The C-Terminal Half of SARS-CoV-2 Nucleocapsid Protein, Industrially Produced in Plants, Is Valid as Antigen in COVID-19 Serological TestsPlant Science Published on 2021-07-272022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] Affect antibody Antigen biofactories calculated city coronavirus disease Council COVID-19 COVID-19 diagnosis develop Diagnosis diagnostic diagnostic test diagnostic tests ELISA ELISA coronavirus enzyme-linked immunosorbent expressed expression human sera individual Laboratory molecular farming N antigen N protein Nicotiana benthamiana pandemic plant plant expression plants platform positive result producing protein antigen purification reagent Recombinant protein Research Result RT-PCR SARS CoV-2 nucleocapsid protein SARS-CoV-2 SARS-CoV-2 antibody sensitivity Sensitivity and specificity sera serological shown specificity subset Support Test tested the N protein turnaround times was used were used [DOI] 10.3389/fpls.2021.699665 PMC 바로가기 [Article Type] Plant Science
SARS-CoV-2 N Protein Targets TRIM25-Mediated RIG-I Activation to Suppress Innate ImmunitySARS-COV-2 N 단백질은 타고난 면역을 억제하기 위해 TRIM25- 매개 RIG-I 활성화를 표적으로합니다.Article Published on 2021-07-232022-09-10 Journal: Viruses [Category] SARS, 유전자 메커니즘, 치료법, [키워드] accessory proteins Activation Affect affecting antagonistic characterized clinical picture contributing to counteract COVID-19 Cytokines decrease demonstrated domain ENCODE exacerbated expressed feature host immune system IFN Infection Innate innate immune response Innate immunity Interaction involved Mask mechanism molecular mechanism molecular mechanisms N protein novel SARS-CoV-2 virus occurred Pathways Pattern recognition pattern recognition receptor pro-inflammatory cytokine pro-inflammatory cytokines Protein PRR Recognition reported RIG-I RIG-I activation SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 N protein SARS-CoV-2 virus secretion signaling pathways suppresse target the N protein the SARS-CoV-2 TRIM25 Viral viral pathogenesis [DOI] 10.3390/v13081439 PMC 바로가기 [Article Type] Article
Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNARNA에 부분적으로 무질서한 SARS-CoV-2 뉴클레오캡시드 인단백질 이량체의 다가 결합Article Published on 2021-07-202022-09-11 Journal: Biophysical Journal [Category] SARS, 치료기술, 치료법, [키워드] acute respiratory syndrome aggregation autoproteolysis bind binding biochemical technique Combination complex complexes core coronavirus Critical dimeric disorder domain experiment experiments explain fragment of heterogeneous identify Infection infection cycle infective interact Interaction isothermal titration calorimetry linkers maturation membrane multivalent N protein N proteins NMR nucleocapsid Nucleocapsid phosphoprotein nucleotide oligomers protease Proteases PROTECT Protein purity Replication RNA SARS-CoV-2 nucleocapsid scattering severe acute respiratory syndrome Coronavirus Spike protein susceptibility the N protein ultracentrifugation Viral RNA virion virus [DOI] 10.1016/j.bpj.2021.03.023 PMC 바로가기 [Article Type] Article
Electrostatic features for nucleocapsid proteins of SARS-CoV and SARS-CoV-2SARS-CoV 및 SARS-CoV-2의 뉴클레오캡시드 단백질에 대한 정전기적 특성Article Published on 2021-07-022022-09-10 Journal: Mathematical biosciences and engineering : MBE [Category] SARS, 치료법, [키워드] Acidic acute respiratory syndrome affecting best binding binding energy Computational approaches coronavirus COVID-19 DelPhi DelPhiForce develop directions electrostatic force feature function Health identity increasingly mechanism N protein N proteins nucleocapsid nucleocapsid protein positive Protein protein-protein interactions protein-RNA/DNA interactions respiratory RNA RNAs SARS-CoV SARS-CoV N protein SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spread stability structures the N protein the SARS-CoV-2 treatments for COVID-19 understanding [DOI] 10.3934/mbe.2021120 PMC 바로가기 [Article Type] Article
Structural and conformational analysis of SARS CoV 2 N-CTD revealing monomeric and dimeric active sites during the RNA-binding and stabilization: Insights towards potential inhibitors for N-CTDRNA- 결합 및 안정화 동안 SARS COV 2 N-CTD의 구조적 및 구조적 분석 단량체 및 이량 체 활성 부위를 나타냅니다. N-CTD에 대한 잠재적 억제제에 대한 통찰력Article Published on 2021-07-012022-08-31 Journal: Computers in biology and medicine [Category] SARS, 치료법, 치료제, [키워드] active site active sites acute respiratory syndrome acute respiratory syndrome coronavirus advent Analysis Antiviral binding binding site boceprevir C terminal C-terminal domain Cefoperazone Ceftaroline Ceftaroline fosamil complex conformational conformational change coronavirus correlated CTD Degradation dimer dimeric docking domain drug dynamic Dynamic cross-correlation matrix (DCCM) exonuclease explain FDA-approved drug FDA-approved drugs fluctuation genomic RNA Health help Host hosts independent inhibitor insight involved leads molecular molecular dynamics Molecular dynamics (MD) Molecular mechanics Molecular mechanics/generalized born model and solvent accessibility (MM/GBSA) monomer monomeric N protein N-terminal domain NTD nucleases nucleocapsid Nucleocapsid C terminal domain (N-CTD) PC1 PCA Prevent Protease inhibitor PROTECT Protein protein-ligand interaction residue respiratory ribonucleoprotein RMSD RMSF RNA SARS CoV SARS-CoV-2 Schrodinger severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) solvent stability subunit the N protein Viral viral genome viral genomic RNA Virtual screening virus [DOI] 10.1016/j.compbiomed.2021.104495 PMC 바로가기 [Article Type] Article
Diverse Humoral Immune Responses in Younger and Older Adult COVID-19 PatientsResearch Article Published on 2021-06-292022-10-29 Journal: mBio [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome age Amino acid antibody antibody reactivity Antibody Response antigenic B.1.1.7 B.1.526 B1.351 CD40 ligand chemokine clinical outcomes Clinical severity clinical variables Cohort collected coronavirus Coronaviruses correlation COVID-19 cytokine Cytokine analysis EGF elevated Endemic Endemic coronaviruses epidermal epitope Epitopes Frame group growth factor HCoV-NL63 HCoV-OC43 human coronavirus OC43 Humoral immunity IgG antibody IL-10 IL-15 in vitro transcription individual interferon IP-10 mechanically ventilated membrane MERS-CoV Microarray Middle East Mutation nucleocapsid Older Older age older patient ORF overlap overlapping P.1 Patient patients probe profiles Protein Proteins Region respiratory syndrome coronavirus response SARS-CoV SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 spike SARS-CoV-2 variant selected serum sample significantly significantly higher subset systemic cytokine the N protein the S1 protein translation Ventilated Viral variants [DOI] 10.1128/mBio.01229-21 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 outbreak in a synagogue community: longevity and strength of anti-SARS-CoV-2 IgG responses회당 커뮤니티에서 SARS-CoV-2 발생: 항 SARS-CoV-2 IgG 반응의 수명과 강도Original Paper Published on 2021-06-242022-09-11 Journal: Epidemiology and Infection [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] (Abbott Abbott absence addition anti-SARS-CoV-2 IgG antibodies Antibody Response Architect asymptomatic infections Chain Reaction Community DiaSorin effort ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay herd immunity high correlation IgG IgG antibodies IgG responses Immunity indicate individual Local Longevity MOST N protein neutralisation Neutralising Antibodies outbreak pandemic participant Patient PCR polymerase chain polymerase chain reaction positive Receptor binding domain respiratory response S protein SARS-CoV-2 Sero-prevalence Serological assay serological assays seropositive Severe acute respiratory syndrome severe acute respiratory syndrome-coronavirus-2 strength symptomatic individual symptomatic individuals the N protein the S protein vaccination [DOI] 10.1017/S0950268821001369 PMC 바로가기 [Article Type] Original Paper
The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1AMicrobiology Published on 2021-06-232022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Actin acute respiratory syndrome beta coronavirus C-terminal contribute coronavirus Coronavirus infections cytokinesis domain EF1A F-actin highlight Human immunoprecipitation in vitro inactive Interaction involved MERS-CoV MERS-CoV infection MERS-CoV N MERS-CoV-infected cells Middle East motif mRNA multiple nuclei nucleocapsid nucleocapsid protein pathogen Pathogenesis Protein protein translation provide reporter respiratory syndrome coronavirus SARS-CoV severe respiratory disease significantly the N protein translation Viral protein [DOI] 10.3389/fmicb.2021.551602 PMC 바로가기 [Article Type] Microbiology
A Comparative Analysis of Coronavirus Nucleocapsid (N) Proteins Reveals the SADS-CoV N Protein Antagonizes IFN-β Production by Inducing Ubiquitination of RIG-IImmunology Published on 2021-06-162022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, MERS, SARS, [키워드] acute diarrhea acute respiratory syndrome Analysis binding activity caused claimed Comparative conducted coronavirus CoV CoV infection COVID-19 pandemic CoVs Degradation Delay epidemic diarrhea Host IBV IFN IFN response IFN-β independent Inducing infected cell Infectious Bronchitis Virus inhibiting Interaction interferon MERS-CoV Middle East nucleocapsid nucleocapsid protein offer Porcine deltacoronavirus Production Protein retinoic acid-inducible gene I (RIG-I) RIG-I RNA SARS-CoV SARS-CoV-2 Severity of disease subsequent suppress swine acute diarrhea syndrome coronavirus (SADS-CoV) syndrome the N protein therapy ubiquitination Viral protein viruses [DOI] 10.3389/fimmu.2021.688758 PMC 바로가기 [Article Type] Immunology
The SARS-CoV-2 Nucleocapsid Protein and Its Role in Viral Structure, Biological Functions, and a Potential Target for Drug or Vaccine MitigationReview Published on 2021-06-102022-10-28 Journal: Viruses [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Biological biological function Biological functions component coronavirus drug drug target functional homology knowledge Like Mitigation N protein nucleocapsid nucleocapsid protein other coronaviruses Potential Protein role SARS-CoV-2 Spread Structure target the N protein the SARS-CoV-2 Vaccine vaccine candidate virus [DOI] 10.3390/v13061115 PMC 바로가기 [Article Type] Review