mammalian cell – Page 5 – Communicable Diseases Research

Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2

Report

Published on 2020-12-182022-10-28
Journal: Science (New York, N.y.) [Category] COVID-19, MERS, SARS,

Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

게놈 RNA 요소가 SARS-CoV-2 뉴클레오캡시드의 상 분리를 유도합니다

Article

Published on 2020-12-172022-09-11
Journal: Molecular Cell [Category] SARS, 신약개발, 치료법, 치료제,

[키워드] aggregation Antiviral compound Antiviral compounds binding site body temperature buffer characterized concentrations condensation condition coronavirus crosslink drive effective element feature Genome genomic genomic region human body liquid location mammalian cell mammalian cells N-protein nucleocapsid nucleocapsid protein phase physiological promote reduced Region regulate RNA RNA sequence room temperature SARS-CoV-2 SARS-CoV-2, Condensation, phase separation, packaging, RNP-MaP, RNA structure, nucleocapsid, coronavirus. separation single-stranded Small molecules strength temperature the SARS-CoV-2 valency Viral Viral RNA
[DOI] 10.1016/j.molcel.2020.11.041 PMC 바로가기 [Article Type] Article

Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Article

Published on 2020-12-112022-10-29
Journal: Scientific Reports [Category] COVID-19, SARS,

Design of a highly thermotolerant, immunogenic SARS-CoV-2 spike fragment

내열성이 높은 면역원성 SARS-CoV-2 스파이크 단편의 설계

Research Article

Published on 2020-11-232022-09-12
Journal: The Journal of Biological Chemistry [Category] SARS, 진단,

[키워드] ACE2 antibodies AUC, area under the curve clinic clinical testing COVID-19 CPE, cytopathic effect Culture DMEM, Dulbecco's Modified Dulbecco's Medium ectodomain elicited Endpoint exposure to expressed extreme feature geometric mean geometric mean titer glycosylation guinea pig HRP, horseradish peroxidase IAEC, Institutional Animal Ethics committee IMAC, immobilized metal affinity chromatography immunogenic ingredient mammalian cell MF59 microbial monomeric mRNA Neutralizing antibodies neutralizing antibody NTD, N-terminal domain PBS, phosphate buffered saline PEI, polyethylenimine Pichia Protein subunit vaccines purified RBD RBD protein RBD, receptor-binding domain RBM, receptor binding motif Receptor-binding domain replicative SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 vaccine SEC, size-exclusion chromatography solution Spike protein subunit Subunit vaccine temperature the RBD the receptor-binding domain thermostable thermotolerance Vaccine viral spike protein Viral vector viral vectors virus
[DOI] 10.1074/jbc.RA120.016284 PMC 바로가기 [Article Type] Research Article

Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein

Article

Published on 2020-11-052022-10-29
Journal: Scientific Reports [Category] COVID-19, SARS,

[키워드] 293T biosafety convalescent patient Efficiency expressing full-length G glycoprotein glycoprotein hACE2 highly pathogenic IgG levels lentiviral lentivirus mammalian cell mammalian cells Microbiology much higher neutralized Particle Patient Preclinical research produced provide pseudotype pseudotyped Pseudotyped virus recovered COVID-19 patient retroviral SARS-CoV-2 SARS-CoV-2 neutralizing antibody SARS-CoV-2 receptor SARS-CoV-2 spike glycoprotein SEROLOGICAL STUDY serum specific IgM specific neutralizing antibody spike tested the SARS-CoV-2 transduced vesicular stomatitis virus Virology virus VSV-G while
[DOI] 10.1038/s41598-020-76135-w PMC 바로가기 [Article Type] Article

The Emergency Response Capacity of Plant-Based Biopharmaceutical Manufacturing-What It Is and What It Could Be

Plant Science

Published on 2020-10-202022-10-31
Journal: Frontiers in Plant Science [Category] COVID-19,

SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates

Research Article

Published on 2020-09-242022-10-31
Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS,

[키워드] acute respiratory syndrome aluminium adjuvant Analysis antigenicity appear candidate convalescent coronavirus coronavirus disease COVID-19 COVID-19 patient dose ectodomain elicit ELISA enzyme-linked immunosorbent evaluated expressed expression glycosylation highly pathogenic homotrimer immunization immunogenicity Inclusion induce insect cell insect cell expression system lowest mammalian cell mice Microscopy monomer neutralization non-human primate offer pandemic prefusion produced Protein RBD reactivity Receptor binding domain recombinant COVID-19 vaccine reduced regimen S protein S-2P S-protein S1 subunit SARS-CoV-2 SARS-CoV-2 spike sera spike subjects suggested the mean therapeutic titre Transmission trimer Vaccine wild-type
[DOI] 10.1080/22221751.2020.1821583 PMC 바로가기 [Article Type] Research Article

SARS‐CoV‐2 replicating in nonprimate mammalian cells probably have critical advantages for COVID‐19 vaccines due to anti‐Gal antibodies: A minireview and proposals

Review

Published on 2020-08-022022-10-04
Journal: Journal of medical virology [Category] Fulltext, MERS, 진단,

Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion

Article

Published on 2020-07-282022-10-28
Journal: Viruses [Category] COVID-19,

[키워드] alphacoronavirus alphacoronavirus nsp1 Analysis Basis Betacoronavirus Biological biological function Cell cell cycle cell cycle genes Cellular immune response characterized composed conserved creating crystal structure experiment full-length Host host cell host protein host proteins IFN IFN-I immune Infectious disease inhibit inhibiting inhibitory effect interfere interferon mammalian cell mechanism PEDV Phosphorylation proliferation Protein regulate regulated Regulatory Renilla luciferase RNA sequence RNA sequencing selected Seven significantly structure-function structures subtype TGEV treated Viral virulence virus western blotting β-CoV β-CoVs
[DOI] 10.3390/v12080812 PMC 바로가기 [Article Type] Article

Monoclonal antibodies for the S2 subunit of spike of SARS-CoV-1 cross-react with the newly-emerged SARS-CoV-2

Research

Published on 2020-07-162022-10-31
Journal: Eurosurveillance [Category] COVID-19, SARS,

[키워드] Ability ACE2 acute respiratory syndrome aligned angiotensin-converting enzyme 2 antibody binding cause Cell common-cold conserved Coronavirus disease 2019 Coronaviruses COVID-19 cross-react cross-reactive cross-reactive antibodies cross-reactivity demonstrated detect diagnostic domain ELISA expressed expressing full length host cell receptor human infections immunogenic interact mAb mAbs mammalian cell MERS Middle East monoclonal antibody murine Novel coronavirus outbreak Part performed Protein raised recombinant S protein responsible Result S protein S2 subunit sandwich ELISA SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 infected cell SARS-CoV-2 research SARS-CoV-2 S protein SARS-CoV-2-infected cell sequence spike glycoprotein Spike protein syndrome tested the S protein while
[DOI] 10.2807/1560-7917.ES.2020.25.28.2000291 PMC 바로가기 [Article Type] Research