The SARS-CoV-2 Spike Glycoprotein Biosynthesis, Structure, Function, and Antigenicity: Implications for the Design of Spike-Based Vaccine ImmunogensImmunology Published on 2020-10-072022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome adaptive antigenicity Biosynthesis caused coronavirus coronavirus disease COVID-19 ENCODE function global public health highlight host cell host immune responses Immunity immunogen immunogen design in viral Infection Like membrane fusion Neutralizing antibodies other coronaviruses pandemic Receptor-binding domain S glycoprotein S protein-based vaccine SARS-CoV-2 spike spike glycoprotein Structure surface synthesis the S protein the SARS-CoV-2 the SARS-CoV-2 genome therapeutic interventions Vaccine virions virus [DOI] 10.3389/fimmu.2020.576622 PMC 바로가기 [Article Type] Immunology
SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification530 Published on 2020-09-292022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, SARS, [키워드] acute respiratory syndrome coronavirus diagnostic diagnostic capabilities downstream Effect equipment expand help inactivation isothermal isothermal amplification nucleases pandemic performed platform precautions protocol purification reagents RNA RT-LAMP SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 testing sensitivity Significance stability variety viral inactivation Viral RNA virions [DOI] 10.1073/pnas.2011221117 PMC 바로가기 [Article Type] 530
A molecular pore spans the double membrane of the coronavirus replication organelleReport Published on 2020-09-112022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, [키워드] Antiviral cellular complex coronavirus Coronavirus replication cytosol cytosolic drug target Genome genome replication host cell membrane identify infected cell membrane Messenger RNA microenvironment Microscopy molecular organelle progeny virions remained Replication RNA Science sealed thought translation transmembrane protein Travel Vesicle vesicles viral genome replication Viral RNA viral RNA synthesis virions Wolff [DOI] 10.1126/science.abd3629 PMC 바로가기 [Article Type] Report
Air filtration and SARS-CoV-2Covid-19 Published on 2020-07-042022-10-31 Journal: Epidemiology and Health [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Aerosols anticipated Concentration coronavirus coronavirus disease COVID-19 COVID-19 pandemic Critical determine effective Effectiveness equipment Evidence filtration HEPA implementation Intervention less management Masks mechanism Misconception MPPS Other Particle Particles reduce Removing respiratory droplet SARS-CoV-2 SARS-CoV-2 virion shown Spread spread of COVID-19 Travel viability virions while [DOI] 10.4178/epih.e2020049 PMC 바로가기 [Article Type] Covid-19
Neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalizationResearch Published on 2018-08-302022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] 2B11 antibody attachment B cell binding blocked cause Cell conformational defined epidemic diarrhea epitope expression generate host cell receptor identify Infection inhibit inhibited Isolating mAb mechanism monoclonal antibody neutralized neutralizing antibody pathogenic coronavirus PEDV Porcine epidemic diarrhea virus prokaryotic expression system qPCR receptor-binding domains Result S protein secrete specific antibodies suggested supplementary material Surface glycoprotein target protein the receptor-binding domain Vero cells Vero E6 cell viral spike virions virus virus entry virus infection was performed was used were used [DOI] 10.1186/s12985-018-1042-3 PMC 바로가기 [Article Type] Research
Selective Packaging in Murine Coronavirus Promotes Virulence by Limiting Type I Interferon ResponsesResearch Article Published on 2018-05-012022-10-29 Journal: mBio [Category] Coronavirus, MERS, [키워드] absence antibodies Betacoronavirus caused cellular coronavirus CoV CoVs cultured cells Effect genomic genomic RNA gRNA IFN IFNAR in viral in vitro in vivo increases in Infection innate immune innate immune response innate immune system interferon interferon response interferon signaling locus marrow-derived macrophage MAVS mechanism MHV mice murine hepatitis virus mutant mutant virus Mutation not affect Nsp15 ORF1b packaged packaging signal Pathogenesis pathogenicity positive positive-sense promote receptor reduced remained replicated Replication required response restored RNA RNA molecule RNA packaging RNAs selective selective packaging significantly strain subgenomic RNAs These data TLR7 treated Type virion virions virulence virus virus replication viruses weight loss [DOI] 10.1128/mBio.00272-18 PMC 바로가기 [Article Type] Research Article
Temporary carriage of bovine coronavirus and bovine respiratory syncytial virus by fomites and human nasal mucosa after exposure to infected calvesResearch Article Published on 2018-01-222022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] biosecurity bovine Bovine coronavirus Bovine respiratory disease cattle Cell culture collected Contact determine Endemic equipment experiments high viral load Human nasal mucosa Indirect transmission Infection infective knowledge mucosal swab nasal mucosa pathogen positive Prevent quantified respiratory syncytial virus Result risk RNA RT-PCR RT-qPCR selected short-lived Spread Swab swabs temporary tested Transmission transmission potential viral particle Viral RNA viral RNA load virion virions virus virus infectivity viruses [DOI] 10.1186/s12917-018-1335-1 PMC 바로가기 [Article Type] Research Article
Within-Host Spatiotemporal Dynamics of Plant Virus Infection at the Cellular Level세포 수준에서 식물 바이러스 감염의 숙주 내 시공간 역학Research Article Published on 2014-02-272022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] aggregation animal hosts appearance approach Cell cells cellular colonize complex Contagion described determine Disease progression Dynamics estimate flask flow cytometry Frequency Host individual cell individual cells infect infected cell infected cells infecting Infection initiated leaf Level modeling MOI monolayer multicellular multicellular organism multiplicity of infection not significantly different observation organism pattern per day plant plant virus plants ranged replicate Secondary infection secondary infections Space Spread Symptoms technique variance virion virions virus virus expansion virus infection virus particle virus titer virus titers virus variant virus-infected cell virus-infected cells viruses [DOI] 10.1371/journal.pgen.1004186 PMC 바로가기 [Article Type] Research Article
The Coronavirus E Protein: Assembly and BeyondReview Published on 2012-03-082022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] assembly coronavirus coronavirus assembly E protein envelope protein function Golgi complex highlight Host host proteins indicated Infection interact ion channel membrane membrane protein membrane protein topology recent related Stress topologies virions virus [DOI] 10.3390/v4030363 PMC 바로가기 [Article Type] Review