A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patientsCOVID-19 환자에서 항-스파이크 항체의 혈청 학적 검출을위한 유세포 분석법 분석Protocol Published on 2021-06-192022-09-01 Journal: STAR protocols [Category] MERS, SARS, 진단, 치료기술, [키워드] Abstract anti-spike antibody antibody capture Cell Cell Biology cell surface Cloning complement Complete coronavirus coronavirus 2019 disease COVID-19 COVID-19 patient Cytometry detect Early detection eGFP Express Flow flow cytometry Flow Cytometry/Mass Cytometry full-length SARS-CoV-2 full-length SARS-CoV-2 spike protein Health Sciences immunology infected individual infected individuals insertion limit management Molecular biology patients with COVID-19 PCR-based assays Plasmid protocol public health S protein SARS-CoV-2 spike SARS-CoV-2 spike protein serological Serological assay serological assays specific antibodies specific antibody Spike protein the cell the S protein transduced transfection transfer Transmission Viral viral particle Viral particles [DOI] 10.1016/j.xpro.2021.100671 PMC 바로가기 [Article Type] Protocol
Cross-Reactivity of Two SARS-CoV-2 Serological Assays in a Setting Where Malaria Is Endemic말라리아가 풍토병인 환경에서 두 SARS-CoV-2 혈청학적 분석의 교차 반응성Article Published on 2021-06-182022-09-11 Journal: Journal of Clinical Microbiology [Category] MERS, SARS, 말라리아, 진단, 치료법, [키워드] Abbott alleviate antibody Antibody binding Architect assays association avidity Concentration COVID-19 Critical cross-reactivity Endemic Euroimmun False positive False positives IgG IgG level indicated malaria moderate Nigeria PCR PCR-positive plasma Plasmodium Plasmodium falciparum Plasmodium falciparum infection positive Previous studies previous study Protein reduced SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 patient SARS-CoV-2 patients Serological assay serological assays serology setting significantly higher tested urea urea concentration urea concentrations was performed WASH [DOI] 10.1128/JCM.00514-21 PMC 바로가기 [Article Type] Article
Glycan reactive anti-HIV-1 antibodies bind the SARS-CoV-2 spike protein but do not block viral entryArticle Published on 2021-06-142022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, SARS, [키워드] ADE antibodies antibody Antibody titer Antibody-dependent enhancement Antigen binding block viral entry contribute COVID-19 Critical cross-reactive antibody cross-reactivity displaying ELISA experiment expressing glycan glycosylation gp120 HIV-1 immune response immunoassays immunoreactivity implication Infection interfere Interpretation Microbiology motif Neutralizing nine pseudovirus reactive receptor engagement SARS-CoV-2 SARS-CoV-2 S SARS-CoV-2 spike glycoprotein SARS-CoV-2 spike protein selected serological assays Spike protein Spike proteins the SARS-CoV-2 therapeutic unlikely vaccine immunogen Virology virus Virus neutralization [DOI] 10.1038/s41598-021-91746-7 PMC 바로가기 [Article Type] Article
Longitudinal detection of SARS‐CoV‐2‐specific antibody responses with different serological methods다양한 혈청학적 방법을 통한 SARS-CoV-2 특이 항체 반응의 종단적 검출Comparative Study Published on 2021-06-112022-09-10 Journal: Journal of medical virology [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus analyzed antibodies antibody Antibody Response Antigen antigens assays Blood donors cells children collected Concentration coronavirus coronavirus disease Coronavirus disease 2019 COVID‐19 COVID‐19 detect diagnostic eight ELISA ELISAs healthy blood donors highest IgA IgG IgM immunofluorescence Immunoglobulin immunoglobulin A Immunoglobulin G immunoglobulin M immunosorbent indirect immunofluorescence individual infections information Kinetics longitudinal Patient peaked polymerase chain polymerase chain reaction Prevalence Protein provided respiratory S1 domain SARS‐CoV‐2 SARS‐CoV‐2‐infected SARS‐CoV‐2 seasonal coronaviruses selected sensitivities sensitivity sera serological serological assays Serological testing serology serum sample serum samples specific antibodies specific antibody specificity Spike protein substrate symptom onset tested the viral nucleocapsid Viral viral nucleocapsid protein virus [DOI] 10.1002/jmv.27113 PMC 바로가기 [Article Type] Comparative Study
Validation of high-throughput, semiquantitative solid phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices혈청 및 건조 혈액 반점 매트릭스에서 고 처리량, 반 정량적 고체 상자 SARS 코로나 바이러스 -2 혈청학 분석의 검증Research Article Published on 2021-06-112022-09-01 Journal: Bioanalysis [Category] MERS, SARS, 진단, 치료기술, [키워드] antibody Blood collected convalescent correlated COVID-19 demonstrated diagnostic diagnostic specificity Donor dried blood spot Efficacy ELISA global pandemic IgA IgG IgM Infection Ligand NAb neutralization Neutralizing antibodies neutralizing antibody pseudotype R 2 SARS-CoV-2 sensitivity serological Serological assay serological assays serology serology assay serum serum IgG solid phase specificity Surveillance Trajan Vaccine validation Victoria [DOI] 10.4155/bio-2021-0065 PMC 바로가기 [Article Type] Research Article
A systematic and meta-analysis review on the diagnostic accuracy of antibodies in the serological diagnosis of COVID-19COVID-19의 혈청학적 진단에서 항체의 진단 정확도에 대한 체계적이고 메타분석적 검토Review Published on 2021-05-262022-09-01 Journal: Systematic Reviews [Category] SARS, 진단, 치료기술, [키워드] Accuracy alternative method antibody best carried characteristic chemiluminescence CLIA Complete COVID-19 detect diagnose diagnosis of COVID-19 diagnostic Diagnostic accuracy diagnostic test eligible ELISA ELISA test enzyme enzyme immunoassay evaluate evaluated except for expressed gold gold standard heterogeneity Hierarchical highest IgG IgG and IgM IgM immunoassay Infection lateral flow immunoassay LFIA LFIA test medRxiv Meta-analysis Patient performed POC point-of-care proportion QUADAS-2 receiver operating characteristic Research Result rRT-PCR SARS-CoV-2 SARS-CoV-2 antigens SARS-CoV2 sensitivity serological Serological assay serological assays Serological tests serology specificity supplementary material systematic literature search was performed was used [DOI] 10.1186/s13643-021-01689-3 PMC 바로가기 [Article Type] Review
False Positive Results in SARS-CoV-2 Serological Tests for Samples From Patients With Chronic Inflammatory Diseases만성 염증 질환 환자의 샘플에 대한 SARS-CoV-2 혈청 검사의 위양성 결과Immunology Published on 2021-05-032022-09-11 Journal: Frontiers in Immunology [Category] SARS, 진단, 치료기술, [키워드] analyzed antibody Arthritis autoantibodies Autoimmunity chronic chronic inflammatory disease chronic inflammatory diseases collected defined diagnostics ELISA kit evaluate False positive False positivity house IgG Immunity Immunosuppressant immunosuppressants IMPROVE Infection Inflammatory diseases lateral flow lupus erythematosus majority multiple sclerosis multiplex notable pandemic Patient patients peptide Poor positive produced Result Rheumatoid rheumatoid arthritis rheumatoid factor risk Sample SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 pandemic Serological assay serological assays serological test Serological tests SLE specificity systemic lupus erythematosus Test the SARS-CoV-2 treated [DOI] 10.3389/fimmu.2021.666114 PMC 바로가기 [Article Type] Immunology
Evaluation of High-Throughput SARS-CoV-2 Serological Assays in a Longitudinal Cohort of Patients with Mild COVID-19: Clinical Sensitivity, Specificity, and Association with Virus Neutralization Test경증 COVID-19 환자의 세로 코호트에서 처리량 SARS-CoV-2 혈청학적 분석 평가: 임상 민감도, 특이도 및 바이러스 중화 테스트와의 연관성Article Published on 2021-04-292022-09-11 Journal: Clinical Chemistry [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] Antibody Response Antibody titers Area association AUC best can not clinical clinical sensitivity clinical specificity collected Concordance Course COVID-19 detect DiaSorin disease evaluated except for functional greater health-care Health-care workers healthcare worker IgA IgG IgM longitudinal lowest mild patient mild patients neutralization test Neutralizing antibodies neutralizing antibody neutralizing antibody titers Neutralizing titer ranged RBD Receptor binding domain ROC Curve RT-PCR S protein SARS-CoV-2 sensitivity sera serological Serological assay serological assays Serological tests serum Siemens specificity specimen subset Test the RBD the S protein Total Virus neutralization virus neutralization test VNT with COVID-19 [DOI] 10.1093/clinchem/hvaa336 PMC 바로가기 [Article Type] Article
From Multiplex Serology to Serolomics—A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome다중 혈청학에서 혈청학으로 - SARS-CoV-2 단백질체에 대한 항체 반응에 대한 새로운 접근Article Published on 2021-04-242022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] age Algorithm Analysis Antibody Response Antibody responses assays ccCoVs Cohort common cold common cold Coronaviruses Control Course COVID-19 case COVID-19 patient determine Disease progression dual E. coli ELISA enzyme-linked immunosorbent expressed fluorescent HEK293 cells hospitalized patient Ig isotype Ig isotypes immunofluorescence Immunoglobulin multiplex serology novel pathogen Pathogens Protein proteome Receptor-binding domain Research question response SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 epidemiology SARS-CoV-2 N SARS-CoV-2 pandemic SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV-2 proteome SARS-CoV-2 research sensitivity Sensitivity and specificity Serological assay serological assays specificity Spike protein symptom onset the receptor-binding domain the SARS-CoV-2 the spike protein [DOI] 10.3390/v13050749 PMC 바로가기 [Article Type] Article
Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 DiagnosisArticle Published on 2021-04-212022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] Antibody titers assay performance assays comparable concerning COVID-19 decrease Diagnosis diagnosis of SARS-CoV-2 eight evaluated heterogeneity heterogeneous Hospitalization IgG immune response Infection longitudinal Month Neutralizing Neutralizing antibody titer Neutralizing titer Patient Postinfection RT–PCR SARS-CoV-2 SARS-COV-2 infection sensitivity serological assays serological test serology tested [DOI] 10.3390/jcm10091815 PMC 바로가기 [Article Type] Article