Specific detection of memory T-cells in COVID-19 patients using standardized whole-blood Interferon gammarelease assay표준화된 전혈 인터페론 감마 방출 분석을 사용하여 COVID-19 환자에서 기억 T 세포의 특정 검출Article Published on 2021-12-012022-09-11 Journal: European Journal of Immunology [Category] SARS, 치료기술, [키워드] complementary COVID-19 patient immune immune memory interferon interferon gamma interferon gamma release assay Interferon-gamma memory MONITOR peptide peptide pools protective immunity SARS-CoV-2 SARS-CoV-2 immunity Serological assay serological assays Specific Stimulation T-cell T-cell Response T-cells T-cells response [DOI] 10.1002/eji.202149296 PMC 바로가기 [Article Type] Article
Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion인간 SARS-CoV-2 혈청전환을 검출하기 위한 뉴클레오캡시드 단백질에 기초한 간접 ELISA의 항원 생산 및 개발Article Published on 2021-12-012022-09-11 Journal: Brazilian journal of microbiology : [publication o [Category] MERS, 진단, 치료법, [키워드] Accuracy controls COVID-19 COVID-19 case COVID-19 cases described detect determine develop ELISA ELISA kit enzyme-linked immunosorbent enzyme-linked immunosorbent assay Escherichia coli exposure to expressed head Head-to-head comparison helping homogeneity human SARS-CoV-2 humoral Humoral response identify immunization Immunological test mass production nucleocapsid protein Protein purified qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 nucleocapsid sensitivity Sensitivity and specificity sera Seroconversion serological assays the SARS-CoV-2 Vaccines was used [DOI] 10.1007/s42770-021-00556-6 PMC 바로가기 [Article Type] Article
Long term persistence of coronavirus SARS-CoV-2 infection코로나바이러스 SARS-CoV-2 감염의 장기간 지속Case Reports Published on 2021-11-242022-09-11 Journal: Einstein [Category] MERS, SARS, 치료기술, [키워드] antibody Brazil Chain Reaction Coronavírus coronavirus SARS-CoV-2 COVID-19 COVID-19 pandemic COVID-19o described diagnosed with COVID-19 Final follow-up period hospital Infecções Coronavírus Infecções por coronavírus infecção Infection long term Marco molecular Pandemia Patient pelo persistence polymerase chain polymerase chain reaction reverse transcriptase SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus serological assays test results the patient the SARS-CoV-2 virus transcriptase Viral viral clearance Viral load [DOI] 10.31744/einstein_journal/2021RC6369 PMC 바로가기 [Article Type] Case Reports
Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects천연 SARS-COV-2 감염 후 중화 항체 반응의 역학 및 상업적 혈청 학적 시험과의 상관 관계.백신 접종 된 대상과의 재평가 및 간접 비교Comparative Study Published on 2021-11-222022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] Accuracy administration antibody binding Binding assays blood sample Blood samples BNT162b2 calculated Cohort COVID-19 COVID-19 patients cut-off cut-offs detect Dynamics epitope experiment first dose Follow-up gold gold standard healthcare worker Healthcare workers IgA IgG IgM IMPROVE majority NAb NAbs natural Neutralising Antibodies neutralization neutralization test neutralizing antibody neutralizing assays neutralizing capacity of BNT162b2 participant Patient plaque reduction neutralization Plaque reduction neutralization test positive PRNT pseudovirus pseudoviruses response RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections secure serologic Serological assay serological assays subjects surrogate virus neutralization test sVNT symptoms onset target cells the SARS-CoV-2 total antibodies total antibody two-dose regimen virus virus neutralization test was performed [DOI] 10.3390/v13112329 PMC 바로가기 [Article Type] Comparative Study
A Highly Sensitive and Specific SARS-CoV-2 Spike- and Nucleoprotein-Based Fluorescent Multiplex Immunoassay (FMIA) to Measure IgG, IgA, and IgM Class AntibodiesResearch Article Published on 2021-11-172022-10-30 Journal: Microbiology Spectrum [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome analyses antibody antibody concentration assays Biosafety Level 3 BSL-3 calibrated calibration class collected comparability Concentration Concordance coronavirus coronavirus disease correlated strongly correlation COVID-19 enzyme fluorescent gold standard identify IgA IgG IgM Immunity immunoassay IMPROVE Infection International measure microneutralization Microneutralization test MNT moderate multiplex NAb titer NAb titers NAbs neutralization test Neutralizing antibodies neutralizing antibody neutralizing antibody titers nucleoprotein pandemic persistence population immunity quantification Receptor-binding domain SARS-CoV-2 SARS-CoV-2 antibody sensitive sensitivity Serological assay serological assays Specific specificity spike spike glycoprotein Standard Standardization Surveillance Symptom the WHO vaccination validation WHO WHO International Standard World Health Organization [DOI] 10.1128/Spectrum.01131-21 PMC 바로가기 [Article Type] Research Article
A rapid quantitative on-site coronavirus disease 19 serological test신속 정량 현장 코로나바이러스 감염증 19 혈청학적 검사Article Published on 2021-11-012022-09-11 Journal: Biosensors & bioelectronics [Category] MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 assist clinical sample clinical samples convalescence coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patients daily monitoring Developed function IgM and IgG IgM/IgG immune reaction immune response immune system immunoassay IQR limit of detection median Microfluidics pandemic parameter Patient patients PCR negativity PCR positive POCT promote Quantitative Regulation respiratory respond SARS CoV SARS CoV-2 SARS-CoV-2 sensitivity Seroconversion Serological assay serological assays serological test severe acute respiratory syndrome Coronavirus simple specificity the mean titre turn unique Volume [DOI] 10.1016/j.bios.2021.113406 PMC 바로가기 [Article Type] Article
Combination of a SARS-CoV-2 IgG Assay and RT-PCR for Improved COVID-19 Diagnosis개선된 COVID-19 진단을 위한 SARS-CoV-2 IgG 분석 및 RT-PCR의 조합Article Published on 2021-11-012022-09-12 Journal: Annals of laboratory medicine [Category] MERS, SARS, 진단, [키워드] Abbott Laboratories acute respiratory syndrome ARCHITECT® caused Combination compensating coronavirus Coronavirus disease 2019 COVID-19 COVID-19 case COVID-19 diagnosis decrease diagnosed diagnostic diagnostics evaluate evaluated exceeded groups hospital IgG immunoassay IMPROVE Improved Japanese Laboratory Lake limitation positive positive result ranged Red Retrospective study reverse transcription Reverse transcription (RT)-PCR RT-PCR SARS-CoV-2 SARS-CoV-2 IgG SARS-CoV-2 viral load sensitivity serological assays Society specificity symptom onset Tokyo Transcription USA were used [DOI] 10.3343/alm.2021.41.6.568 PMC 바로가기 [Article Type] Article
An Assessment of Serological Assays for SARS-CoV-2 as Surrogates for Authentic Virus Neutralization진정한 바이러스 중화를 위한 대리인으로서 SARS-CoV-2에 대한 혈청학적 분석 평가Research Article Published on 2021-10-272022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus alkaline phosphatase assessment authentic SARS-CoV-2 virus best biosafety level Biosafety Level 3 Blood BSL3 caused characterized Concordance conditions coronavirus correlation COVID-19 pandemic Critical deaths disadvantage Disadvantages ELISAs embryonic enzyme-linked immunosorbent assay enzyme-linked immunosorbent assays fluorescent followed by future infection global pandemic gold green fluorescent protein identify immunoassays increasingly Infection level 3 luciferase neutralization Neutralization assay neutralization assays Neutralizing antibodies neutralizing antibody Neutralizing antibody response neutralizing antibody responses Neutralizing assay outbreak performed phosphatase presence of antibody Protective protective antibodies Protective antibody protective immunity Protein pseudotyped Pseudotyped virus Research respiratory resulting SARS-CoV-2 SARS-CoV-2 virus secreted Serological assay serological assays severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome virus stomatitis surrogate Surrogates vaccination Vaccine vesicular stomatitis vesicular stomatitis virus virus Virus neutralization VSV while [DOI] 10.1128/Spectrum.01059-21 PMC 바로가기 [Article Type] Research Article
Validation of Commercial SARS-CoV-2 Immunoassays in a Nigerian Population나이지리아 인구에서 상용 SARS-CoV-2 면역분석의 검증Research Article Published on 2021-10-062022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료법, [키워드] Abbott Algorithm anti-SARS-CoV-2 Architect assay Bio-rad caution Characteristics China confirmation country COVID-19 COVID-19 pandemic Disease prevalence effort ELISAs Endemic enzyme-linked immunosorbent assay enzyme-linked immunosorbent assays estimate Euroimmun Europe evaluated exposure to FIVE hepatitis B highlight HIV positive IgG immunoassay Immunoglobulin Immunoglobulin G IMPROVE independent lead limit Microparticle much lower negative sample Nigeria nucleocapsid protein Omega pathogen Pathogens PCR PCR-positive patient plasma Population populations positive public health reported SARS-CoV-2 SARS-CoV-2 IgG sensitivities sensitivity Sensitivity and specificity serological Serological assay serological assays serology Seroprevalence Serosurvey Serosurveys Specificities specificity specimen Spread Total validation widespread [DOI] 10.1128/Spectrum.00680-21 PMC 바로가기 [Article Type] Research Article
Sero-prevalence of anti-SARS-CoV-2 antibodies among healthcare workers: A multicenter study from Egypt의료 종사자들 사이에서 항 SARS-CoV-2 항체의 혈청 유병률: 이집트의 다기관 연구Multicenter Study Published on 2021-10-012022-09-11 Journal: Journal of infection and public health [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] age anti-SARS-CoV-2 antibodies anti-SARS-CoV-2 antibody antibody Antigen assays Asymptomatic carried conducted confirmation Contact contact with COVID-19 COVID-19 cases COVID-19 diagnosis COVID19 cross determine disease ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay female Frequency HCW HCWs healthcare healthcare worker Healthcare workers high risk highest hospital IgG IgM and IgG investigated Low-middle income multicenter nucleic acid nucleic acid testing Nurse Nurses PCR physician positive proportion reported resource risk for disease SARS-CoV-2 SARS-CoV-2 virus Screening serologic serologic assays Serological assay serological assays seropositive Seropositivity Seroprevalence specific antibodies specific antibody statistically significant difference the Spike the timing university hospital with COVID-19 [DOI] 10.1016/j.jiph.2021.09.011 PMC 바로가기 [Article Type] Multicenter Study