Phase separation by the SARS-CoV-2 nucleocapsid protein: Consensus and open questionsSARS-CoV-2 뉴클레오캡시드 단백질에 의한 상 분리: 합의 및 미해결 질문Review Published on 2022-02-042022-09-10 Journal: The Journal of Biological Chemistry [Category] COVID19(2023년), MERS, SARS, 치료법, [키워드] biomolecular condensate ERGIC, ER-Golgi intermediate compartment experiment experiments function genomic RNA gRNA, genomic RNA highlight IDR, intrinsically disordered region Infection Innate immunity intrinsically disordered region lab MD, molecular dynamics membraneless organelle modulation N-protein N, nucleocapsid nucleocapsid nucleocapsid protein observation organelle overlapping performed phase phase separation Protein PS, phase separation question required resource RNA RNP, ribonucleoprotein SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 pandemic stress granule the SARS-CoV-2 undergo Viral Viral protein Viral proteins viral replication virus viruses [DOI] 10.1016/j.jbc.2022.101677 PMC 바로가기 [Article Type] Review
High level SARS-CoV-2 nucleocapsid refolding using mild condition for inclusion bodies solubilization: Application of high pressure at pH 9.0고급 SARS-COV-2 NucleoCapsid 포함 물체 용량을위한 온화한 상태를 사용한 폴딩 : pH 9.0에서 고압의 적용Research Article Published on 2022-02-032022-09-10 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 진단, 치료법, [키워드] aggregate alkaline antibody Antibody binding Antigen application association Bacterial best binding condition conserved Coronaviruses Culture culture media described Diagnosis dialysis Disulfide E . coli effective electrostatic repulsion ELISA expressed for inclusion fragment of Host hydrophobic Inclusion inclusion bodies ionic interaction kDa knowledge media Mild motif non-specific Patient presenting promote promoted Protein purification purity SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 nucleocapsid sera solubilization tertiary structure Viral Viral protein western blotting yield [DOI] 10.1371/journal.pone.0262591 PMC 바로가기 [Article Type] Research Article
Development, Analytical, and Clinical Evaluation of Rapid Immunochromatographic Antigen Test for SARS-CoV-2 Variants DetectionArticle Published on 2022-02-022022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] Accuracy Ag-RDT Antigen Antigen rapid diagnostic test antigen test kit clinical clinically clinically evaluated COVID-19 detection develop development diagnostic effective evaluate evaluated independent infection control Kestrel Laboratory lateral flow limit of detection management nucleocapsid outbreak Outbreaks positive Protein Rapid Rapid diagnostic test SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 variant sensitivity specificity Spread Surveillance Test TM COVID-19 Ag Variant detection variety wild-type [DOI] 10.3390/diagnostics12020381 PMC 바로가기 [Article Type] Article
CTD of SARS-CoV-2 N protein is a cryptic domain for binding ATP and nucleic acid that interplay in modulating phase separationSARS-CoV-2 N 단백질의 CTD는 상분리를 조절하는 데 상호작용하는 ATP 및 핵산 결합을 위한 비밀 도메인입니다Article Published on 2022-02-012022-09-11 Journal: Protein Science : A Publication of the Protein Soc [Category] COVID19(2023년), SARS, 변종, 치료법, [키워드] affinity anti-SARS-CoV-2 ATP ATP: Nucleic acid bind binding binding domain cellular Complete Concentration Critical CTD domain drug target function generate gRNA host cell interface intrinsically life cycle liquid-liquid phase separation (LLPS) living cell modulating MOST N protein NMR NMR spectroscopy NTD nucleic acid nucleocapsid nucleocapsid (N) protein. offer pandemic probe Protein regions representing residue Result SARS-CoV-2 SARS-CoV-2 N protein SARS-CoV-2 nucleocapsid SARS-CoV-2 variants structures target targets the SARS-CoV-2 unique variant viral life cycle virus [DOI] 10.1002/pro.4221 PMC 바로가기 [Article Type] Article
Responses to a Neutralizing Monoclonal Antibody for Hospitalized Patients With COVID-19 According to Baseline Antibody and Antigen Levels : A Randomized Controlled Trial기준 항체 및 항원 수준에 따른 COVID-19 입원 환자의 중화 단일클론항체에 대한 반응: 무작위 대조 시험Randomized Controlled Trial Published on 2022-02-012022-09-11 Journal: Annals of internal medicine [Category] COVID19(2023년), MERS, SARS, 바이오마커, 임상, 진단, 치료기술, 치료법, 치료제, [키워드] allergy Analysis analyzed Anti-spike antibodies antibody Antigen bamlanivimab baseline benefit clinical trial collected Combination Controlled COVID-19 death defined detectable discharge elevated end-organ evaluate evidenced favor futility Government greater hazard hazard ratios hospitalized patient hospitalized patients Hypothesis immune IMPROVE independent Infectious diseases Interaction Level limited sample size median median time monoclonal antibody mounted Multicenter trial NAb NAbs nasal neutralizing antibody not differ operation Organ failure outcome participant Patient patients with COVID-19 Placebo placebo group placebo-controlled placebo-controlled trial plasma Randomized receiving Remdesivir required response SARS-CoV-2 nucleocapsid Serious Adverse Events serious infections serostatus small sample size specimen speed subgroup subgroup analysis sustained the median therapy Trial Viral Viral RNA with COVID-19 [DOI] 10.7326/M21-3507 PMC 바로가기 [Article Type] Randomized Controlled Trial
Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment장애 SARS-CoV-2 감염성 질병 평가를 위한 나노다이아몬드 조립된 금 맞물린 전극 상의 압타센싱 뉴클레오캡시드 단백질Article Published on 2022-02-012022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] active state analyses Analysis antibody aptamer Biomarker biosensor carbon Characteristics coefficient composed Corona Corona virus COVID-19 cubic demonstrated Detection limit diamond early diagnosis element elements enzyme evaluated evidenced facilitate functional gold group human serum indicate Infectious disease Interaction intrinsic linear nucleocapsid protein oxygen pandemic platform point-of-care Protein R 2 respiratory respiratory virus SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid SARS-CoV-2 pandemic silane stability strong interaction virus [DOI] 10.1016/j.bios.2021.113735 PMC 바로가기 [Article Type] Article
Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonanceArticle Published on 2022-01-202022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] coronavirus COVID-19 pandemic Detection sensitivity Gold nanoparticle highest Imaging and sensing limit of detection N protein Optical materials and structures Protein resulting SARS-CoV-2 N protein SARS-CoV-2 nucleocapsid significantly SPR the SARS-CoV-2 [DOI] 10.1038/s41598-022-05036-x PMC 바로가기 [Article Type] Article
Prevalence of antibodies against SARS-CoV-2 among pregnant women in Norway during the period December 2019 through December 20202019년 12월부터 2020년 12월까지 노르웨이 임산부의 SARS-CoV-2에 대한 항체 유병률Short Paper Published on 2022-01-132022-09-11 Journal: Epidemiology and Infection [Category] 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adjusted analysed antenatal care antibodies antibody Antigen Compliance coronavirus detect DiaSorin Elecsy Elecsys Epidemiology estimating explained first trimester hospital IgG immunoassay incidence modelling Norway Occurrence positive test result pregnant pregnant women Prevalence prevalence of disease preventive measure preventive measures remained respiratory Roche Diagnostics S1/S2 S2 protein SARS SARS CoV SARS CoV-2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid SARS-CoV-2 virus sera serological seropositive serum severe acute respiratory syndrome Coronavirus study period syphilis test results the epidemic the SARS-CoV-2 virus women [DOI] 10.1017/S0950268822000073 PMC 바로가기 [Article Type] Short Paper
GP73 is a glucogenic hormone contributing to SARS-CoV-2-induced hyperglycemia Research Published on 2022-01-062022-10-05 Journal: Nature metabolism [Category] 치료기술, [키워드] abnormality acute respiratory syndrome Alter antibody blockade blood glucose circulating complications contribute contributing to coronavirus cultured cell elevated fasting blood Glucose hyperglycemia in vitro infected with SARS-CoV-2 Infection inhibit liver Lower lung metabolism mice molecular mechanism mouse-adapted SARS-CoV-2 multiple tissue overexpression patients with COVID-19 plasma Protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid secretion signaling pathway Spike protein stimulate stimulated [DOI] 10.1038/s42255-021-00508-2 [Article Type] Research
SARS-CoV-2 Nucleocapsid Plasma Antigen for Diagnosis and Monitoring of COVID-19코로나19 진단 및 모니터링을 위한 SARS-CoV-2 뉴클레오캡시드 혈장 항원Articles Published on 2022-01-042022-09-10 Journal: Clinical Chemistry [Category] SARS, 진단, 치료기술, 치료법, 치료제, [키워드] 95% CI acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition adjusted age Antigen antigenemia association Blood clinical sensitivity clinical specificity collected comparable Concentration coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cycle threshold decrease described detection determine diabetes Diagnosis diagnostic disease severity electrochemiluminescence immunoassay evaluate exhibited facilitate Frequency Hospitalization hypertension ICU ICU admission individuals individuals with COVID-19 Inpatients intensive care intensive care unit interquartile range Kruskal–Wallis log mixed-effects modeling monitoring multivariable logistic regression NAAT non-ICU nucleic acid nucleic acid amplification nucleocapsid antigen Odds ratio outpatients Patient patient triage patients plasma plasma sample plasma samples prognostic Rate respiratory Result SARS-CoV-2 SARS-CoV-2 nucleocapsid severe acute respiratory syndrome Coronavirus severity Sex upper respiratory [DOI] 10.1093/clinchem/hvab216 PMC 바로가기 [Article Type] Articles