ELISA, enzyme-linked immunosorbent assay – Communicable Diseases Research

Development and comparative evaluation of SARS-CoV-2 S-RBD and N based ELISA tests in various African endemic settings

Article

Published on 2023-01-222024-09-05
Journal: Diagnostic microbiology and infectious disease [Category] update2024,

Performance of the SureScreen Diagnostics COVID-19 antibody rapid test in comparison with three automated immunoassays

Article

Published on 2023-01-102024-09-05
Journal: Diagnostic microbiology and infectious disease [Category] update2024,

[키워드] anti-N, anti-nucleocapsid anti-S, anti-spike CMV, cytomegalovirus COVID-19 EBV, Epstein-Barr virus ELISA, enzyme-linked immunosorbent assay lateral flow immunoassays (LFIA) LFIA, Lateral Flow Immunoassays NTD, N-terminal domain RBD, receptor-binding domain SARS-CoV-2 serology SureScreen Diagnostics
[DOI] 10.1016/j.diagmicrobio.2023.115900 PMC 바로가기 [Article Type] Article

Tofacitinib-Induced Modulation of Intestinal Adaptive and Innate Immunity and Factors Driving Cellular and Systemic Pharmacokinetics

Original Research

Published on 2022-10-062024-09-04
Journal: Cellular and molecular gastroenterology and hepato [Category] 대상포진,

[키워드] 3D, 3-Dimensional ASV, Amplicon sequence variant ATP, adenosine triphosphate CD, Crohn’s disease CFSE, carboxyfluorescein succinimidyl ester DI, division index DSS, dextran sulfate sodium ELISA, enzyme-linked immunosorbent assay ENT, equilibrative nucleoside transporter FSC, forward scatter IBD, Inflammatory bowel disease IEC, intestinal epithelial cell IFNγ, interferon γ IHC, immunohistochemistry IL, interleukin Inflammatory bowel disease JAK inhibitor JAK, Janus kinase JAKi, Janus kinase inhibitor LC-MS/MS, liquid chromatography–tandem mass spectrometry LPS, lipopolysaccharide M-CSF, macrophage colony-stimulating factor mRNA, messenger RNA Mucosal inflammation PBMC, peripheral blood mononuclear cell PBS, phosphate-buffered saline PHA, phytohemagglutinin pharmacokinetics PI, proliferation index STAT, signal transducers and activator of transcription Th, T helper TNF-α, tumor necrosis factor α Tofacitinib UC, ulcerative colitis
[DOI] 10.1016/j.jcmgh.2021.09.004 PMC 바로가기 [Article Type] Original Research

Lack of effects on female fertility or pre- and postnatal development of offspring in rats after exposure to AS03-adjuvanted recombinant plant-derived virus-like particle vaccine candidate for COVID-19

Article

Published on 2021-11-242023-06-15
Journal: Reproductive toxicology (Elmsford, N.Y.) [Category] MERS, SARS,

[키워드] AS, adjuvant system AS03 CI, Confidence interval COVID-19 vaccine COVID-19, Coronavirus disease 2019 CoVLP, coronavirus-like particle CT, cytoplasmic tail DART developmental and reproductive toxicity Developmental and reproductive toxicity study EFD, embryofetal development ELISA, enzyme-linked immunosorbent assay Fertility GD, gestation day GLP, good laboratory practice GMT, geometric mean titer HCD, historical control data HRP, horse-radish peroxidase IM, intramuscular immunogenicity MRD, minimum required dilution OECD, organisation for economic co-operation and development PBS, phosphate buffered saline PND, postnatal day PPN, pre and postnatal Pregnancy QA, quality assurance RBD, receptor binding domain S, spike SARS-CoV-2 SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SD, study day SOP, standard operating procedure TM, transmembrane domainVLP virus-like particle virus-like particles
[DOI] 10.1016/j.reprotox.2021.11.006 PMC 바로가기 [Article Type] Article

Development of a recombinant vaccine containing a spike S1-Fc fusion protein induced protection against MERS-CoV in human DPP4 knockin transgenic mice

Article

Published on 2021-10-302022-11-01
Journal: Journal of virological methods [Category] Coronavirus, MERS,

[키워드] addition alum adjuvant antigen-specific neutralizing antibody baculovirus BALB/c mice BSL, biosafety level CEF, chicken embryo fibroblast cells Chinese hamster CHO, Chinese hamster ovary coronavirus demonstrated development Dipeptidyl peptidase-4 DPP4 effective ELISA, enzyme-linked immunosorbent assay Extracellular family Coronaviridae Fc fragment Fc, fragment of crystalline Fragment of crystalline (Fc) molecule fused fusion protein genus Betacoronavirus highly pathogenic human DPP4 humoral immune response Humoral immunity IgG IgG4 immunogenicity inflammatory cell infiltration inoculated lack MERS-CoV MERS-CoV infection MERS-CoV, Middle East respiratory syndrome coronavirus MERS, Middle East respiratory syndrome mice Middle East Middle East respiratory syndrome Coronavirus Neutralizing antibodies PBS, phosphate-buffered saline PBST, phosphate-buffered saline in 0.05 % Tween 20 PCR, polymerase chain reaction PFU, plaque-forming unit Protective Protein Proteins RBD, receptor-binding domain respiratory syndrome coronavirus RLU, relative light unit S1 subunit SARS-CoV, severe acute respiratory syndrome coronavirus secretion SFM, serum-free medium Spike protein therapeutic transgenic mice Vaccine vaccine candidate Vaccine development viral challenge viral outbreak virus
[DOI] 10.1016/j.jviromet.2021.114347 PMC 바로가기 [Article Type] Article

SARS-CoV-2 spike promotes inflammation and apoptosis through autophagy by ROS-suppressed PI3K/AKT/mTOR signaling

Article

Published on 2021-08-272023-06-16
Journal: Biochimica et biophysica acta. Molecular basis of [Category] MERS, SARS,

Potential therapeutic approaches for the early entry of SARS-CoV-2 by interrupting the interaction between the spike protein on SARS-CoV-2 and angiotensin-converting enzyme 2 (ACE2)

Review

Published on 2021-08-082023-06-15
Journal: Biochemical pharmacology [Category] MERS, SARS,

[키워드] 6-HB, 6-helix bundle AAK1, AP2-associated protein kinase 1 ACE2, angiotensin-converting enzyme 2 AHR, aryl hydrocarbon receptor. angiotensin-converting enzyme 2 cell entry COVID-19 CoVs, coronaviruses DPP4, dipeptidyl peptidase 4 ELISA, enzyme-linked immunosorbent assay HR1, heptad repeat 1 HR2, heptad repeat 2 M^pro, main protease mAb, monoclonal antibody MERS-CoV, Middle East respiratory syndrome coronavirus NSP, non-structural protein NTD, N-terminal domain ORF1a, open reading frame 1a ORF1b, open reading frame 1b PD, peptidase domain PL^pro, Papain-like protease PPI, Protein-Protein Interaction Protein–protein interaction (PPI) inhibitors RBD, receptor binding domain RdRp, RNA-dependent RNA polymerase S protein, spike protein SARS-CoV-2 SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SARS-CoV, severe acute respiratory syndrome coronavirus Spike protein TMPRSS2, Transmembrane Protease Serine 2
[DOI] 10.1016/j.bcp.2021.114724 PMC 바로가기 [Article Type] Review

Current diagnostic approaches to detect two important betacoronaviruses: Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Review

Published on 2021-07-242023-07-01
Journal: Pathology, research and practice [Category] SARS,

[키워드] 5’-UTR, 5’-untranslated region acpcPNA, pyrrolidinyl peptide nucleic acid ALI, Acute Lung Injury ASO, Antisense oligonucleotides BSL, biosafety level CDC, Centers for Disease Control and Prevention clinical CoV, coronavirus COVID-19, Coronavirus disease of 2019 CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats CT, Computed tomography CXR, chest radiography ddNTPs, dideoxynucleotide triphosphates Diagnosis DNA, Deoxyribonucleic acid dPCR, digital polymerase chain reaction EIA, Enzyme immunoassay ELISA, enzyme-linked immunosorbent assay EUA, Emergency Use Authorization FDA, Food and Drug Administration FIPV, feline infectious peritonitis virus Hel, helicase HIV, Human immunodeficiency virus ICU, Intensive care unit IFA, Immunofluorescence assay Laboratory LAMP, Loop-mediated isothermal amplification LoD, limit of detection LOQ, Limit of quantification MALDI-TOF, MassARRAY matrix-assisted laser desorption ionization-time of flight MERS-CoV MERS, Middle East respiratory syndrome MN, Microneutralisation MS, mass spectrometry NP, nucleocapsid protein NSP, Non-structural Proteins ORF, open reading frame OSN-qRT-PCR, One-step single-tube nested (OSN)-qRT-PCR PCR, polymerase chain reaction PFU, Plaque forming units POCT, Point of care testing ppNT, Pseudoparticle neutralization test PRNT, Plaque reduction neutralization test qPCR, Quantitative or real-time polymerase chain reaction qRT-PCR, Real-time reverse transcription polymerase chain reaction RAD, Rapid antigen test radiological RBD, receptor binding domain RdRp, RNA-dependent RNA polymerase RFLP, restriction fragment length polymorphism RNA, Ribonucleic acid RSV, respiratory syncytial virus RT-iiPCR, Reverse transcription-insulated isothermal polymerase chain reaction RT-RPA, Reverse transcription recombinase polymerase amplification SARS-CoV-2 SARS, Severe acute respiratory syndrome SNP, single nucleotide polymorphism TCID, Tissue Culture Infectious Dose TRPMSS2, Type II transmembrane serine protease upE, Regions upstream of gene E VOC, variants of concern VOHC, Variant of High Consequence VOI, variant of interest VUM, variant under monitoring WGS, Whole-genome sequencing WHO, World Health Organization
[DOI] 10.1016/j.prp.2021.153565 PMC 바로가기 [Article Type] Review

Potent antiviral activity of Agrimonia pilosa , Galla rhois , and their components against SARS-CoV-2

Article

Published on 2021-07-232023-07-02
Journal: Bioorganic & Medicinal Chemistry [Category] SARS,

[키워드] Agrimonia pilosa Galla rhois Antiviral agents AP, Agrimonia pilosa APRG64 APRG64, 50% EtOH aqueous extract mixture of AP and RG which ratio is 6 and 4 BSA, bovine serum albumin c.c., column chromatography COVID-19 COVID-19, Coronavirus disease 2019 DENV-2, dengue virus 2 DMEM, Dulbecco’s modified Eagle’s medium ELISA, enzyme-linked immunosorbent assay FBS, fetal bovine serum HCV, hepatitis C virus hpi, hours post-infection HSV-1, Herpes simplex virus type 1 IAV, influenza A virus LC/MS, liquid chromatography/mass spectrometry MEM, Eagle’s Minimum Essential Medium ODS, octadecyl SiO₂ PFU, plaque-forming unit RG, Galla rhois SARS-CoV-2 SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 SEM, standard error of the mean SiO₂, silica gel
[DOI] 10.1016/j.bmc.2021.116329 PMC 바로가기 [Article Type] Article

Focused role of nanoparticles against COVID-19: Diagnosis and treatment

Review

Published on 2021-04-062023-06-15
Journal: Photodiagnosis and photodynamic therapy [Category] MERS, SARS,

[키워드] 2019-nCoV, severe acute respiratory syndrome coronavirus 2 Ag-MES, silver nanoparticle capped with mercaptoethane sulfonate AMT, 4′-aminomethyl-trioxsalene ARDS, acute respiratory distress syndrome AuNP-ab, gold-antibody nanoparticle BAL, bronchoalveolar lavage cDNA, complementary DNA COVID-19 COVID-19, coronavirus disease CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats CT, Computed tomography Diagnosis EHEC, enterohemorrhagic Escherichia coli ELISA, enzyme-linked immunosorbent assay ergic, endoplasmic reticulum-golgi intermediate compartment EUA, Emergency Use Authorization FDA, Food and Drug Administration FISH, fluorescent in situ hybridization G-CSF, (Granulocyte colony-stimulating facto) GQD, graphene quantum dot GSH, glutathione HBV, hepatitis B virus HCV, hepatitis C virus HIV, Human immunodeficiency virus HSV-1, herpes Simplex type-1 virus IC, inhibitory concentration ICU, Intensive Care Units IFN-γ, interferon gamma IGG, immunoglobulin g IGM, immunoglobulin m IP10, 10 Kda interferon gamma-induced protein LAMP, Loop-mediated isothermal amplification MagLev, magnetic levitation MB, methylene blue MCP1, monocyte chemoattractant protein-1 MERS-CoV, Middle East respiratory syndrome coronavirus MHV-A59, mouse coronavirus MWCNTs, multi-walled carbon nanotubes NagC, nano-silver colloids nanomedicine Nanoparticles PCR, polymerase chain reaction PDI, photodynamic inactivation PDT, photodynamic therapy PICALM, phosphatidylinositol binding clathrin assembly protein PS, photosensitizer PTAF, photocatalytic titanium apatite filter RCA, rolling circle amplification RdRp, RNA-dependent RNA polymerase RNA, Ribonucleic acid ROS, reactive oxygen species RPA, recombinase polymerase amplification RT-PCR, Reverse transcription polymerase chain reaction SARS-CoV, severe acute respiratory syndrome coronavirus SPION, superparamagnetic iron oxide nanoparticles Th2, T-helper-2 TNFα, Tumor necrosis factor alpha TPPS2a, tetraphenyl porphyrin disulphonate treatment protocols USFDA, United States Food And Drug Administration UV, ultraviolet WHO, World Health Organization
[DOI] 10.1016/j.pdpdt.2021.102287 PMC 바로가기 [Article Type] Review