PCR in Forensic Science: A Critical ReviewReview Published on 2024-03-292024-09-05 Journal: Genes [Category] update2024, [키워드] annealing denaturation DNA amplification polymerase chain reaction STR amplification [DOI] 10.3390/genes15040438 PMC 바로가기 [Article Type] Review
Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPsArticle Published on 2022-10-042022-11-15 Journal: Analytical Chemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] amplification annealing Antisense oligonucleotide AuNP denaturation detect Detection limit DNA Gold nanoparticle gold standard guanine intensity labeling Modification N-gene New nucleocapsid polymerase chain Rapid Result RNA RNA gene RNA viruses RT-qPCR SARS-CoV-2 simplicity suggested threshold throat swab [DOI] 10.1021/acs.analchem.2c03544 PMC 바로가기
Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO 2-based photocatalyst실내 감광성 TiO 2 기반 광촉매에 의한 다양한 변종 SARS-CoV-2 비활성화Article Published on 2022-04-142022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Antiviral Chain Reaction copper copper oxide coronavirus COVID-19 damaged delta variant denaturation disease effective ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay examined fluorescent fragmentation gel electrophoresis illumination inactivated inactivation infectious risk investigated mechanism mutant strain Night pandemic coronavirus photocatalyst polyacrylamide polymerase chain polymerase chain reaction PROTECT Quantitative quantitative polymerase chain reaction reduce reverse transcription RNA RNAs RT-qPCR SARS-CoV-2 SARS-CoV-2 virus SDS-PAGE severe acute respiratory syndrome Coronavirus sodium Spike protein Spike proteins Spread the SARS-CoV-2 virus titanium titanium dioxide variant virus [DOI] 10.1038/s41598-022-09402-7 PMC 바로가기 [Article Type] Article
Photothermal inactivation of universal viral particles by localized surface plasmon resonance mediated heating filter membrane국소화 된 표면 플라즈몬 공명 매개 가열 필터 멤브레인에 의한 보편적 바이러스 입자의 광열 불 활성화Article Published on 2022-02-022022-09-01 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] 50% tissue culture infective dose cells Chain Reaction coated components Crystal violet Culture deformation denaturation dose effective gold H1Npdm09 HFM inactivation infectious sample infectious samples infective Infective dose Influenza virus irradiation Mardin membrane membrane protein Membrane proteins Nanoparticles Nanophotonics and plasmonics Natural hazards polymerase chain polymerase chain reaction provide qRT-PCR Quality control Quantitative reduce reduction reduction in reverse transcription room temperature SARS-CoV-2 staining surface plasmon surface plasmon resonance surface temperature TCID temperature tissue tissue culture Viral viral gene viral genes viral infectivity viral particle virus titer was reduced [DOI] 10.1038/s41598-022-05738-2 PMC 바로가기 [Article Type] Article
A Multi-Disulfide Receptor-Binding Domain (RBD) of the SARS-CoV-2 Spike Protein Expressed in E. coli Using a SEP-Tag Produces Antisera Interacting with the Mammalian Cell Expressed Spike (S1) ProteinArticle Published on 2022-02-012022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, [키워드] Escherichia coli expression accelerate ACE2 addition Analysis Antisera arginine residue binding activity C-terminus demonstrated denaturation disulfide bond E. coli elicited ELISA Epitopes Escherichia coli expressed expression Final FIVE fusion tag Host IgG immune response immunogenicity Inclusion mice nine overcome peptide Produce RBD receptor resulting scattering significantly solubility soluble fraction spike Spike protein the receptor-binding domain the SARS-CoV-2 Wuhan-Hu-1 [DOI] 10.3390/ijms23031703 PMC 바로가기 [Article Type] Article
SARS-CoV-2 N Protein Induces Acute Lung Injury in Mice via NF-ĸB ActivationSARS-CoV-2 N 단백질은 NF-ĸB 활성화를 통해 쥐의 급성 폐 손상을 유발합니다Immunology Published on 2021-12-072022-09-11 Journal: Frontiers in Immunology [Category] SARS, 치료법, [키워드] acute lung injury acute respiratory syndrome addition administration antibody blocked body fluid body fluids Bone marrow C57BL/6 cell count Contamination coronavirus disease Coronavirus disease 2019 COVID-19 Cytokines denaturation elevated expression in vitro induce Infection inhibitor Injury intratracheal administration lipopolysaccharide LPS lung Lung injury Macrophage macrophage polarization macrophages mice N-protein neutrophil neutrophil infiltration NF- kappa B nuclear nucleocapsid (N) protein nucleocapsid protein p65 pathway Phosphorylation Polymyxin B produced Proinflammatory cytokine proinflammatory cytokines promoted Protein PYRROLIDINE pyrrolidine dithiocarbamate recombinant reported respiratory Result SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 nucleocapsid therapeutic target therapeutic targets toxic Treatment triggered [DOI] 10.3389/fimmu.2021.791753 PMC 바로가기 [Article Type] Immunology
Denaturation of the SARS-CoV-2 spike protein under non-thermal microwave radiationArticle Published on 2021-12-032022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] body temperature cause Chemical biology comparable COVID-19 denaturation Effect eliminating facilitate host cell intensity Microbiology Protein Radiation SARS-CoV-2 Spike protein temperature the S1 subunit the SARS-CoV-2 therapeutic viral entry virus was performed widespread [DOI] 10.1038/s41598-021-02753-7 PMC 바로가기 [Article Type] Article
Sensitivity of SARS-CoV-2 towards Alcohols: Potential for Alcohol-Related Toxicity in HumansReview Published on 2021-12-032022-10-29 Journal: International Review of Cell and Molecular Biology [Category] COVID-19, [키워드] absence acute respiratory syndrome addition adverse effect alcohol alcohol-based hand sanitizers atopic dermatitis contact dermatitis contagious coronavirus COVID-19 death denaturation dermal/pulmonary toxicity drug effective Ethanol evaluate food gel Human humans isopropanol Masks measure mechanism of action medications organism pandemic positive Potential Prevent Protein reported responsible SARS-CoV-2 sensitivity single-stranded RNA virus social distancing Spread Toxicity Transmission transmission of SARS-CoV-2 virus World Health Organization [DOI] 10.3390/life11121334 PMC 바로가기 [Article Type] Review
The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous ExpressionSARS-COV-2 스파이크 수용체 결합 도메인 이종 발현의 견과류 및 볼트Article Published on 2021-12-022022-09-01 Journal: Biomolecules [Category] SARS, 유전자 메커니즘, 진단, [키워드] addition caused Circular coronavirus COVID-19 denaturation diagnostic domain effective ELISA Escherichia coli evaluated experiment filtration flow cytometry assay flow cytometry assays gel gel filtration HEK-293 cells heterologous expression indicated Infectious disease Neutralizing antibodies neutralizing antibody Nut Outbreaks pandemic produced progressed Protein protein production RBD Receptor-binding domain Recombinant SARS-CoV-2 RBD SARS-CoV-2 spike Spike protein surface Spike the SARS-CoV-2 Vaccine [DOI] 10.3390/biom11121812 PMC 바로가기 [Article Type] Article
An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2Microbiology Published on 2021-11-042022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Bio-rad chain clinical sensitivity clinical specificity Cohort COVID-19 denaturation detection extension Nasopharyngeal swab nasopharyngeal swab sample one-step RT-qPCR assay PCR cycle reaction reduce reduced RT-qPCR SARS-CoV-2 SARS-CoV-2 detection significantly ultrafast [DOI] 10.3389/fmicb.2021.749783 PMC 바로가기 [Article Type] Microbiology