supernatant – Page 6 – Communicable Diseases Research

Inactivation of Material from SARS-CoV-2-Infected Primary Airway Epithelial Cell Cultures

Letter

Published on 2021-01-072022-10-30
Journal: Methods and Protocols [Category] COVID-19,

[키워드] acute respiratory syndrome AEC airway airway epithelium BSL-3 BSL-3 laboratory CDC Cell Cell culture center conducted Control coronavirus COVID-19 Culture downstream Effectiveness epithelial epithelial cell evaluated experiment facilitate Factor fixation fixed formalin Guidance heat Heating immunohistochemistry inactivated inactivation Infection initial innate immune response Isolation Laboratory level 3 live SARS-CoV-2 material prevention primary human airway Protein protocol protocols RNA Safe SARS-CoV-2 SDS sodium supernatant tested transfer Treatment Triton X100 ultraviolet UV light Vero E6 Vero E6 cell viral entry virus
[DOI] 10.3390/mps4010007 PMC 바로가기 [Article Type] Letter

High Induction of IL-6 Secretion From hUCMSCs Optimize the Potential of hUCMSCs and TCZ as Therapy for COVID-19-Related ARDS

hUCMSC에서 IL-6 분비의 높은 유도는 COVID-19 관련 ARDS에 대한 치료법으로서 hUCMSC 및 TCZ의 잠재력을 최적화합니다

Article

Published on 2021-01-012022-09-12
Journal: Cell transplantation [Category] 진단,

[키워드] acute respiratory distress analyzed anti-inflammatory effect ARDS Biological Blood Cell cellular Concentration correlation COVID-19 cytokine secretion ELISA evaluated Exogenous expression functional hUCMSC Hyperinflammatory syndrome IL-6 IL-6 level IL-6 levels IL-6 neutralizing antibody IL-6 receptor IL-6R induction Inflammation inhibited inhibitory effect interleukin-6 less Microarray mononuclear cell mRNA expression MTT Neutralizing neutralizing antibody PBMC PBMCs peripheral Potential proliferation reached reported Respiratory failure Responder Severe COVID-19 pneumonia supernatant syndrome TCZ therapeutic potential therapy Tocilizumab treat umbilical was measured was used
[DOI] 10.1177/09636897211054481 PMC 바로가기 [Article Type] Article

SARS-CoV-2 infects and induces cytotoxic effects in human cardiomyocytes

SARS-CoV-2는 인간 심근세포를 감염시키고 세포독성 효과를 유도합니다

Article

Published on 2020-12-012022-09-11
Journal: Cardiovascular Research [Category] MERS, SARS, 진단, 치료기술, 치료법, 치료제,

[키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 antiviral drug Apoptosis blocked Caco-2 cardiac injury cardiomyocyte Cardiosphere Cardiotoxicity cathepsin cathepsin-dependent Cathepsins caused cell lines Concentration coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cytotoxic effect demonstrated dependent on direct infection double-stranded Effect elevated enzyme expression global pandemic glycoprotein heart Heart slice higher risk in vitro Increasing induce infect Infection infection with SARS-CoV-2 infections Inflammation inhibited Injury interferon lead Lung injury marker Mortality oxygen Particle pathway Pathways Patient reactive oxygen Remdesivir respiratory RNA sequencing SARS-CoV-2 SARS-COV-2 infection secondary to severe acute respiratory syndrome Coronavirus spike glycoprotein Spike protein strain Stress supernatant supernatants tissue transcriptional response up-regulation Viral viral infection viral particle Viral particles Viral RNA viral spike viral spike protein
[DOI] 10.1093/cvr/cvaa267 PMC 바로가기 [Article Type] Article

Nano-curcumin therapy, a promising method in modulating inflammatory cytokines in COVID-19 patients

Randomized Controlled Trial

Published on 2020-12-012022-10-05
Journal: International immunopharmacology [Category] SARS, 임상,

[키워드] acute respiratory distress Anti-inflammatory approach ARDS complications Concentration Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients cytokine secretion Cytokine storm disorder Effect ELISA evaluated expression Gene Expression Health healthy control identify IL-18 IL-1β IL-6 IL-6 expression indicated Inflammatory cytokine inflammatory cytokine production modulate modulating modulation mRNA expression multi-organ failure Nano-curcumin. Patient PCR Pneumonia receiving recruited secretion serum significant decrease significantly supernatant syndrome the placebo group therapy TNF-α Treatment were assessed
[DOI] 10.1016/j.intimp.2020.107088 PMC 바로가기 [Article Type] Randomized Controlled Trial

Data, Reagents, Assays and Merits of Proteomics for SARS-CoV-2 Research and Testing

Research

Published on 2020-11-252022-10-30
Journal: Molecular & Cellular Proteomics : MCP [Category] COVID-19, SARS,

[키워드] A549 ACE2 African green monkey Analysis approach BRCA1 Breast cancer Caco-2 Calu-3 can be used cause cell line change changed Chlorocebus clinical proteomics Cohort COVID-19 COVID-19 pandemic COVID-19 research diagnostic diagnostics evaluate Evidence expression expression data facilitate Factor feasibility genomes globe Innate immunity involved kDa label-free quantification manuscript mass spectrometry monitoring parallel reaction monitoring peer-reviewed peptides positive profile protease inhibitors Protein protein expression Proteins proteome proteomic proteomics Region representing Research SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 proteins SARS-CoV-2 research Spread SRP9 stable-isotope labeling supernatant synthetic synthetic peptide targeted mass spectrometry Testing TMPRSS2 transcriptional regulators Vero Cell Vero E6 Vero E6 cell viral entry viral infection virus widespread
[DOI] 10.1074/mcp.RA120.002164 PMC 바로가기 [Article Type] Research

Direct activation of the alternative complement pathway by SARS-CoV-2 spike proteins is blocked by factor D inhibition

Article

Published on 2020-10-292022-10-06
Journal: Blood [Category] SARS, 진단,

[키워드] activate Activation acute respiratory syndrome Alternative pathway animal antibody APC Atypical blocked C3c catastrophic cells clinical manifestation complement Complement pathway contagious coronavirus COVID-19 Direct disease end-organ end-organ damage endothelial damage Evidence explain Human Hypothesis in vitro inactivation Injury killing mechanism mice mitigate Myocardial infarction Prevent renal respiratory virus SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 spike proteins Spike protein stroke subunit supernatant syndrome target cells the N protein the SARS-CoV-2 thrombosis Trigger upstream uremic syndrome
[DOI] 10.1182/blood.2020008248 PMC 바로가기 [Article Type] Article

Potential of Cell-Free Supernatant from Lactobacillus plantarum NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants

Article

Published on 2020-09-232022-10-28
Journal: Pharmaceuticals [Category] COVID-19,

Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation

Short Communication

Published on 2020-09-232022-11-01
Journal: Journal of virological methods [Category] COVID-19, MERS, SARS,

Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?

Article

Published on 2020-07-072022-10-28
Journal: Viruses [Category] COVID-19, SARS,

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Brief Report

Published on 2020-06-082022-10-28
Journal: Viruses [Category] COVID-19, SARS,