In-depth blood proteome profiling analysis revealed distinct functional characteristics of plasma proteins between severe and non-severe COVID-19 patients심층 혈액 프로테옴 프로파일링 분석은 중증 코로나19 환자와 그렇지 않은 COVID-19 환자 사이에서 혈장 단백질의 뚜렷한 기능적 특성을 밝혀냈습니다.Article Published on 2020-12-292022-09-10 Journal: Scientific Reports [Category] MERS, SARS, 바이오마커, [키워드] Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis Biomarker Blood Candidates Characteristics Clinical outcome complement Complement activation coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients Critical case critical cases develop differentially expressed disease severity downstream eight Factor functional highlighting host response IMPROVE Infectious diseases interleukin interleukin-6 mortality rate Necrosis neutrophil neutrophil activation Patient patients worldwide performed plasma plasma protein Platelet potential therapeutic target potential therapeutic targets predict profiling analysis Prognosis prognostic biomarker Prognostic markers proinflammatory Protein Proteins proteome proteomic Proteomic analysis proteomics quantified reported respiratory SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severity of COVID-19 Support T cell Tumor tumor necrosis tumor necrosis factor upstream [DOI] 10.1038/s41598-020-80120-8 PMC 바로가기 [Article Type] Article
A distinct innate immune signature marks progression from mild to severe COVID-19뚜렷한 타고난 면역 신호는 경증에서 중증 COVID-19로의 진행을 나타냅니다.Article Published on 2020-12-262022-09-11 Journal: Cell Reports Medicine [Category] MERS, SARS, 유전자 메커니즘, 진단, [키워드] appearance CCL3 CCL4 CD16 CD169 coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients Cytometry emergency granulopoiesis healthy individuals identify IFN-γ immune responses immune signature Inflammation inflammatory phenotype Inflammatory response Inflammatory responses innate immune innate immune response innate response mass cytometry mechanism Mild mild COVID-19 patient mild COVID-19 patients mild or severe Covid-19 MIP1α monocyte Monocytes observé Patient progression proteomics sampling SARS-CoV-2 severe COVID-19 severe disease severe patients sialoadhesin sialoadhesin. signature Stage stages sustained symptom onset targeted intervention targeted interventions trajectory [DOI] 10.1016/j.xcrm.2020.100166 PMC 바로가기 [Article Type] Article
The SARS-CoV-2 RNA–protein interactome in infected human cellsArticle Published on 2020-12-212022-10-04 Journal: Nature Microbiology [Category] MERS, SARS, 치료제, [키워드] ATP1A1 cellular cellular pathway changes in CNBP complex Contact demonstrated evaluated Factor function Genetic Host host cell human cell human cell lines human cells human protein IMPROVE infected cell Infection innate immune response Interaction LARP1 PPIA Protein proteome proteomics purification reduced restrict RNA RNA metabolism SARS-CoV-2 SARS-CoV-2 infections SARS-CoV-2 replication SARS-CoV-2 RNA SARS-CoV-2 viral RNA the SARS-CoV-2 viral infection viral replication Viral RNA Virology [DOI] 10.1038/s41564-020-00846-z PMC 바로가기 [Article Type] Article
SARS-Cov-2 Interactome with Human Ghost Proteome: A Neglected World Encompassing a Wealth of Biological DataCommunication Published on 2020-12-192022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] addition Affinity purification alternative ORF alternative protein anti-viral response Biological cellular co-immunoprecipitation co-IP COVID-19 outbreak database DMPK Drug repurposing eukaryotic Evidence function functional Ghost ghost protein host cell Human identify initial innate immune response Interaction interactions interactome interactomics involved metabolism missing mRNA ncRNAs Neglected non-coding RNA OpenProt ORF Perspective pharmacokinetic physiological predicted Protein proteome proteomics responsible SARS-CoV-2 target the SARS-CoV-2 the SARS-CoV-2 virus Therapeutic strategies therapeutic targets Therapies thought translated translation Viral protein Viral proteins [DOI] 10.3390/microorganisms8122036 PMC 바로가기 [Article Type] Communication
Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2Report Published on 2020-12-182022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome affinity Anti-spike antibody Bacteria cells complex Concentration coronavirus COVID-19 pandemic group half-maximal inhibitory concentration identify immunization inactive indicated inhibit intravenously mammalian cell mechanisms microbe multivalent nanobodies nanobody neutralization neutralization potency neutralize Neutralizing nonoverlapping epitope potent Prevent produced proteomics RBD Receptor binding domain SARS-CoV-2 Science screened selected Spike protein stability synthetic nanobody the RBD the spike protein therapeutic viral infection yeast [DOI] 10.1126/science.abe4747 PMC 바로가기 [Article Type] Report
Optimized workflow for single-cell transcriptomics on infectious diseases including COVID-19Protocol Published on 2020-12-162023-06-17 Journal: STAR protocols [Category] MERS, SARS, [키워드] antibody bioinformatics clinical protocol Flow Cytometry/Mass Cytometry Gene Expression immunology proteomics RNA-Seq Sequence analysis Sequencing [DOI] 10.1016/j.xpro.2020.100233 PMC 바로가기 [Article Type] Protocol
CSF Levels of Elongation Factor Tu Is Associated With Increased Mortality in Malawian Adults With Streptococcus pneumoniae Meningitis스트렙토코쿠스 폐렴구균 수막염이 있는 말라위 성인의 생존율 증가와 관련된 엘롱게이션 팩터 Tu의 CSF 수치Original Research Published on 2020-12-112024-07-30 Journal: Frontiers in Cellular and Infection Microbiology [Category] 폐렴구균 감염증, [키워드] cerebrospinal fluid Elongation factor Tu (EF-Tu) HIV—human immunodeficiency virus Meningitis Mortality proteomics Streptococcus pneumoniae [DOI] 10.3389/fcimb.2020.603623 PMC 바로가기 [Article Type] Original Research
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19Multi-Omics는 경증과 중등도의 COVID-19 사이의 급격한 질병 상태 변화를 해결합니다Clinical Trial Published on 2020-12-102022-09-11 Journal: Cell [Category] SARS, 유전자 메커니즘, 임상, [키워드] accompanied amplify Analysis Blood blood clotting blood draw capture changes CITE-seq clinical measurements collected COVID-19 COVID-19 patient Diagnosis disease severity effective elevated feature identify immune Immune cell immune cells immune response Infection inflammatory signaling metabolic processes metabolite metabolites metabolomics Metrics Mild moderate disease multi Multi-omics phenotype plasma proteomics representing SARS-CoV-2 Secretome Sharp shift single-cell single-cell RNA-seq single-cell secretome. single-cell TCR-seq therapeutic intervention [DOI] 10.1016/j.cell.2020.10.037 PMC 바로가기 [Article Type] Clinical Trial
Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohortsArticle Published on 2020-12-032022-10-04 Journal: Nature Communications [Category] SARS, 바이오마커, 변종, [키워드] acute respiratory syndrome Analysis analyzed approach automated Cohort coronavirus COVID-19 pandemic detect develop Establishing mass spectrometry nasopharyngeal oropharyngeal swabs outbreak pandemic disease peptide peptide-based per day populations positive Proteomic analysis proteomics public health system real-time RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleoprotein SARS-CoV-2 testing specificity specimen stability swab sample temperature viral infection Viral proteins virus detection [DOI] 10.1038/s41467-020-19925-0 PMC 바로가기 [Article Type] Article
Data, Reagents, Assays and Merits of Proteomics for SARS-CoV-2 Research and TestingResearch Published on 2020-11-252022-10-30 Journal: Molecular & Cellular Proteomics : MCP [Category] COVID-19, SARS, [키워드] A549 ACE2 African green monkey Analysis approach BRCA1 Breast cancer Caco-2 Calu-3 can be used cause cell line change changed Chlorocebus clinical proteomics Cohort COVID-19 COVID-19 pandemic COVID-19 research diagnostic diagnostics evaluate Evidence expression expression data facilitate Factor feasibility genomes globe Innate immunity involved kDa label-free quantification manuscript mass spectrometry monitoring parallel reaction monitoring peer-reviewed peptides positive profile protease inhibitors Protein protein expression Proteins proteome proteomic proteomics Region representing Research SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 proteins SARS-CoV-2 research Spread SRP9 stable-isotope labeling supernatant synthetic synthetic peptide targeted mass spectrometry Testing TMPRSS2 transcriptional regulators Vero Cell Vero E6 Vero E6 cell viral entry viral infection virus widespread [DOI] 10.1074/mcp.RA120.002164 PMC 바로가기 [Article Type] Research