Severity, Pathogenicity and Transmissibility of Delta and Lambda Variants of SARS-CoV-2, Toxicity of Spike Protein and Possibilities for Future Prevention of COVID-19Review Published on 2021-10-182022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] ACE2 ACE2 receptor amino acid substitution antibody changes in claimed COVID-19 Delta delta variant Effective vaccines Epitopes furin furin protease Future future therapeutic high stability hotspot immune response Lambda Lambda variant less Mutation N protein NAbs neutralizing antibody NTD pathogenicity prevention Protein receptor S protein S proteins SARS-CoV-2 SARS-CoV2 severity spike spike protein toxicity the N protein the S protein Toxicity Treatment TRIM21 vaccinated individuals Vaccine development variant variants virus infection while World Health Organization Wuhan strain [DOI] 10.3390/microorganisms9102167 PMC 바로가기 [Article Type] Review
SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNASARS-COV-2 Nucleocapsid 단백질은 바이러스 게놈 RNA와 응축수를 형성한다Research Article Published on 2021-10-112022-09-10 Journal: PLoS Biology [Category] MERS, SARS, 유전자 메커니즘, 치료법, [키워드] acute respiratory syndrome coronavirus Alter asymmetric Base pairing cause coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 Cross-linking Deletion disrupt form genomic RNA global health Health host cells identify in vitro infected cells Infection inhibit Interaction interactions mammalian cells mass spectrometry mechanism membrane N protein N proteins nucleocapsid pandemic proliferation Protein respiratory RNA RNA genome SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 nucleocapsid severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 shape small molecule Small molecules substrate the N protein the SARS-CoV-2 the SARS-CoV-2 genome Viral viral genome viral genomic RNA viral particle Viral RNA virion while [DOI] 10.1371/journal.pbio.3001425 PMC 바로가기 [Article Type] Research Article
PABPC4 Broadly Inhibits Coronavirus Replication by Degrading Nucleocapsid Protein through Selective AutophagyResearch Article Published on 2021-10-062022-10-30 Journal: Microbiology Spectrum [Category] COVID-19, [키워드] activate addition alphacoronavirus Antiviral antiviral response Antiviral strategies autolysosome autophagy coronavirus Coronaviruses CoV CoV replication CoVs cytoplasmic Degradation demonstrated develop Diseases drug E3 ubiquitin ligase eight Gene Expression help Host host protein Human IFN increase inhibit inhibiting interact isoform mRNA stability N protein nucleocapsid PABPC4 pathway Protein receptor recruited regulated Replication restriction factor resulting selective selective autophagy severe disease signaling pathway significantly SP1 suppresse the Coronaviridae the interferon the N protein Therapies These data transcription factor SP1 translation Vaccine viral infection viral proliferation viral replication virus [DOI] 10.1128/Spectrum.00908-21 PMC 바로가기 [Article Type] Research Article
Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of CoronavirusesArticle Published on 2021-09-222022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] acute respiratory syndrome addition analyses antibodies antigenic can be used Complete conducted coronavirus Coverage COVID-19 cross-reactivity Diagnosis exhibiting facilitate functional Infection mAb mAbs Middle East monoclonal antibody Mutation N protein neutralization Neutralizing activity neutralizing epitope nucleocapsid nucleocapsid protein Partial property Protein Research responsible S-RBD SARS-CoV SARS-CoV N protein SARS-CoV-2 SARS-CoV-2 N protein specific antibody specificity Spike protein spike receptor-binding domain syndrome tested the N protein the RBD the S protein the SARS-CoV-2 to define Treatment unique variants of concern virus virus replication were assessed [DOI] 10.3390/v13101899 PMC 바로가기 [Article Type] Article
Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19광범위하게 지시된 SARS-CoV-2-특이적 CD4+ T 세포 반응에는 급성 및 소실된 COVID-19 환자의 막 및 핵단백질 내에서 자주 검출되는 펩티드 특이성이 포함됩니다.Research Article Published on 2021-09-162022-09-10 Journal: PLoS Pathogens [Category] SARS, 치료기술, 치료법, 치료제, [키워드] assays Author average binding affinity breadth CD4+ T cell Cohort cohort of patient COVID-19 cytokine data set defined dominant elicited ELISPOT ENCODE enzyme epitope Epitopes Ex vivo examined FIVE Frequency HLA HLA binding HLA class II ICS IFN-γ immune response immune responses immunological Immunopathogenesis in vitro include individual individual immune response interferon interferon-γ intracellular cytokine staining M protein membrane MHC class N protein novel nucleoprotein one patient overlapping Patient patients peptide peptides phenotype Protein resolved response response frequency SARS-CoV-2 envelope SARS-CoV-2 genome SARS-COV-2 infection screened Specificities specificity Spike protein structural protein structural proteins subsequent T cell T cell epitope T cell response T cell responses T cells tetramer the N protein the SARS-CoV-2 the spike protein to define Vaccine design Viral Viral proteins vitro cultivation [DOI] 10.1371/journal.ppat.1009842 PMC 바로가기 [Article Type] Research Article
Interactome Analysis of the Nucleocapsid Protein of SARS-CoV-2 VirusArticle Published on 2021-09-082022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Affinity purification Analysis antiviral strategy binding biogenesis calu-3 cells caused cellular complex damaged EIF2AK2 functions G3BP1 global pandemic HEK293T human cell human cells Infection interaction partner interactome MOV10 multifunctional N protein nucleocapsid protein Pathogenesis Protein public health responses ribonucleocapsid ribonucleoprotein ribosome biogenesis RNA SARS-CoV-2 SARS-COV-2 infection small subunit the N protein the SARS-CoV-2 Transcription translation TRIM25 viral genome replication viral–host interactome virus ZC3HAV1 ZCCHC3 [DOI] 10.3390/pathogens10091155 PMC 바로가기 [Article Type] Article
Targeted in situ cross-linking mass spectrometry and integrative modeling reveal the architectures of three proteins from SARS-CoV-2408 Published on 2021-08-242022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, SARS, [키워드] 40S acute respiratory syndrome application applied approach approaches atomic average binding site Cell cellular complex coronavirus determine domain Effectiveness full-length full-length protein generate highlight in-cell techniques information integrative modeling interactions mass spectrometry methodology Microscopy nucleocapsid nucleocapsid protein occur probe Protein Regulation residues reveal RNA SARS-CoV-2 sequence Significance structural biology Structure structures the N protein Topology Viral protein [DOI] 10.1073/pnas.2103554118 PMC 바로가기 [Article Type] 408
SARS-CoV-2 Proteins Bind to Hemoglobin and Its MetabolitesSARS-CoV-2 단백질은 헤모글로빈과 그 대사산물에 결합합니다Article Published on 2021-08-212022-09-10 Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Adsorption affinity column Analysis analyzed approaches bind binding caused coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 domain explain FIVE hematological heme hemin hemoglobin identify in silico analysis in vitro inhibitory effect Interaction M metabolite Microarray motif motifs N N protein N-terminal domain Nsp3 Nsp7 NTD nucleoprotein peptide peptides Plaque assay Protein protein–protein binding Proteins proteomic Proteomic analysis proteomics protoporphyrin RBD RBD region receptor recruit recruitment Replication respiratory S protein SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike sequence severe acute respiratory syndrome Coronavirus spike Spike protein suppressed the N protein the RBD the S protein the spike protein these compound these compounds Vero cells Viral viral replication virus was used [DOI] 10.3390/ijms22169035 PMC 바로가기 [Article Type] Article
Systematic profiling of SARS-CoV-2-specific IgG responses elicited by an inactivated virus vaccine identifies peptides and proteins for predicting vaccination efficacyArticle Published on 2021-08-172022-10-04 Journal: Cell Discovery [Category] 바이오마커, 비임상, 진단, [키워드] Antibody Response applied BBIBP-CorV China collected convalescent correlated COVID-19 COVID-19 patient Effectiveness elicited facilitate hospitalized COVID-19 patients Humoral response identify immunized immunology inactivated individual Microarray neutralization titer Nsp7 peptide Potential biomarker profile protective mechanism Protein Proteomic analysis response SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 vaccines SARS-CoV-2-specific IgG serum sample the microarray the N protein the spike protein the vaccine triggered vaccination vaccination efficacy Vaccine Vaccines virus Volunteer [DOI] 10.1038/s41421-021-00309-7 PMC 바로가기 [Article Type] Article
Case Report: A Re-Positive Case of SARS-CoV-2 Associated With GlaucomaImmunology Published on 2021-07-282022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] abnormality acute respiratory syndrome antibody Aqueous humor association caused clinical manifestations Complication coronavirus coronavirus disease COVID-19 diagnosed discharged female Glaucoma hospital immunostaining impairment Infection ocular pandemic Patient reported S protein SARS-CoV-2 survived the N protein the patient upper respiratory tract with COVID-19 [DOI] 10.3389/fimmu.2021.701295 PMC 바로가기 [Article Type] Immunology