In silico comparison of SARS-CoV-2 spike protein-ACE2 binding affinities across species and implications for virus originSARS-COV-2 스파이크 단백질 -ACE2 결합 친화력의 SILICO 비교에서 종에 대한 바이러스 기원에 대한 영향Article Published on 2021-06-242022-09-10 Journal: Scientific Reports [Category] MERS, 유전자 메커니즘, [키워드] ACE2 affinity analyses angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 approach binding binding affinity can be used caused Chemistry Computational biology and bioinformatics Computational chemistry correlation COVID-19 pandemic Diseases docking Drug discovery enzyme exhibited explain ferret generate hACE2 help helping highest highest binding affinity highlight human ACE2 humans hydrogen Hydrogen bond hydrogen bonds implication in silico Infection information mechanism molecular dynamics Molecular dynamics simulation much lower novel virus Novel viruses pangolin power predict predicted Protein question raised respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 isolate SARS-CoV-2 spike sequence sequence homology sequence similarity shown Small molecules spike Spike protein structural biology structural homology susceptibility tested transfer Transmission vectors virus viruses [DOI] 10.1038/s41598-021-92388-5 PMC 바로가기 [Article Type] Article
A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patientsCOVID-19 환자에서 항-스파이크 항체의 혈청 학적 검출을위한 유세포 분석법 분석Protocol Published on 2021-06-192022-09-01 Journal: STAR protocols [Category] MERS, SARS, 진단, 치료기술, [키워드] Abstract anti-spike antibody antibody capture Cell Cell Biology cell surface Cloning complement Complete coronavirus coronavirus 2019 disease COVID-19 COVID-19 patient Cytometry detect Early detection eGFP Express Flow flow cytometry Flow Cytometry/Mass Cytometry full-length SARS-CoV-2 full-length SARS-CoV-2 spike protein Health Sciences immunology infected individual infected individuals insertion limit management Molecular biology patients with COVID-19 PCR-based assays Plasmid protocol public health S protein SARS-CoV-2 spike SARS-CoV-2 spike protein serological Serological assay serological assays specific antibodies specific antibody Spike protein the cell the S protein transduced transfection transfer Transmission Viral viral particle Viral particles [DOI] 10.1016/j.xpro.2021.100671 PMC 바로가기 [Article Type] Protocol
Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variantsResearch Article Published on 2021-06-182022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, SARS, [키워드] approach B.1.1.7 B.1.351 binding caused characterized circulating Combined CoVs Critical develop effective vaccine effort fusion inhibitor fusion protein global public health HCoV-229E HCoV-NL63 human CoVs impacted in viral Infection information inhibiting inhibitor inhibitory activity lipopeptide mechanism membrane fusion MERS-CoV Mutation pandemic of COVID-19 peptide peptides SARS-CoV SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variants second sequence South Africa Spike protein stability strain structural property Treatment United Kingdom variant [DOI] 10.1080/22221751.2021.1937329 PMC 바로가기 [Article Type] Research Article
Immunological imprinting of the antibody response in COVID-19 patientsCOVID-19 환자에서 항체 반응의 면역학적 각인Article Published on 2021-06-182022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 진단, 치료기술, 치료법, [키워드] 229E acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antibody antibody memory Antibody Response Antimicrobial responses Betacoronavirus boost conserved coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus infection Coronavirus infections COVID-19 COVID-19 pandemic COVID-19 patient Evidence evidence of HKU1 Hospitalized Human human coronavirus human coronaviruses humoral immune response Humoral immunity IgG and IgM immune response immunological Immunological memory infect Infection modulate nucleocapsid protein OC43 other coronavirus other coronaviruses pandemic Patient pre-existing immunity prevalence of COVID-19 Region Research respiratory response SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike seasonal coronavirus seasonal coronaviruses severe acute respiratory syndrome Coronavirus specific antibodies specific antibody Spike protein susceptible the antibody response variable regions viral infection [DOI] 10.1038/s41467-021-23977-1 PMC 바로가기 [Article Type] Article
Human IgG and IgA responses to COVID-19 mRNA vaccinesCOVID-19 mRNA 백신에 대한 인간 IgG 및 IgA 반응Research Article Published on 2021-06-162022-08-13 Journal: PLoS ONE [Category] Biochemistry, MERS, SARS, 진단, [키워드] Antigen average COVID-19 COVID-19 mRNA Vaccination COVID-19 mRNA vaccine COVID-19 mRNA vaccines elevated elicited highlight Human IgA IgA response IgG IgG level induce Infection information initial mRNA vaccination mRNA vaccine mRNA vaccines natural immunity peak levels persistence post vaccination remained responses SARS-CoV-2 spike SARS-CoV-2 spike antigen serum Serum level serum levels spike spike antigen-specific IgA subjects Vaccine vaccine dose Viral viral neutralization [DOI] 10.1371/journal.pone.0249499 PMC 바로가기 [Article Type] Research Article
Bioactive Polyphenolic Compounds Showing Strong Antiviral Activities against Severe Acute Respiratory Syndrome Coronavirus 2Article Published on 2021-06-152022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] activity Antiviral antiviral activity carried clinical study Compound compounds coronavirus 2 COVID-19 Curcumin determine early stage Effectiveness Evidence FDA-approved drug hesperidin Hydroxychloroquine in silico in vitro infections inhibited inhibitors mechanism of action molecular docking Molecular docking study MPro pharmacological protease quercetin receptors respiratory SARS-CoV-2 SARS-CoV-2 Mpro SARS-CoV-2 spike significantly Strong tested therapeutic targeting these compound VERO E6 cells viral infection virucidal virus replication [DOI] 10.3390/pathogens10060758 PMC 바로가기 [Article Type] Article
Potential transmission chains of variant B.1.1.7 and co-mutations of SARS-CoV-2Article Published on 2021-06-152022-10-04 Journal: Cell Discovery [Category] 바이오마커, 변종, [키워드] addition alternative hypothesis Analysis analyzed antibody B.1.1.7 bioinformatics Canidae demonstrated dominant Effect Evolution example Felidae Genomic analysis Host human populations Immune escape MONITOR Mutation Pathogenesis pathogenicity Potential provide raised reporter SARS-CoV-2 SARS-CoV-2 mutants SARS-CoV-2 mutations SARS-CoV-2 spike significantly suggested Surveillance the SARS-CoV-2 therapy Transmission United Kingdom variant viral genome viral replication whole-genome whole-genome sequences [DOI] 10.1038/s41421-021-00282-1 PMC 바로가기 [Article Type] Article
SARS-CoV-2 RBD trimer protein adjuvanted with Alum-3M-052 protects from SARS-CoV-2 infection and immune pathology in the lungAlum-3M-052가 보강된 SARS-CoV-2 RBD 삼량체 단백질은 폐의 SARS-CoV-2 감염 및 면역 병리로부터 보호합니다Article Published on 2021-06-112022-09-12 Journal: Nature Communications [Category] MERS, SARS, 진단, [키워드] alum Antibody Response Antibody titer Antigen cause changes in disease dose efficient ENhance function immune induce Infection inhibit innate cell Live virus long-lasting Longevity lung Lung pathology mice monomer Neutralizing antibodies Neutralizing antibody response Neutralizing antibody titer non-human primate non-human primates pathology potency PROTECT protective immunity Protein Protein vaccines provide RBD Receptor binding domain Receptor-binding domain rhesus macaques SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD trimer SARS-CoV-2 spike Spike protein the antibody response the RBD the receptor-binding domain the spike protein TLR7 TLR7/8 trimer trimeric vaccination Vaccine viral infection [DOI] 10.1038/s41467-021-23942-y PMC 바로가기 [Article Type] Article
Antibody Responses to SARS-CoV-2 Following an Outbreak Among Marine Recruits With Asymptomatic or Mild Infection무증상 또는 경증 감염이 있는 해병 모집단의 발병 후 SARS-CoV-2에 대한 항체 반응Immunology Published on 2021-06-092022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] Adults age antibodies Asymptomatic asymptomatic infection Betacoronavirus circulating Cohort comparable COVID-19 cross-reactivity disease domain Endemic enrolled focus healthy Humoral response humoral responses IgA antibodies IgA antibody IgG IgG antibodies IgG antibody Infection investigated Longitudinal studies longitudinal study Mild Mild disease mild SARS-CoV-2 infection mild symptoms Multiplex assay neutralization activity Neutralizing activity no decrease nucleocapsid outbreak participant polyclonal Population positive positive correlation Protein RBD recruit remained reported respiratory Respiratory illness response S protein S proteins SARS-CoV-2 SARS-CoV-2 IgG SARS-COV-2 infection SARS-CoV-2 RBD SARS-CoV-2 spike sera serological response significantly spring tested young adults zoonotic [DOI] 10.3389/fimmu.2021.681586 PMC 바로가기 [Article Type] Immunology
Neutralization potency of monoclonal antibodies recognizing dominant and subdominant epitopes on SARS-CoV-2 Spike is impacted by the B.1.1.7 variantSARS-CoV-2 스파이크에서 우성 및 하위 우성 에피토프를 인식하는 단일클론 항체의 중화 효능은 B.1.1.7 변이체의 영향을 받습니다.Article Published on 2021-06-082022-09-10 Journal: Immunity [Category] MERS, SARS, 변종, 진단, [키워드] Abstract Affect antibodies antibody antigenic antigenic drift antigenicity arising Asymptomatic B.1.1.7 B.1.1.7 variant characterized circulating SARS-CoV-2 variant circulating SARS-CoV-2 variants COVID-19 disease COVID-19 disease severity disease severity dominant Donor epitope Epitopes glycan host cell host cells Immune escape impacted individual infected individuals Infectious virus less monoclonal antibodies monoclonal antibody MOST Mutation N-terminal domain NAb NAbs neutralization neutralization activity Neutralizing activity Neutralizing antibodies neutralizing antibody neutralizing epitope neutralizing epitopes neutralizing monoclonal antibodies neutralizing monoclonal antibody NTD overlapping plasma potent RBD receptor ACE2 Receptor binding domain recognizing retained S2 subunit SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variants SARS-CoV-2-infected individuals specific antibodies spike The B.1.1.7 the RBD the SARS-CoV-2 variant variant of concern variants Viral viral entry [DOI] 10.1016/j.immuni.2021.03.023 PMC 바로가기 [Article Type] Article