병인에서 역할을 할 수 있습니다Hypothesis Published on 2020-12-302022-09-01 Journal: Biochemistry. Biokhimiia [Category] SARS, 변종, 진단, [키워드] accompanied ACE ACE2 angiotensin angiotensin (1-7) Angiotensin II Angiotensin-converting enzyme angiotensin-converting enzyme 2 association caused Cell cell surface circulation cleavage site COVID-19 COVID-19 disease COVID-19 infection COVID-19 pathogenesis D614G D614G mutation decrease disease downregulate downregulated Effect expression fraction Free furin furin protease higher infectivity Hypothesis Imbalance in vivo infected cell infected cells Infection Inflammation investigated lack lead less lung Lungs membrane-bound murine model mutant natural infection Particle Pathogenesis pathogenesis of COVID-19 Prevalence Protein pulmonary damage receptor-binding domains Recombinant spike protein Renin renin-angiotensin system reported role S protein S1 and S2 S1 subunit S1 subunit shedding SARS-CoV virus SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 strain separation severity shown spike Spike protein subunits the cell the receptor-binding domain the S protein the S1 subunit the SARS-CoV-2 thrombosis trimer unlikely Viral Viral load virion virions wild type [DOI] 10.1134/S0006297921030032 PMC 바로가기 [Article Type] Hypothesis
The S1 protein of SARS-CoV-2 crosses the blood–brain barrier in mice Research Published on 2020-12-162022-10-05 Journal: Nature Neuroscience [Category] 유전자 메커니즘, [키워드] acute respiratory syndrome Administered administration angiotensin-converting enzyme 2 ApoE bind Brain brain region cause Cell coronavirus coronavirus disease Effect Genotype indicated Inflammation injected intravenous intravenously involved kidney liver lung male mice murine not affect olfactory parenchymal S1 protein SARS-CoV-2 Sex Spike protein spleen the S1 subunit was reduced [DOI] 10.1038/s41593-020-00771-8 [Article Type] Research
Characterization of a Pan-Immunoglobulin Assay Quantifying Antibodies Directed against the Receptor Binding Domain of the SARS-CoV-2 S1-Subunit of the Spike Protein: A Population-Based StudyArticle Published on 2020-12-092022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] 95% CI 95% confidence interval acute respiratory syndrome antibody antibody level antibody reactivity area under the curve association Characteristics characterization Clinical characteristics clinically coronavirus coronavirus disease COVID-19 cross-reactivity detect diagnostic Diagnostic accuracy disease severity electrochemiluminescence immunoassay Epitopes evaluate Evidence Fever Follow-up help hospitalized patient identify IgA IgG IgM immunoassay individual interquartile range IQR Laboratory median offer Outpatient Patient predictive values proportion Quantitative RBD Receptor binding domain SARS-CoV-2 SARS-COV-2 infection sensitivity sera serology significantly increased smoker smoking specificity sustained symptom onset symptomatic tested the S1 subunit the SARS-CoV-2 the Spike the spike protein without symptom [DOI] 10.3390/jcm9123989 PMC 바로가기 [Article Type] Article
6-month SARS-CoV-2 antibody persistency in a Tyrolian COVID-19 cohort티롤리안 COVID-19 코호트에서 6개월 SARS-CoV-2 항체 지속성Original Article Published on 2020-12-092022-09-10 Journal: Wiener Klinische Wochenschrift [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus age All participant All participants Anti-viral anti-viral antibody antibody antibody response against Antibody testing Arthritis average binding antibody caused convalescent coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cohort cut-off cut-off point decrease Diagnosis disease disease onset dropped ELISA except for Follow-up healthy Hyposmia identify Immunity immunized individuals Infection information life Neutralizing Neutralizing antibodies neutralizing antibody participant Prospective Receptor binding domain respiratory Result rheumatoid arthritis S1 subunit SARS-CoV-2 antibody severe acute respiratory syndrome Coronavirus specificity Spike protein the S1 subunit the spike protein titers virus were used [DOI] 10.1007/s00508-020-01795-7 PMC 바로가기 [Article Type] Original Article
Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidateReport Published on 2020-11-272022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, [키워드] acute respiratory syndrome Analysis complexes coronavirus coronavirus disease COVID-19 ectodomain effort full-length full-length SARS-CoV-2 full-length spike protein host immune system immune response Interaction Microscopy Modification Neutralizing antibodies Novavax pandemic performed prefusion conformation provide responsible reveal SARS-CoV-2 SARS-CoV-2 spike glycoprotein Science Spike protein spike trimers stability Structure structures subunit the S1 subunit the vaccine Trimeric spike protein Vaccine vaccine candidate Vaccine development virus [DOI] 10.1126/science.abe1502 PMC 바로가기 [Article Type] Report
Cross-reactive neutralization of SARS-CoV-2 by serum antibodies from recovered SARS patients and immunized animals회복된 SARS 환자 및 면역된 동물의 혈청 항체에 의한 SARS-CoV-2의 교차 반응성 중화Article Published on 2020-11-062022-09-12 Journal: Science Advances [Category] SARS, 진단, [키워드] 2003 SARS acute respiratory syndrome analyzed Antisera caused collected convalescent serum coronavirus coronavirus disease Coronaviruses COVID-19 cross-neutralizing cross-reactive ectodomain Effect elicited full-length S immunized immunogen individual infections mice neutralization neutralize neutralized Novel coronavirus outbreak palm civet pandemic patient sera RBD Receptor binding domain recognize response S2 protein SARS patient SARS-CoV SARS-CoV infection SARS-CoV-2 SARS-CoV-2 S serum antibody the RBD the S1 subunit Vaccine [DOI] 10.1126/sciadv.abc9999 PMC 바로가기 [Article Type] Article